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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

The Interactions of the Largest Subunit of RNA Polymerase II with Other Cellular Proteins: A Bioinformatic Approach

by
Abhijit Shukla
1,
Aparna Natarajan
1,
Sukesh Bhaumik
1,
Hany A. El-Shemy
2 and
David Lightfoot
1,*
1
Southern Illinois University School of Medicine, Department of Biochemistry and Molecular Biology, Carbondale, IL 62901, USA
2
Faculty of Agriculture Research Park (FARP) and Biochemistry Department, Faculty of Agriculture, University of Cairo, 12613 Giza, Egypt
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2009, 11(s1), 65-71; https://doi.org/10.21775/cimb.011.i65
Submission received: 5 August 2008 / Revised: 27 September 2008 / Accepted: 8 October 2008 / Published: 2 February 2009
Versions Notes

Abstract

The function of a protein is governed by its interaction with other proteins inside a cell. Therefore, it is important to identify the interacting partners of a particular protein to decipher its function. The protein interaction networks are generally determined by bioinformatic as well as experimental methodologies such as yeast two hybrid, mass spectrometry, immunoprecipitation, and fluorescence resonance energy transfer assays. Here, we have analyzed bioinformatically the interactions of Rpb1p (the largest subunit of RNA Polymerase II) with other proteins in yeast, using Cytoscape software and Biogrid/Biomart database. We find that Rpb1p interacts with a large number of proteins involved in mRNA synthesis, processing, export, and other cellular processes. These results validate the application of such bioinformatic approach to determine the interactome for other cellular proteins.
Keywords: bioinformatics; protein interactions; Rbp1; yeast; human bioinformatics; protein interactions; Rbp1; yeast; human

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MDPI and ACS Style

Shukla, A.; Natarajan, A.; Bhaumik, S.; El-Shemy, H.A.; Lightfoot, D. The Interactions of the Largest Subunit of RNA Polymerase II with Other Cellular Proteins: A Bioinformatic Approach. Curr. Issues Mol. Biol. 2009, 11, 65-71. https://doi.org/10.21775/cimb.011.i65

AMA Style

Shukla A, Natarajan A, Bhaumik S, El-Shemy HA, Lightfoot D. The Interactions of the Largest Subunit of RNA Polymerase II with Other Cellular Proteins: A Bioinformatic Approach. Current Issues in Molecular Biology. 2009; 11(s1):65-71. https://doi.org/10.21775/cimb.011.i65

Chicago/Turabian Style

Shukla, Abhijit, Aparna Natarajan, Sukesh Bhaumik, Hany A. El-Shemy, and David Lightfoot. 2009. "The Interactions of the Largest Subunit of RNA Polymerase II with Other Cellular Proteins: A Bioinformatic Approach" Current Issues in Molecular Biology 11, no. s1: 65-71. https://doi.org/10.21775/cimb.011.i65

APA Style

Shukla, A., Natarajan, A., Bhaumik, S., El-Shemy, H. A., & Lightfoot, D. (2009). The Interactions of the Largest Subunit of RNA Polymerase II with Other Cellular Proteins: A Bioinformatic Approach. Current Issues in Molecular Biology, 11(s1), 65-71. https://doi.org/10.21775/cimb.011.i65

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