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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Cloning of a Novel Antifungal Promoter from Phaseolus vulgaris and the Determination of its Activity in Stably Transformed Nicotiana tabacum Plants

by
Eman A. Mahmoud
1,
Solliman M. Mohei El-Din
1,
Mourad A. M. Aboul-Soud
2,
Ahmed M. Aboul-Enein
2,
Ghanem A. Sobhy
1 and
Hany A. El-Shemy
2,*
1
Centre of Excellence for Advanced Sciences, Plant Biotechnology Dept., National Research Centre, Dokki, Giza 12622, Egypt
2
Faculty of Agriculture Research Park (FARP) and Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2009, 11(s1), 55-63; https://doi.org/10.21775/cimb.011.i55
Submission received: 3 August 2008 / Revised: 22 October 2008 / Accepted: 31 October 2008 / Published: 2 February 2009
Versions Notes

Abstract

To investigate the transcriptional regulation of gene expression, chimeric fusions, between the beta-glucuronidase reporter gene (GUS) and the isolated promoter regions of the pvPDF gene (pvPDF-PRO: GUS), were constructed and introduced into Nicotiana tabacum. Analysis of transgenic pvPDF-PRO :GUS tobacco plants indicated that GUS activity was observed with all the promoter constructs with the strongest being in leaf followed by stem and roots. These results clearly demonstrate that pvPDF-PRO is a strong inducible and near-constitutive promoter and emphasize the great application potential for plant genetic engineering studies. Interestingly, a search for putative cis -acting elements in the pvPDF promoter architecture revealed the presence of some important transcription regulatory elements including: CAAT Box, TATA Box, CATA Box, and light regulatory elements (CCA1, GATA, GT-1). Taken together, these results further our understanding of the regulation of the pvPDF promoter activity.
Keywords: promoter isolation; cloning; Phaseolus vulgaris; defensins promoter isolation; cloning; Phaseolus vulgaris; defensins

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MDPI and ACS Style

Mahmoud, E.A.; El-Din, S.M.M.; Aboul-Soud, M.A.M.; Aboul-Enein, A.M.; Sobhy, G.A.; El-Shemy, H.A. Cloning of a Novel Antifungal Promoter from Phaseolus vulgaris and the Determination of its Activity in Stably Transformed Nicotiana tabacum Plants. Curr. Issues Mol. Biol. 2009, 11, 55-63. https://doi.org/10.21775/cimb.011.i55

AMA Style

Mahmoud EA, El-Din SMM, Aboul-Soud MAM, Aboul-Enein AM, Sobhy GA, El-Shemy HA. Cloning of a Novel Antifungal Promoter from Phaseolus vulgaris and the Determination of its Activity in Stably Transformed Nicotiana tabacum Plants. Current Issues in Molecular Biology. 2009; 11(s1):55-63. https://doi.org/10.21775/cimb.011.i55

Chicago/Turabian Style

Mahmoud, Eman A., Solliman M. Mohei El-Din, Mourad A. M. Aboul-Soud, Ahmed M. Aboul-Enein, Ghanem A. Sobhy, and Hany A. El-Shemy. 2009. "Cloning of a Novel Antifungal Promoter from Phaseolus vulgaris and the Determination of its Activity in Stably Transformed Nicotiana tabacum Plants" Current Issues in Molecular Biology 11, no. s1: 55-63. https://doi.org/10.21775/cimb.011.i55

APA Style

Mahmoud, E. A., El-Din, S. M. M., Aboul-Soud, M. A. M., Aboul-Enein, A. M., Sobhy, G. A., & El-Shemy, H. A. (2009). Cloning of a Novel Antifungal Promoter from Phaseolus vulgaris and the Determination of its Activity in Stably Transformed Nicotiana tabacum Plants. Current Issues in Molecular Biology, 11(s1), 55-63. https://doi.org/10.21775/cimb.011.i55

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