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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Keeping CRISPR/Cas On-Target

by
Muhammad Jamal
1,
Faheem Ahmed Khan
2,
Lin Da
1,
Zeshan Habib
1,
Jinxia Dai
1,2,* and
Gang Cao
1,2,*
1
State Key Laboratory of Agriculture Microbiology, Huazhong Agricultural University, Wuhan 430070, China
2
College of Animal Science and Medicine, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2016, 20(1), 1-12; https://doi.org/10.21775/cimb.020.001
Submission received: 3 June 2015 / Revised: 9 July 2015 / Accepted: 9 August 2015 / Published: 11 October 2015
Versions Notes

Abstract

CRISPR/Cas, a microbial adaptive immune system, has recently been reshaped as a versatile genome editing approach, endowing genome engineering with high efficiency and robustness. The DNA endonuclease Cas, a component of CRISPR system, is directed to specific target within genomes by guide RNA (gRNA) and performs gene editing function. However, the system is still in its infancy and facing enormous challenges such as off-target mutation. Lots of attempts have been made to overcome such off-targeting and proven to be effective. In this review we focused on recent progress of increasing the CRISPR specificity realized by rational design of gRNA and modification of Cas9 endonuclease. Meanwhile the methods to screen off-target mutation and their effects are also discussed. Comprehensive consideration and rational design to reduce off-target mutation and selection of effective screening assay will greatly facilitate to achieve successful CRISPR/Cas system mediated gene editing.

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MDPI and ACS Style

Jamal, M.; Khan, F.A.; Da, L.; Habib, Z.; Dai, J.; Cao, G. Keeping CRISPR/Cas On-Target. Curr. Issues Mol. Biol. 2016, 20, 1-12. https://doi.org/10.21775/cimb.020.001

AMA Style

Jamal M, Khan FA, Da L, Habib Z, Dai J, Cao G. Keeping CRISPR/Cas On-Target. Current Issues in Molecular Biology. 2016; 20(1):1-12. https://doi.org/10.21775/cimb.020.001

Chicago/Turabian Style

Jamal, Muhammad, Faheem Ahmed Khan, Lin Da, Zeshan Habib, Jinxia Dai, and Gang Cao. 2016. "Keeping CRISPR/Cas On-Target" Current Issues in Molecular Biology 20, no. 1: 1-12. https://doi.org/10.21775/cimb.020.001

APA Style

Jamal, M., Khan, F. A., Da, L., Habib, Z., Dai, J., & Cao, G. (2016). Keeping CRISPR/Cas On-Target. Current Issues in Molecular Biology, 20(1), 1-12. https://doi.org/10.21775/cimb.020.001

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