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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Antisense Phenotypes Reveal a Functional Expression of OsARF1, an Auxin Response Factor, in Transgenic Rice

by
Kotb A. Attia
1,2,
Amr F. Abdelkhalik
1,
Megahed H. Ammar
1,
Chun Wei
2,
Jinshui Yang
2,
David A. Lightfoot
3,
Wagih M. El-Sayed
4 and
Hany A. El-Shemy
5,*
1
Rice Biotechnology Lab., Rice Research and Training Center, Sakha, Kafr EL-Sheikh 33717, Egypt
2
Institute of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China
3
Plant Biotechnology and Genomics Core-Facility, Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901–4415, USA
4
Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
5
Faculty of Agriculture Research Park (FARP) and Biochemistry Department, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2009, 11(s1), 29-34; https://doi.org/10.21775/cimb.011.i29
Submission received: 13 August 2008 / Revised: 22 September 2008 / Accepted: 24 October 2008 / Published: 2 February 2009
Versions Notes

Abstract

OsARF1 is the first full-length member of auxin response factor (ARF) gene family to be cloned from monocot plant. Using quantitative RT-PCR this study found that, the transcript abundance of OsARF1 was significantly higher in embryonic tissues than in vegetative tissues. To investigate the effect of OsARF1 on the phenotype of rice, a cDNA fragment of OsARF1 was inserted in inverse orientation to the 35S promoter in vector pBin438 to produce an antisense (AS) construction. The AS-OsARF1 construct was transferred into rice (Oryza sativa L. japonica) calli via Agrobacterium tumefaciens -mediated transformation. Molecular analysis of transgenic plants showed that the functional expression of OsARF1 was inhibited at mRNA level efficiently. The AS-OsARF1 plants showed extremely low growth, poor vigor, short curled leaves and tillered but were sterile. Therefore, the OsARF1 was shown to be essential for growth in vegetative organs and seed development.
Keywords: auxin response factor 1(ARF1); antisense technology; gene transformation; functional genomics; Oryza sativa auxin response factor 1(ARF1); antisense technology; gene transformation; functional genomics; Oryza sativa

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MDPI and ACS Style

Attia, K.A.; Abdelkhalik, A.F.; Ammar, M.H.; Wei, C.; Yang, J.; Lightfoot, D.A.; El-Sayed, W.M.; El-Shemy, H.A. Antisense Phenotypes Reveal a Functional Expression of OsARF1, an Auxin Response Factor, in Transgenic Rice. Curr. Issues Mol. Biol. 2009, 11, 29-34. https://doi.org/10.21775/cimb.011.i29

AMA Style

Attia KA, Abdelkhalik AF, Ammar MH, Wei C, Yang J, Lightfoot DA, El-Sayed WM, El-Shemy HA. Antisense Phenotypes Reveal a Functional Expression of OsARF1, an Auxin Response Factor, in Transgenic Rice. Current Issues in Molecular Biology. 2009; 11(s1):29-34. https://doi.org/10.21775/cimb.011.i29

Chicago/Turabian Style

Attia, Kotb A., Amr F. Abdelkhalik, Megahed H. Ammar, Chun Wei, Jinshui Yang, David A. Lightfoot, Wagih M. El-Sayed, and Hany A. El-Shemy. 2009. "Antisense Phenotypes Reveal a Functional Expression of OsARF1, an Auxin Response Factor, in Transgenic Rice" Current Issues in Molecular Biology 11, no. s1: 29-34. https://doi.org/10.21775/cimb.011.i29

APA Style

Attia, K. A., Abdelkhalik, A. F., Ammar, M. H., Wei, C., Yang, J., Lightfoot, D. A., El-Sayed, W. M., & El-Shemy, H. A. (2009). Antisense Phenotypes Reveal a Functional Expression of OsARF1, an Auxin Response Factor, in Transgenic Rice. Current Issues in Molecular Biology, 11(s1), 29-34. https://doi.org/10.21775/cimb.011.i29

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