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Article

Updated Checklist of the Freshwater Shrimps (Decapoda: Caridea: Atyidae) of Mindoro Island, the Philippines, with a Description of a New Species of Caridina †

by
Valentin de Mazancourt
1,2,*,
Hendrik Freitag
1,3,
Kristina von Rintelen
1,
Marivene Manuel-Santos
4 and
Thomas von Rintelen
1
1
Center for Integrative Biodiversity Discovery, Museum für Naturkunde-Leibniz Institute for Evolution and Biodiversity Science, Invalidenstraße 43, 10115 Berlin, Germany
2
Unité Biologie des Organismes et Écosystèmes Aquatiques (UMR BOREA 8067), Muséum National d’Histoire Naturelle–CNRS–Université de Caen Normandie–Sorbonne Université–IRD–Université des Antilles, CP26, 57 Rue Cuvier, 75005 Paris, France
3
Biodiversity Laboratory, Department of Biology, Ateneo de Manila University, Katipunan Ave., Quezon City 1108, Philippines
4
Zoology Division, National Museum of Natural History–National Museum of the Philippines, P. Burgos Ave., Rizal Park, Manila 1000, Philippines
*
Author to whom correspondence should be addressed.
†
LSID urn:lsid:zoobank.org:pub:CFF11B0D-749A-41D7-81F1-ED88D84E7D3F
Arthropoda 2023, 1(4), 374-397; https://doi.org/10.3390/arthropoda1040015
Submission received: 9 June 2023 / Revised: 11 August 2023 / Accepted: 17 August 2023 / Published: 12 October 2023
(This article belongs to the Special Issue Alpha-Level Taxonomy of Decapod Crustaceans)
Browse Figures
Versions Notes

Abstract

:
Following recent expeditions to Mindoro Island, the Philippines, numerous samples of atyid shrimps were collected and then studied in an integrative taxonomy framework. A total of 16 species belonging to 4 genera are hereby reported, including 8 new records for the island and/or the Philippines: Atydina atyoides, Caridina bruneiana, C. celebensis, C. elongapoda, C. papuana, C. parvirostris, C. typus, C. zhujiangensis, and one new species, Caridina leptopoda sp. nov. Sequences of 16S rRNA have been produced for all of the species and taxonomical notes are provided. To account for the morphological variability across its range, C. bruneiana Choy, 1992 is herein re-described based on specimens from Mindoro. All of the species reported here from Mindoro have small eggs and are considered amphidromous, which suggests that they all potentially occur in other localities. This contrasts with other islands of the Philippines such as Bohol or Luzon where endemic species with large eggs can be found.

1. Introduction

Myers et al. (2000) [1] defined the Philippine archipelago as a biodiversity hotspot of global importance especially due to its taxa richness and high endemism rates. The Philippines’ biogeographic history is shaped by substantial landmass movements, collisions between landmasses of different origin in the Miocene, and temporary land bridges during Pleistocene sea-level regression maxima.
Mindoro, along with parts of Palawan and possibly portions of other adjacent areas, belongs to the oldest landmasses of the Philippines. Those areas are part of the so-called ‘Palawan Microcontinental Block’ or ‘Mindoro Block’ that separated from the margin of the Eurasian continent by the opening of the South China Sea (e.g., Hall 2002 [2], Zahirovic et al., 2014 [3]). A land connection with neighboring Luzon probably did not exist during the Pleistocene despite the proximity of the islands. This is somewhat supported by organismic and geological data (e.g., Shih et al., 2009 [4]; Esselstyn et al., 2009 [5]). Therefore, the island of Mindoro, including some small islands off-coast, is considered as its own intra-Philippine biogeographic region (Ong et al., 2002 [6]) with a specific, partly endemic arthropod freshwater fauna (Freitag et al., 2016 [7], Pelingen et al., 2020 [8], Ng and Takeda 1992 [9], Vidal et al., 2017 [10]).
The geography of the island is marked by a central mountain chain in a north–south direction (Figure 1), with its highest peak, Mt. Halcon (2585 m a.s.l.) causing a distinct climatic difference between the wetter eastern province of Oriental Mindoro compared to relatively dry Occidental Mindoro. The largest freshwater body is Lake Naujan (Figure 1) with about 8000 ha of open water and some associated wetlands. Butas River drains the lake and its catchment into the Tablas Straight. Although the island is comparably less populated (ca. 1.5 M inhabitants in 2020 (Philippine Statistics Authority 2022, [11]) and developed in terms of infrastructure, there are only very few remnants of primary forest while large parts of the island have been converted into cocoa plantations, paddy fields, slash-and-burn farmlands, and grasslands.
In the most recent studies of Philippine Atyidae (Cai and Shokita, 2006 [12]; Cai et al., 2009 [13], Han and Klotz, 2015 [14]), taxonomists indicate the presence of 36 species belonging to 8 genera: Antecaridina, Halocaridinides, Atyoida, Atyopsis, Australatya, Parisia, Edoneus with a single species each, and 29 species of Caridina. Some of the most species-rich islands are Bohol with 16 atyid species in 4 genera including 5 endemic Caridina (Cai et al., 2009 [13]) and Luzon with 13 species: 8 Caridina (including one endemic), 1 Parisia (endemic), and 4 Edoneus (all endemic) (Cai and Anker, 2004 [15]; Cai and Shokita, 2006 [12]; Cai and Husana, 2009 [16]). Other isolated endemic species of Caridina can be found on Cebu, Mindanao, and Palawan (one species each, Cai and Shokita, 2006 [12]). The earliest report of atyid shrimps from Mindoro is a species identified as Caridina nilotica (Roux, 1833) by Woltereck (1937) [17] collected from the northeast of the island (Suki River and Naujan Lake). The same year, Estampador (1937 [18] and 1959 [19]) reported two other species: Atya moluccensis De Haan, 1849 and Atya serrata Spence Bate, 1888 from the same region (Malayas River). Chace (1997) [20] provided a more complete record based on specimens collected during the Philippine expedition of the USS Albatross (1907–1910) by citing five species of the genus Caridina from the northwest tip of the island (Calawagan and Paluan Rivers): Caridina brevicarpalis endehensis De Man, 1892, C. gracilirostris De Man, 1892, C. longirostris H. Milne Edwards, 1837, C. serratirostris De Man, 1892, and C. villadolidi Blanco, 1939, in addition to Atyopsis spinipes (Newport, 1847) with some doubts regarding its exact sampling locality (“Varadero Mountain”, Mindoro (presumably a hill or small mountain in Puerto Galera near Varadero Bay)). Finally, the most recent and comprehensive account on atyids from Mindoro is that of Cai and Shokita (2006) [12] based on specimens collected during a Japanese crustacean expedition in 1985 in which they cite seven species: Atyopsis spinipes, Caridina laoagensis Blanco, 1939, C. villadolidi, C. brevicarpalis De Man, 1892, C. endehensis De Man, 1892, C. elongapoda Liang and Yan, 1977, and C. gracilipes De Man, 1892.
With the development of molecular taxonomy, species delimitations are becoming increasingly more precise, rendering past inventories uncertain when new pseudocryptic species keep being described. Based on new collections supported by previously published records, the present study aims to update the checklist of atyid shrimps from Mindoro Island with the description of a new species. The new and additional material treated herein is mainly from national key biodiversity areas (sensu Ong et al., 2002 [6]): “69 Hinunduang Mt.” (including parts of the Baroc River Catchment), “64 Naujan Lake National Park” and “62 Puerto Galera” of Mindoro which are recognized as priority areas for conservation and research of high or highest urgency. Many of the samples were retrieved by the Ateneo Biodiversity Laboratory (second author) through research and biodiversity teaching projects with students which have already led to several discoveries of new freshwater arthropods in the last decade (Freitag 2013 [21]; Garces et al., 2018 [22], 2020 [23]; Komarek and Freitag 2014 [24], 2020 [25]; Mey and Freitag 2013 [26]; Pelingen and Freitag 2020 [27]; Zettel and Pangantihon 2010 [28]).
Arthropoda 01 00015 g001
Figure 1. Map of the localities sampled on Mindoro listed in Table 1. Areas highlighted in red are Key Biodiversity Areas (BirdLife International, Adapted with the permission from Ref. [29]. Copyright 2022 Tom Lambert); Lake Naujan is in blue. Made with QGIS v. 2.1.
Figure 1. Map of the localities sampled on Mindoro listed in Table 1. Areas highlighted in red are Key Biodiversity Areas (BirdLife International, Adapted with the permission from Ref. [29]. Copyright 2022 Tom Lambert); Lake Naujan is in blue. Made with QGIS v. 2.1.
Arthropoda 01 00015 g001

2. Material and Methods

2.1. Sampling

Specimens were collected with the help of a fine-meshed hand net in various microhabitats from lentic and lotic areas of freshwater bodies on Mindoro. They were preserved in 96% ethylic alcohol.
The localities prospected are numbered from 1 to 40 (Figure 1) and listed in Table 1 with the species collected. Details on the collected specimens are available in the Supplementary Material.

2.2. Molecular Study

DNA was extracted from abdominal tissue using either a Qiagen Blood and Tissue Kit or a Qiagen BioSprint with the Plant Kit (but lysis with 10 mL Qiagen Proteinase K (20 mg/mL) added) according to the manufacturer’s instructions. Fragments of the mitochondrial 16S rRNA (16S, ~590 bp) were amplified by polymerase chain reaction (PCR) and sequenced using primers 16S-F-Car and 16S-R-Car1 (von Rintelen et al., 2007 [30]). Amplifications were conducted in 25 µL volumes containing 50–100 ng DNA, 1x PCR buffer, 200 mM of each dNTP, 0.5 mM of each primer, 2 mM MgCl2, and 1 U of Taq polymerase. After an initial denaturation step of 3 min at 94 °C, 35 cycles of 30 s at 94 °C, 60 s at 50 °C, and 60 s at 72 °C were performed, followed by a final extension step of 5 min at 72 °C. PCR products were sent to Macrogen Europe for purification and sequencing of both strands of the amplified gene fragments using the primers as given above.
Contigs of forward and reverse strands were assembled using Geneious Prime (v. 2019.2.1) and corrected by sight. The sequences were aligned using the Muscle algorithm (Edgar, 2004 [31]) implemented in MEGA 7 (Kumar et al., 2016 [32]). To determine the best substitution model for Bayesian inference analyses (see below), hierarchical likelihood ratio tests were carried out with jModelTest (Posada, 2008 [33]). Based on the Akaike inference criterion (AIC), the HKY + I + G model was retained.
All new sequences (77 from Mindoro species and 12 from other localities) have been deposited in GenBank (for accession numbers and museum voucher numbers, see Supplementary Table S1). The sequences were retrieved from GenBank to compile our dataset with other samples from Mindoro and samples close to the type locality of several species to support our identifications.
Phylogenetic trees were reconstructed by Bayesian inference (BI; Huelsenbeck et al., 2001 [34]) using MrBayes 3.2.7 (Ronquist and Huelsenbeck, 2003 [35]) implemented in the Cyber Infrastructure for Phylogenetic Research (CIPRES) portal v. 3.3 (Miller et al., 2010 [36]: https://www.phylo.org/ (accessed on 21 September 2023)). The MCMC algorithm was run with four independent chains for 10,000,000 generations, samplefreq = 1000, and burnin = 10%. Maximum likelihood (ML) analyses were run with RaxML-HPC2 (Stamatakis, 2014, [37]) and branch support was obtained through non-parametric bootstrapping (1000 replicates; Felsenstein, 1985 [38]). BI and ML analyses were run using the molecular evolution model specified above.
The dataset was analyzed using Assemble Species by Automatic Partitioning (ASAP) (Puillandre et al., 2021 [39]) to provide statistical support to our species hypotheses, following the phylogenetic species concept. This new method implements a hierarchical clustering algorithm that uses pairwise genetic distances and proposes species partitions ranked by a new scoring system (the lower the score, the better the partition) that uses no biological prior insight into intraspecific diversity.

2.3. Morphological Study

In order to identify species, update previously published descriptions, or describe new species, the specimens were studied under a Leica M205 C stereomicroscope and their appendages were dissected. Images were taken with a Leica DMC6200 and processed through LAS software (v. 4.13) with which measurements were taken following the protocol of Uebeler et al. (2022) [40]. Illustrations were made with Adobe Illustrator (v. 25.4.1) following the digital inking method from Coleman (2003 [41], 2006 [42]).
The following abbreviations are used in the present text: cl, carapace length (mm): measured from the post-orbital margin to the posterior margin of the carapace. P1: first pereiopod. P2: second pereiopod. P3: third pereiopod. P5: fifth pereiopod. Pl1: first pleopod. Pl2: second pleopod.

3. Results

3.1. Molecular Study

The phylogenetic analysis performed on the 16S marker (Figure 2) revealed 15 distinct clades corresponding to the morphospecies determined by the morphological study. These 15 morphospecies were supported by the ASAP analysis with a score of 6.5 (Supplementary Material).

3.2. Morphological Study

The morphological study allowed us to recognize the 15 clades as valid species and to produce the following identification key. This key serves for the determination of the species occurring on Mindoro but may also be useful when studying specimens from other Philippine islands.
1.1 First two pairs of pereiopods identical2 (Atya group)
1.2 First pair distinctly shorter than second3
2.1 Carapace with an acute projection on the pterygostomian margin, rostrum with
more than two teeth on the ventral margin		Atyopsis spinipes
2.2 Carapace with rounded pterygostomian margin, rostrum with fewer than two teeth
on the ventral margin		Atyoida pilipes
3.1 More than six post-orbital teeth situated on the dorsal margin of the rostrum. A long
stylocerite, reaching at least to the beginning of the second segment of the antennular peduncle		4 (C. serratirostris group)
3.2 Fewer than six dorsal rostrum teeth situated on the carapace behind the orbital
margin. A long or short stylocerite		5
4.1 Rostrum with 22–26 dorsal teeth and P2 carpus 8.2–10.9 times as long as
it is wide		Caridina serratirostris
4.2 Rostrum with 17–22 dorsal teeth and P2 carpus 11.9–12.0 times as long as
it is wide.		C. celebensis
5.1 Number of spiniform setae on the uropodal diaeresis 6–10 and a very long and
upcurved rostrum with 5–9 dorsal teeth, widely spaced		C. leptopoda sp. nov.
5.2 Number of spiniform setae on the uropodal diaeresis 8–22 and a long or short
rostrum with 8–28 dorsal teeth, closely set		6
6.1 Uropodal diaeresis with 14–22 spinules, short rostrum, armed or not, and high
unarmed pre-anal carina		7
6.2 Uropodal diaeresis with 8–17 spinules, rostrum variable in shape and length, and
low pre-anal carina with or without a spine		13
7.1 Telson with numerous and very long plumose intermediate setae8 (C. weberi group)
7.2 Telson with few long intermediate setae11 (C. typus group)
8.1 Rostrum without dorsal teethAtydina atyoides
8.2 Teeth present on the dorsal margin of the rostrum9
9.1 Rostrum straight, 9–17 teeth on the dorsal marginC. laoagensis
9.2 Rostrum bent downwards, 8–10 teeth on the dorsal margin10
10.1 Outer terminal projection of uropodal diaeresis shorter than the first movable spineC. parvirostris
10.2 Outer terminal projection of uropodal diaeresis longer than the first movable spineC. papuana
11.1 Rostrum reaching to or longer than the antennular peduncleC. villadolidi
11.2 Rostrum shorter than the antennular peduncle12
12.1 Rostrum reaching the end of the second segment of the antennular peduncle C. typus
12.2 Rostrum reaching the end of the first segment of the antennular peduncle C. zhujiangensis
13.1 No subapical teeth on the rostrum, tip of the rostrum rounded, and P1 carpus
deeply excavated		14 (C. brevicarpalis group)
13.2 Subapical teeth often present on the rostrum and tip of the rostrum pointed P1
carpus not deeply excavated		15
14.1 Rostrum overreaching the scaphoceriteC. endehensis
14.2 Rostrum reaching the end of the antennular peduncleC. brevicarpalis
15.1 Presence of one or few subapical teeth on the rostrum, fewer than two post-orbital teeth16 (C. nilotica group)
15.2 No subapical teeth on the rostrum, more than three post-orbital teethC. bruneiana
16.1 Distinct projection on the pre-anal carinaC. gracilipes
16.2 Absence of projection, sometimes the presence of a cluster of setaeC. elongapoda

3.3. Taxonomy: Updated Checklist of the Species of Atyid Shrimps from Mindoro Island

Family Atyidae De Haan, 1844
“Atya” group
Genus Atyoida Randall, 1840
Atyoida pilipes (Newport, 1847)
Restricted synonymy (see Chace, 1983 for a complete list):
  • Atya pilipes Newport, 1847 [43]: 160 (Type locality: Apia, Upolu, Samoa).
  • Atyoida pilipes—Chace, 1983 [44]: 13, figs. 3, 4 (part); 1997: 4.
  • Caridina acuminata Stimpson, 1860 [45]: 29 (Type locality: Ogasawara (Bonin) Islands, Japan).
  • Caridina brevirostris Stimpson, 1860 [45]: 29 (Type locality: Okinawa (Loo Choo) Island, Ryukyu Islands, Japan).
  • Atya brevirostris De Man, 1892 [46]: 360, 520, pl. 21: figs. 21, 21a–d (Type locality: Indonesia, Flores, several localities, and Timor, Koinino (=Kuanino) River near Kupang).
  • Pseudatya beauforti Roux, 1928 [47]: 209, figs. 1–9 (Type locality: Indonesia, Bacan Island).
  • Non Atya serrata—Blanco, 1935 [48]: 31.—Estampador, 1937 [18]: 485; 1959 [19]: 19.
Habitat: This species is found in areas with strong currents, usually in the higher course of rivers.
Distribution: Wide distribution in the Pacific Ocean: Japan (Ryukyu), Taiwan, the Philippines, Malaysia, Indonesia, Melanesia, Micronesia, and Polynesia (Samo and, Marquesas).
Remarks:
Reported from Mindoro under the name Atya serrata by Estampador (1937 [18], 1959 [19]). Not represented in our collections.
This species has been assessed as Least Concern for the IUCN Red List (De Grave et al., 2013 [49]).
 
Genus Atyopsis Chace, 1983
Atyopsis spinipes (Newport, 1847)
Restricted synonymy (see Chace, 1983 for a complete list):
  • Atya spinipes Newport, 1847 [43]: 159 (Type locality: Philippine Islands).
  • Atyopsis spinipes—Chace, 1983 [44]: 35, figs. 20–22.
  • Atya dentirostris Thallwitz, 1891 [50]: 101 (Type locality: North Celebes).
  • Atya brevirostris var. De Mani Nobili, 1900 [51]: 475, fig. 2. (Type locality: Indonesia, Mentawei Islands, Sipora Island, Sereinu (=Saurenu?) river).
  • Non Atya moluccensis—Estampador, 1937 [18]: 485; 1959 [19]: 18.
Habitat: This species inhabits areas with strong water currents, usually in the higher course of rivers.
Distribution: On Mindoro: Locality no. 38. Wide distribution in the Pacific Ocean: Japan (Ryukyu), Taiwan, the Philippines, Indonesia, Melanesia, Micronesia, and Samoa.
Remarks:
Reported from Mindoro under the name Atya moluccensis by Estampador (1937 [18], 1959 [19]).
This species has been assessed as Least Concern for the IUCN Red List (De Grave et al., 2013 [52]).
 
“Caridina” group
Genus Caridina H. Milne Edwards, 1837
“Caridina nilotica” species group
Caridina gracilipes De Man, 1892
  • Restricted synonymy:
  • Caridina Wyckii var. gracilipes De Man, 1892 [46]: 387, pl. 24, figs. 29e–k (Type locality: River near Maros, Sulawesi, Indonesia).
  • Caridina nilotica var. gracilipes—De Man, 1908 [53]: 270, pl. 20, fig. 7.
  • Caridina gracilipes—Cai and Shokita, 2006 [12]: 250.—de Mazancourt et al., 2018: 438 [54], fig. 6.—Cai, 2020 [55]: 1407, figs. 1–3.
  • Caridina nilotica var. bengalensis De Man, 1908 [53]: 265, pl. 20, figs. 6–6b (Type locality: Port Canning, Lower Bengal, India).
  • Caridina acuticaudata Dang, 1975 [56]: 70, fig. 4 (Type locality: Boi River, Hoa Binh Province, Vietnam).
  • Non-Caridina longirostris—Chace, 1997 [20]: 14 (part), fig. 7.
Habitat: This species occurs in the middle to lower course of rivers among submerged vegetation.
Distribution: On Mindoro: Localities no. 2, 3, 9, 13, and 39. China (Fujian, Guangdong, Guangxi, and Hainan), Taiwan, Vietnam, Indonesia, Singapore, Malaysia, the Philippines, Papua New Guinea, Solomon Islands, Australia, India, and Sri Lanka.
Remarks:
Previously reported from this island by Cai and Shokita (2006) [12] and by Chace (1997) [20] under the name Caridina longirostris. de Mazancourt et al. (2018) [54] and Cai (2020) [55] recently re-described this species in detail. In contrast to the findings of Cai and Shokita (2006) [12] and Cai (2020) [55], male specimens of this species have been found with a fully developed appendix interna on their endopod of the first pleopod. This character should thus be used with caution when identifying species of the C. nilotica group.
This species has been assessed as Least Concern for the IUCN Red List (Cai et al., 2013) [57].
 
Caridina elongapoda Liang and Yan, 1977
  • Caridina nilotica elongapoda Liang and Yan, 1977 [58]: 220, figs. 5–8.—Liang and Zheng, 1988: 15 (Type locality: Xinzai, Gulei village, Zhangpu County, Fujian, southern China).
  • Caridina elongapoda—Wowor et al., 2004 [59]: 341, fig. 6A, B.—Cai and Shokita, 2006 [12]: 249 (part?).—Cai et al., 2007 [60]: 285; 2009: 68.—Cai, 2014 [61]: 214; 2020 [55]: 1414, fig. 5.—de Mazancourt et al., 2018 [54]: fig. 2.
  • Caridina aff. brachydactyla—Yeo, Cai and Ng, 1999 [62]: 218, figs. 10–14.
  • Non Caridina longirostris—Chace, 1997 [20]: fig. 6 (part?).
Material examined:
PHILIPPINES—2 specimens; Puerto Galera, small creek; N 13°27′44″ E 120°59′11″; 02.07.2017; coll. Freitag; ZMB 30705 (DNA: 3078) and ZMB 30703 (DNA: 2160).
Comparative material:
MALAYSIA: 1 ♂, cl 4.2 mm; Pulau Tioman, Sungai Asah; 24.06.1997; coll. Ng et al.; ZRC 1998.0865.—1 ♀, cl 4.3 mm, same data as for preceding; (ZRC 1998.0865); 1 ♀ ovig., cl 4.3 mm, same data as for preceding (ZRC 1998.0865).
Habitat: This species lives in the lower course of rivers among submerged vegetation.
Distribution: On Mindoro: Localities no. 5 and 37. Southern China, Vietnam, Malaysia, Indonesia, and the Philippines.
Remarks:
Cai and Shokita (2006) [12] reported this species from Mindoro and provided a diagnosis. Re-examination of specimens cited by Yeo et al. (1999) [62] from Pulau Tioman (Malaysia) confirmed that they are conspecific with our specimens from Mindoro.
This species has been assessed as Least Concern for the IUCN Red List (De Grave, 2013 [63]).
 
Species group uncertain
Caridina bruneiana Choy, 1992
(Figure 3)
In order to account for the morphological variability across its range, C. bruneiana Choy, 1992 is re-described here based on specimens from Mindoro. This re-description combined with the DNA analysis enabled us to remove all remaining doubts regarding the validity of this species.
  • Caridina bruneiana Choy, 1992 [64]: 49, Figs. 1–4 (Type locality: Negara Brunei Darussalam, on the upper reaches of Temburong River at Batang Duri, 04°36′05″ N 115°06′45″ E, altitude approx. 33 m).
  • Caridina bruneiana—Wowor et al., 2004 [59]: 343 (key), Fig. 7A–C; Cai et al., 2007 [60]: 284.
Material examined:
PHILIPPINES—2 ♂ cl 2.8–3.1 mm, 2 ♀ ov. cl 4.3–4.4 mm; Roxas, Baroc River, Oriental Mindoro; 12.6169, 121.4031; 02.04.2013; coll. Freitag; ZMB 32805 (DNA 3161 and 3162).
Comparative material:
Caridina bruneiana Choy, 1992
BRUNEI—3 specimens; Temburong River tributary Sungai Seluju, small tributary near UBD field station; 4.5638, 115.1486; 90 m a.s.l.; 10.2018; coll. H. Freitag; ZMB 34587 (DNA 3421 and 3423).
Caridina sumatrensis De Man, 1892
INDONESIA—Syntype, 1♀, cl 4.9 mm; Sumatra, Batak land, near Deli; 12.1890; coll. C. Moesch; MNHN-IU-2015-1758.—1 ♀ ovig., cl 5.1 mm; Java; NMB 6.II.b.—Non type, 1♀, cl 6.0 mm; ca. 5 km. S from Deli Tua, Sungai Seruai, Deli, N. E. Sumatra; 16.11.1984; coll. M. Kottelat; RMNH.CRUS.D.54754.
Description:
Cephalothorax. Antennal spine below the suborbital angle. Pterygostomian margin blunt. Rostrum (Figure 3m–o): straight or slightly bent downward, 0.7–0.9 of cl, shorter or as long as the antennular peduncle, armed with 21–26 teeth on the dorsal margin, 3–4 of them situated on the carapace behind the orbital margin, ventral margin with 3–4 teeth. Rostral formula: (3–4) 21–26/3–4.
Eyes well developed, anterior end reaching to 0.68 length of the basal segment of the antennular peduncle. Antennular peduncle 0.71(♀)–0.85(♂) times as long as the carapace. Second segment shorter or the same length as the third. Stylocerite reaching the middle of the basal segment of the antennular peduncle.
Pereiopods. Epipods on the first four pereiopods. P1 (Figure 3a): chela 2.2 times as long as wide, movable finger 4.1–4.8 times as long as wide, 1.6–1.8 times length of palm; carpus 1.8–2.2 times as long as wide. P2 (Figure 3b) more slender and longer than P1 with chela 2.6 times as long as wide: movable finger 4.6–6.2 times as long as wide, 1.7–2.1 times the length of the palm; carpus 3.7–4.7 times as long as wide. P3 (Figure 3c): slender, dactylus (Figure 3e) short, 2.8–3.0 times as long as wide (terminal spiniform seta included) with 4 spiniform setae on the flexor margin in addition to the terminal spiniform seta; propodus 12.7–14.0 times as long as wide, 5.3–6.5 times as long as the dactylus. P5 (Figure 3d): dactylus (Figure 3f) short, 2.4–3.1 times as long as wide with 16–21 spiniform setae on the flexor margin; propodus 16.1–17.2 times as long as wide and 6.0–9.1 times as long as the dactylus.
Abdomen. Third abdominal somite with moderately convex dorsal profile. Sixth abdominal somite about 0.52–0.61 the carapace length and 1.45–1.75 times as long as the fifth somite, reaching 0.7–0.95 times the length of the telson.
Telson (Figure 3i) with 2–4 pairs of dorsal spinules and one pair of dorsolateral spinules; posterior margin with a median process, rounded with 2–4 intermediate plumose setae shorter than the lateral ones, which bear thin lateral setulae on the inner margin.
Pl1 (Figure 3j): Endopod of male triangular, 2.2–2.4 times as long as wide, reaching 0.27–0.34 times the exopod, with an appendix on the subdistal outer margin which reaches beyond the distal end of the endopod by half of its length.
Pl2 (Figure 3k): Appendix masculina on the second pleopod reaching 0.51 times the length of the endopod; appendix interna reaching 0.35 times the appendix masculina.
Preanal carina (Figure 3g) unarmed, higher in females than in males, with few long setae.
Uropodal diaeresis (Figure 3h) weakly sigmoid, with 12–14 spinules.
Eggs (Figure 3l) small, 0.34–0.43 × 0.22–0.24 mm (undeveloped).
Habitat: This species is found in the middle course of rivers.
Distribution: Known from Brunei, Singapore, and Mindoro Island (the Philippines).
Remarks:
A combination of morphological characters makes the placement of this species within a species group difficult. Indeed, it can be brought close to the C. nilotica group as redefined by de Mazancourt et al. (2020) [65] with its antennal spine inferior to the suborbital angle, its sixth abdominal somite longer than half the carapace length, and its triangular endopod of the first male pleopod and its uropodal diaeresis with fewer than 15 spinules. However, the absence of apical teeth on the dorsal margin of its rostrum, its high pre-anal carina, and the absence of a dorsal hump on its third abdominal segment are characters more typical of the C. weberi and C. typus groups as defined by the same authors (de Mazancourt et al., 2020) [65]. Its telson, however, is unique by the setation on its distal margin with few intermediate plumose setae shorter than the lateral ones. Finally, its position within the molecular phylogeny presented here is poorly resolved, advocating further for its distinctiveness from all the other species groups.
From the high number of post-orbital teeth on the dorsal margin of the rostrum (3–4), this new species resembles Caridina sumatrensis, reported by Cai and Shokita (2006) [12] from Palawan Island. However, it can be easily distinguished by its antennal spine which is placed below the orbital angle (vs. fused), its higher number of dorsal teeth on the rostrum 21–26 (vs. 15–22), the lower number of intermediate plumose setae on the distal margin of the telson 2–4 (vs. 4–5) and intermediate setae being shorter than lateral ones (vs. intermediate longer than lateral), and the lower number of spinules on the diaeresis 12–14 (vs. 18–19). The probable sexual dimorphism of the pre-anal carina is interesting to note; the examination of more specimens from other localities will allow us to confirm it.
This is the first record of this species from the Philippines. It was originally described from Brunei and later found in Singapore (Cai et al., 2007 [60]). Since this species produces small-sized eggs, it is likely amphidromous and thus expected to have a wide distribution.
This species has been assessed as Least Concern for the IUCN Red List (De Grave and Cai, 2013 [66]).
 
“Caridina brevicarpalis” species group
Caridina brevicarpalis De Man, 1892
  • Caridina brevicarpalis De Man, 1892 [46]: 365 (key), 397–399, pl. 24, fig. 30a–e (Type locality: River near Palopo, Luwu, Sulawesi, Indonesia).
  • Caridina brevicarpalis—Ortmann, 1894 [67]: 11; 1894 [68]: 402 (key), 404.—Roux, 1904 [69]: 553.—Bouvier, 1912 [70]: 919; 1913 [71]: 463; 1925 [72]: 178–180, figs. 372–374.—Roux, 1928 [47]: 200–201.—Cai and Shokita, 2006 [12]: 248.—Page et al., 2007 [73]: 649, 653, fig. 2 (part, Borneo specimen).—de Mazancourt et al., 2017 [74]: 226, fig. 4 (part, Indonesian specimen).
  • Caridina brevicarpalis brevicarpalis—Chace, 1997 [20]: 8.
Habitat: This species prefers the lower course of rivers, sometimes in brackish conditions.
Distribution: On Mindoro: Localities no. 8, 9 and 13. Known with certainty from Indonesia (Sulawesi, Borneo, Ambon, and Waigeo) and the Philippines (Palawan, Mindanao, and Mindoro).
Remarks:
Measurements of our specimens from Mindoro fit within the variation reported in the original description of the type specimens by De Man (1892) [46] and the DNA sequences cluster with sequences from specimens collected in Sulawesi, the type locality of C. brevicarpalis. Cai and Shokita (2006) [12] reported this species for the first time from the Philippines and Mindoro and treated it as distinct from Caridina endehensis (see next species).
This species has been assessed as Least Concern for the IUCN Red List (De Grave et al., 2013 [75]).
 
Caridina endehensis De Man, 1892
  • Caridina brevicarpalis var. endehensis De Man, 1892 [46]: 399, pl. 24, fig. 30e (Type locality: River Ba near Endeh, Flores, Indonesia).
  • Caridina brevicarpalis var. endehensis—Bouvier, 1925 [72]: 34.—Roux, 1928 [47]: 218.—Blanco, 1935 [48]: 34, pl. 2, fig. 25.—Chace, 1997 [20]: 8, fig. 3.
  • Caridina endehensis—Wowor et al., 2004 [59]: 341, fig. 5M.—Cai and Shokita, 2006 [12]: 248.—Karge and Klotz, 2007 [76]: 70.—Cai et al., 2009 [13]: 67.
Habitat: This species prefers the lower course of rivers, sometimes in brackish conditions.
Distribution: On Mindoro: Localities no. 7, 8, 13, 16 and 18. Indonesia (Flores, Sulawesi, and Sumba) and the Philippines.
Remarks:
Our specimens from Mindoro agree well with the original description of the type specimens by De Man (1892) [46]. This species seems more common than the previous one and has been abundantly documented from the Philippines for a long time (see Blanco, 1935 [48]; Estampador, 1937 [18]; 1959 [19]; Chace, 1997 [20]; Cai and Shokita, 2006 [12]; Cai et al., 2009 [13]).
This species has been assessed as Least Concern for the IUCN Red List (De Grave et al., 2013 [77]).
 
“Caridina gracilirostris” species group
Caridina leptopoda sp. nov.
(Figure 4)
  • Non Caridina gracilirostris—Estampador, 1937 [18]: 485 (part?); 1959 [19]: 19 (part?).—Chace, 1997 [20]: 10 (part).
Material examined:
Holotype: PHILIPPINES—1 ♀, cl 5.9 mm; Municipality Victoria, Bgy. Malayas, W coast Naujan Lake, littoral Vallisneria beds, Oriental Mindoro; 13.1497, 121.3133; 22.02.2010; coll. Freitag, Pangantihon; NMCR-12001 (DNA 3311).
Paratypes: PHILIPPINES—1 ♀, cl 5.5 mm; same data as for holotype (DNA 3312).—1 ♀ ov., cl 4.8 mm, 1 ♂, cl 4.2 mm, 1 specimen; Imugaan River, W-coast Fabrica, Fabrica/Sagay Municipality, Negros Occidental; 10.8824; 123.3552; coll. Richter, Stelbrink; NMCR-12002 (1 ♂), ZMB 29569 (DNA 925, 926 and 927).
Description:
Cephalothorax. Antennal spine distinctly below the suborbital angle. Pterygostomian margin rounded. Rostrum (Figure 4p): long, curved upward, 1.6–1.8 of cl, much longer than the scaphocerite, armed with 8–11 teeth on the dorsal margin, 0–1 of them situated on the carapace behind the orbital margin, unarmed part length 0.5–0.8 of the armed part, ventral margin with 25–33 teeth. Rostral formula: (0–1) 8–11/25–33.
Eyes well developed, anterior end reaching 0.51 the length of the basal segment of the antennular peduncle. Antennular peduncle 0.76(♀)–0.94(♂) times as long as the carapace. Second segment longer than the third. Stylocerite 0.85–0.95 of the basal segment of the antennular peduncle.
Pereiopods. Epipods on first four pereiopods. P1 (Figure 4a): chela 2.1–2.2 times as long as wide, movable finger 3.5–3.8 times as long as wide, 1.2–1.4 times the length of the palm; carpus 1.9–2.2 times as long as wide. P2 (Figure 4b) more slender and longer than P1 with chela 2.3–2.6 times as long as wide: movable finger 4.1–5.3 times as long as wide, 1.5–1.8 times the length of the palm; carpus 3.6–5.2 times as long as wide. P3 (Figure 4c): slender, dactylus (Figure 4e) long, 3.5–5.1 times as long as wide (terminal spiniform seta included) with 10–11 spiniform setae on the flexor margin including the terminal spiniform seta; propodus 13.8–16.9 times as long as wide, 4.0–4.3 times as long as the dactylus. P5 (Figure 4d): dactylus (Figure 4f) 3.9–6.3 times as long as wide with 40–42 spiniform setae on the flexor margin; propodus 15.3–19.5 times as long as wide, 3.8–4.3 times as long as the dactylus.
Abdomen. Third abdominal somite with moderately convex dorsal profile. Sixth abdominal somite about 0.71 times the carapace length and 2.4–2.6 times as long as the fifth somite, reaching 0.98 the length of the telson.
Telson (Figure 4i–l) with 3–4 pairs of dorsal spinules and one pair of dorsolateral spinules; posterior margin with a median process, triangular with 1–2 pairs of lateral setae and 0–1 pair of short intermediate simple setae shorter than the lateral ones.
Pl1 (Figure 4m): Endopod of male triangular, 1.96 times as long as wide, reaching 0.22 time the exopod, without an appendix.
Pl2 (Figure 4n): Appendix masculina on the second pleopod reaching 0.53 the length of the endopod; appendix interna reaching 0.38 time the appendix masculina.
Preanal carina (Figure 4g) armed with an acute spine.
Uropodal diaeresis (Figure 4h) straight and short, with 7–8 spinules.
Eggs (Figure 4o) small, 0.43–0.44 × 0.27–0.28 mm (developed).
Habitat: This species is found in the lower course of rivers, sometimes in brackish conditions.
Distribution: On Mindoro: Locality no. 17. Known only from the Philippines (Mindoro, Negros).
Etymology: From the Greek leptos, “slender”, and podos, “foot”, referring to its long third and fifth pereiopods.
Remarks:
Reported from Mindoro as Caridina gracilirostris by Chace (1997) [20]. This new species indeed resembles C. gracilirostris as re-described by de Mazancourt et al. (2020) [65] from its long rostrum with few post-orbital teeth, its short P1 carpus, and its spine on the pre-anal carina but it can be easily distinguished by its longer P3 dactylus 3.5–5.1 (vs. 3.3–3.8) times as long as wide with more spiniform setae on its P3 dactylus 10–11 (vs. 7–10) and P5 dactylus 40–42 (vs. 26–33) and its longer P3 propodus 15.8–16.9 (vs. 11.7–14.0) times as long as wide. It also looks like Caridina neglecta from its long rostrum and its spine on the pre-anal carina but can be easily distinguished by the absence of appendix interna on the endopod of its first male pleopod, its shorter P1 and P2 carpi 1.9–2.2 (vs. 2.3–3.1) and 3.6–5.2 (vs. 5.2–6.4) times as long as wide, respectively, and its longer P3 dactylus 4.8–5.1 (vs. 3.3–4.7) with more spiniform setae 10–11 (vs. 5–7). This new species is also similar to Caridina gracilima as re-described by Cai and Ng (2007) [78] from its long dactyli on pereiopods 3 and 5 but they can be separated by its higher number of teeth on the ventral margin of its rostrum 25–33 (vs. 19–24), the presence of a spine on its pre-anal carina (vs. absent), and its smaller eggs 0.43–0.44 × 0.27–0.28 mm (vs. 0.55–0.66 × 0.35–0.40 mm). None of our male specimens show an appendix interna on the endopod of the first pleopod, whereas Chace (1997) [20] found a rudimentary one on three of the eight males he examined. One can hypothesize variation of this character depending on the breeding season of the species since Chace’s specimens were collected in December whereas ours were collected in May.
 
“Caridina weberi” species group
Caridina laoagensis Blanco, 1939
  • Caridina laoagensis Blanco, 1939 [79]: 390, pl. 2 (Type locality: Laoag River, Laoag, Ilocos Norte Province, Luzon, the Philippines).
  • Caridina laoagensis—Chace, 1997 [20]: 12, fig. 5.—Cai and Anker, 2004 [15]: 237, fig. 3.—Cai and Shokita, 2006 [12]: 248; 2006 [80]: 2141.—Cai et al., 2009 [13]: 67.—Inui et al., 2019 [81]: 3.—Dwiyanto et al., 2021 [82]: 339, fig. 2G, H.
Habitat: This species is found in the middle to lower course of rivers.
Distribution: On Mindoro: Localities no. 4, 5, 8, 10, 13, 14, 19–27, 29–36, 38, and 40. The Philippines, Indonesia, Taiwan, and Japan (Ryukyu).
Remarks:
This species seems to be the most common in the localities sampled for the present study as in the rest of the Philippines according to Cai et al. (2009) [13].
This species has been assessed as Least Concern for the IUCN Red List (De Grave and Cai, 2013 [83]).
 
Caridina papuana Nobili, 1905
  • Caridina Weberi var. papuana Nobili, 1905 [84]: 481, Pl. 12, fig. 1a,b (Type locality: Small forest stream, Stephansort, Madang Province, Papua New Guinea).
  • Caridina Weberi var. papuana—Bouvier, 1925 [72]: 246.
  • Caridina weberi var. papuana—Roux, 1917 [85]: 591; 1928 [47]: 204
  • Caridina weberi papuana—Roux, 1934 [86]: 221.
  • Caridina cf. weberi papuana—de Mazancourt et al., 2017 [74]: fig. 4.
  • Caridina sp. Sol 1—Page et al., 2007 [73]: 649, fig. 2.
  • Caridina papuana—de Mazancourt et al., 2019 [87]: 166, figs. 2–5; 2020 [65]: 46, figs. 2N, 14, 26E.
Habitat: This species is found in the middle to lower course of rivers.
Distribution: On Mindoro: Localities no. 4, 5, 6, 8, 10, and 14. Papua New Guinea (Papua, New Hanover, and Manus), Indonesia (Papua, Sulawesi, and Ceram), the Solomon Islands (Choiseul), the Philippines (Mindoro), and Taiwan.
Remarks:
This species is reported herein for the first time from the Philippines. De Mazancourt et al. (2020) [65] recently re-described this species in detail and designated a lectotype among its syntypes.
This species has been assessed as Least Concern for the IUCN Red List (De Grave et al., 2013 [88]).
 
Caridina parvirostris De Man, 1892
  • Caridina parvirostris De Man, 1892 [46]: 375, pl. 22, fig. 24 (Type locality: River near Bombang (=Boba?), Flores Island, Indonesia).
  • Caridina parvirostris—Ortmann, 1894 [68]: 404.—Bouvier, 1905 [89]: 76 (key); 1913 [71]: 465 (key).—de Mazancourt et al., 2019 [87]: 822, 834; 2020 [65]: 54, 60.
  • Caridina Weberi var. parvirostris—Bouvier, 1925 [72]: 244, figs. 567–570.
Habitat: This species is found in the middle to lower course of rivers.
Distribution: On Mindoro: Localities no. 1, 8, and 10. Indonesia (Flores, Sulawesi, and Sumba) and the Philippines.
Remarks:
This species is also reported herein for the first time from the Philippines. It was previously known from Indonesia only (Flores, Sulawesi, and Sumba islands). Our specimens agree well with the original description and figures provided by De Man (1892) [46].
This species has been assessed as Data Deficient for the IUCN Red List (De Grave et al., 2013 [90]).
 
Genus Atydina Cai, 2010
Atydina atyoides (Nobili, 1900)
  • Caridina atyoides Nobili, 1900 [51]: 478.—Bouvier, 1905 [89]: 78 (key); 1925 [72]: 256, figs. 587–592.—Roux, 1928 [47]: 205.—Chace, 1997 [20]; 7.
  • Atydina atyoides—Cai, 2010 [91]: 76, figs. 1, 2.
Material examined:
PHILIPPINES—1 specimen; small mountain river NE of summit, Ulasan, Bgy. Harrison, Paluan, Mt. Calavite, Occidental Mindoro; 13.4542, 120.425; 10.09.1998; coll. Freitag; ZMB (DNA 967).
Comparative material:
INDONESIA—Holotype: 1♂, cl 6.5 mm; Si Oban, Sipura Island, Mentawei Archipelago; coll. E. Modigliani; MSNG.—1♂, cl 10.7 mm and 1♀, cl 9.4 mm; River Teobak, Western Ceram, Moluccas; 02.1910; coll. de Beaufort; RMNH.CRUS.D.202877.
Habitat: This species is found in the middle course of rivers.
Distribution: On Mindoro: Locality no. 1. Indonesia (Si Oban and Ceram) and the Philippines.
Remarks:
New record for the Philippines. Despite being immature, our specimens agree well with the measurements of the holotype and the specimens from Ceram re-described by Cai (2010) [91]. Both molecular and morphological data point towards placing this species within the Caridina weberi species group. The taxonomy of the genus Caridina is under revision, it is still not clear whether the genus Atydina should become a junior synonym of Caridina or be extended to cover the entire Caridina weberi species group erected to generic status. For the time being, we choose to keep the status quo, awaiting the formal revision.
This species has been assessed as Data Deficient for the IUCN Red List (De Grave et al., 2013 [92]).
 
“Caridina serratirostris” species group
Caridina celebensis De Man, 1892
  • Caridina serratirostris var. celebensis De Man, 1892 [46]: 385, pl. 23, figs. 28f–h (Type locality: River near Palopo, Luwu, Sulawesi, Indonesia).
  • Caridina serratirostris var. celebensis—Bouvier, 1925 [72]: 220.
  • Caridina serratirostris koterai Kamita, 1951 [93]: 75, pl. 5, figs. A–G.
  • Caridina serratirostris celebensis—Kamita, 1961 [94]: 74.—Fujino, 1972 [95]: 8, fig. 12.—Shokita, 1975 [96]: 119.
  • Caridina celebensis—Hayashi, 1989 [97]: 376, figs. e, g.—Shokita, 2003 [98]: 250, fig. 19K.—Cai and Shokita, 2006 [12]: 247; 2006 [80]: 2140.—Karge and Klotz, 2007 [76]: 90.—Page et al., 2007 [73]: fig. 2.—von Rintelen et al., 2008 [99]: 2244, fig. 4.—de Mazancourt et al., 2020 [65]: 71, figs, 2B, 24.
  • Caridina leptocarpa Liang and Zheng, 1988 [100]: 15, figs. 1–91 (type locality: Fuzhou, 26°N 119°E, Min River, Fujian Province, China).
  • Caridina leptocarpa—Liang and Zhou, 1993 [101]: 231.
  • Paracaridina leptocarpa—Liang, 2004 [102]: 318, fig. 155.
  • Non Caridina serratirostris—Chace, 1997 [20]: 19, fig. 11.
Habitat: This species is found in the lower course of rivers with marine influence.
Distribution: On Mindoro: Localities no. 3, 8, 9, and 15. Japan, China, Taiwan, the Philippines, Malaysia, Indonesia, and the Solomon Islands.
Remarks:
de Mazancourt et al. (2020) [65] recently re-described this species in detail. Interestingly, Caridina serratirostris, a very common species related to C. celebensis is not represented in our collections from Mindoro but was reported by Cai and Shokita (2006) [12] from Mindanao and Palawan. Records of C. serratirostris by Chace (1997) [20] from various islands in the Philippines seem to be C. celebensis instead.
This species has been assessed as Least Concern for the IUCN Red List (De Grave et al., 2013 [103]).
 
“Caridina typus” species group
Caridina typus H. Milne Edwards, 1837
Restricted synonymy:
  • Caridina typus H. Milne Edwards, 1837 (in H. Milne Edwards, 1834–1840) [104]: 363, pl. 25bis, figs. 4, 5 (Type locality: Unknown, likely Mauritius).
  • Caridina typus—Bernardes et al., 2017 [105]: 1 (part, ARC clade).—de Mazancourt et al., 2017 [74]: 226, fig. 4; 2019 [87]: 167, figs. 2–5; 2020 [65]: 34, figs. 2D, 10.
  • Caridina typus forme typica Bouvier, 1925 [72]: 250, figs. 272–295.
  • Caridina exilirostris Stimpson, 1860 [45]: 29 (Type locality (neotype): Okuma River, Okinawa Island, Ryukyu Islands, Japan).
  • Caridina siamensis Giebel, 1863 [106]: 329 (Type locality: Siam).
  • Caridina typus forme caledonica Bouvier, 1925 [72]: 253, figs. 296–297 (Type locality: New Caledonia).
Habitat: This species is found from the lower to the higher course of rivers.
Distribution: On Mindoro: Localities no. 5, 12, and 14. Widespread in the Indo-Pacific region from South Africa to Fiji and Australia to Japan.
Remarks:
de Mazancourt et al. (2020) [65] recently re-described this species based on specimens from most of its distribution range.
This species has been assessed as Least Concern for the IUCN Red List (De Grave, 2013 [107]).
 
Caridina zhujiangensis Chen, Chen and Guo, 2018
  • Caridina zhujiangensis Q.-H. Chen, W.-J. Chen and Guo, 2018 [108]: 319, figs. 4–6 (Type locality: Near the Resort Hotel, Dong’ao Island, Guangdong Province, China (E 113°42′03″, N 22°01′06″, al. 19 m, stn. 4)).
  • Caridina zhujiangensis—Xu et al., 2020 [109]: 21, figs. 6, 7.—Chen et al., 2020 [110]: 18, figs. 6, 7.—Feng et al., 2021 [111]: 34, fig. 2.
  • Non Caridina typus—Bernardes et al., 2017 [105]: 1 (part, TAL clade).
Habitat: This species is found from the lower to the higher course of rivers.
Distribution: On Mindoro: Locality no. 28. China (Guangdong), Vietnam, Thailand, Malaysia, the Philippines (Mindanao, Mindoro, Samar, Luzon, and Palawan), and Indonesia (Sulawesi, Taliabu, and Papua).
Remarks:
Bernardes et al. (2017) [105] reported this single specimen from Mindoro under the name Caridina typus, belonging to their clade TAL. Recently published mitochondrial DNA sequences from specimens of C. zhujiangensis from the type locality (Xu et al., 2020 [109]) allowed us to confirm the identity of this specimen.
This species’ status has not been assessed for the IUCN Red List yet.
 
Caridina villadolidi Blanco, 1939
  • Caridina villadolidi Blanco, 1939 [79]: 389, pl. 1, figs. 1–9 (Type locality: Laoag River, Laoag, Ilocos Norte Province, Luzon, the Philippines).
  • Caridina villadolidi—Hung et al., 1993 [112]: 485, figs. 1B, 3.—Chace, 1997 [20]: 21, fig. 12.—Shy and Yu, 1998 [113]: 62.—Cai and Ng, 2001 [114]: 668, fig. 4a–e.—Liang, 2004 [102]: 156, fig. 74.—Cai and Shokita, 2006 [12]: 248.—Cai et al., 2009 [13]: 66.—Bernardes et al., 2017 [105]: sup. fig. 2.—Vijayamma et al., 2021 [115]: 404 (key).
  • Caridina typus var. longirostris De Man, 1892 [46]: 370: pl. 22, fig. 22f–I (junior homonym of Caridina longirostris H. Milne Edwards, 1837) (Type locality: River near Reo, Flores, Indonesia; River near Palopo, Sulawesi, Indonesia; Benteng, Saleyer, Indonesia).
Habitat: This species is found in the lower course of rivers.
Distribution: On Mindoro: Localities no. 2, 4, 36, and 38. The Philippines, Indonesia, Taiwan, and Sri Lanka.
Remarks:
This species has been assessed as Least Concern for the IUCN Red List (De Grave et al., 2013 [116])

4. Discussion

All of the species reported so far from Mindoro Island have small eggs and therefore have a supposedly amphidromous lifecycle (Hancock, 1998 [117]; Han et al., 2011 [118]). Indeed, with few exceptions, most of the species reported in the present work have a rather large known distribution in other islands of the Philippines and even other countries. This contrasts with the atyid fauna of other islands with a number of endemic species of Caridina producing large eggs, such as Bohol (Cai et al., 2009 [13]), Samar (Cai and Anker, 2004 [15]), or the neighboring island of Luzon with its endemic genus Edoneus (Cai and Husana, 2009 [16]). It is important to note, however, that most of these endemic species are stygobiotic and that on Mindoro, only surface waters have been sampled for atyids. These differences in life history have implications for the conservation of these species. The four species of Edoneus endemic to Luzon are listed by the IUCN as Critically Endangered (E. atheatus) or at least Vulnerable (De Grave et al., 2013 [119]), whereas in contrast, all the species listed in the present study are either listed as Least Concern or Data Deficient.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/arthropoda1040015/s1.

Author Contributions

Conceptualization, V.d.M., H.F., K.v.R. and T.v.R.; writing—original draft preparation, V.d.M.; writing—review and editing, V.d.M., H.F., K.v.R., M.M.-S. and T.v.R.; data curation, T.v.R.; collecting permit acquisition, M.M.-S., H.F. and T.v.R. All authors have read and agreed to the published version of the manuscript.

Funding

The first author was supported by a postdoctoral fellowship of the Alexander von Humboldt Foundation. This fruitful collaboration was facilitated through the project Biodiversity Teaching in a Philippine–Cambodian–German Network (BIO-PHIL) funded by the German Academic Exchange Service (DAAD project BIO-PHIL 57393541). A research visit of the second author to ZMB (hosted by K.v.R.) for work on Philippine atyid shrimps was kindly supported by the European Commission research funds of SYNTHESYS (DE-TAF-2770). This study was partly financed by the German Research Council (DFG) Grant Ri 1950/1-1 to K.v.R.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All studied specimens have been deposited in museum collections and are available from the researchers upon reasonable request. The DNA sequences produced for the present study were deposited in GenBank.

Acknowledgments

We like to thank the team of the Ateneo Biodiversity Research Laboratory (Ateneo de Manila University), especially Clister Pangantihon for their assistance with the field sampling and laboratory work. Recent field sampling on Mindoro and the exchange of specimens was made possible by the permission (Gratuitous Permits 0133-17, 0149-18, Export Commodity Clearance 2020-0026) of the Bureau of Fisheries and Aquatic Resources and underlying permits from various local governments units of Mindoro, PENRO Oriental Mindoro, Calapan, and the National Commission for Indigenous People (NCIP). Many thanks to Thomas Lambert (BirdLife International, Cambridge, UK) for his help in providing the Key Biodiversity Area data. Finally, we are thankful to the three anonymous reviewers who provided valuable comments to improve the manuscript.

Conflicts of Interest

The authors declare no conflict of interest regarding the present work.

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Figure 2. 16S rRNA Bayesian phylogenetic tree of the species collected from Mindoro. The numbers above the branches show the Bayesian posterior probabilities and under the branches, the ML bootstrap values. The scale bar indicates the substitution rate.
Figure 2. 16S rRNA Bayesian phylogenetic tree of the species collected from Mindoro. The numbers above the branches show the Bayesian posterior probabilities and under the branches, the ML bootstrap values. The scale bar indicates the substitution rate.
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Figure 3. Caridina bruneiana: (a) first pereiopod; (b) second pereiopod; (c) third pereiopod; (d) fifth pereiopod; (e) dactylus of the third pereiopod; (f) dactylus of the fifth pereiopod; (g) pre-anal carina; (h) uropodal diaeresis; (i) telson; (j) first male pleopod; (k) second male pleopod; (l) undeveloped eggs; (m,n) variations of the rostrum; and (o) cephalothorax. Male cl 5.6 mm ZMB 32805-2: (a–i,o); male cl 3.0 mm ZMB 32805-4: (j,k,n); ovigerous female cl 4.4 mm ZMB 32805-1: l, m.
Figure 3. Caridina bruneiana: (a) first pereiopod; (b) second pereiopod; (c) third pereiopod; (d) fifth pereiopod; (e) dactylus of the third pereiopod; (f) dactylus of the fifth pereiopod; (g) pre-anal carina; (h) uropodal diaeresis; (i) telson; (j) first male pleopod; (k) second male pleopod; (l) undeveloped eggs; (m,n) variations of the rostrum; and (o) cephalothorax. Male cl 5.6 mm ZMB 32805-2: (a–i,o); male cl 3.0 mm ZMB 32805-4: (j,k,n); ovigerous female cl 4.4 mm ZMB 32805-1: l, m.
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Figure 4. Caridina leptopoda sp. nov.: (a) first pereiopod; (b) second pereiopod; (c) third pereiopod; (d) fifth pereiopod; (e) dactylus of third pereiopod; (f) dactylus of fifth pereiopod; (g) pre-anal carina; (h) uropodal diaeresis; (i) telson; (j–l) variations of the telson; (m) first male pleopod; (n) second male pleopod; (o) developed eggs; and (p) cephalothorax. Female paratype cl 5.5 mm: (a–i,k,p); male paratype cl 4.2 mm ZMB 29569-2: (j,m,n); ovigerous female paratype cl 4.8 mm ZMB 29569-1: (l,o).
Figure 4. Caridina leptopoda sp. nov.: (a) first pereiopod; (b) second pereiopod; (c) third pereiopod; (d) fifth pereiopod; (e) dactylus of third pereiopod; (f) dactylus of fifth pereiopod; (g) pre-anal carina; (h) uropodal diaeresis; (i) telson; (j–l) variations of the telson; (m) first male pleopod; (n) second male pleopod; (o) developed eggs; and (p) cephalothorax. Female paratype cl 5.5 mm: (a–i,k,p); male paratype cl 4.2 mm ZMB 29569-2: (j,m,n); ovigerous female paratype cl 4.8 mm ZMB 29569-1: (l,o).
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Table 1. List of the localities sampled on Mindoro with the species collected at each locality.
Table 1. List of the localities sampled on Mindoro with the species collected at each locality.
LocalityLocalityLatitudeLongitudeSpecies Found
1Paluan, Bgy. Harrison, small mountain river NE of summit Mt. Calavite13.4542120.425Atydina atyoides
Caridina parvirostris
2Mamburao, Bgy. Tayainaan, Tugilan River/Sitio, 1 km upstream of estuary 13.2308120.5817Caridina gracilipes
Caridina villadolidi
3Abra de Ilog, lowland creek in a rural area13.4603120.7644Caridina celebensis
Caridina gracilipes
4Puerto Galera, NR km 59, downstr. Aniuan Falls13.4862120.905Caridina laoagensis
Caridina papuana
Caridina villadolidi
5Puerto Galera, 8 km W of Muelle, Talipanan Riv.13.4894120.9056Caridina elongapoda
Caridina laoagensis
Caridina papuana
Caridina typus
6Puerto Galera, NR km 49, Tagbinai Munti River13.4833120.953Caridina papuana
7Puerto Galera, NR km 48.2, Tagbinai Malaki River13.4825120.9594Caridina endehensis
8Puerto Galera, NR km 37.2, downstream of Tamaraw Falls13.4508120.9908Caridina brevicarpalis
Caridina celebensis
Caridina cf. elongapoda
Caridina endehensis
Caridina laoagensis
Caridina papuana
Caridina parvirostris
9Puerto Galera, small creek13.4622120.9864Caridina brevicarpalis
Caridina celebensis
Caridina gracilipes
Caridina sp. ‘Palawan’
10Puerto Galera, Bisayaan River13.4333120.9731Caridina laoagensis
Caridina papuana
Caridina parvirostris
11Puerto Galera, Calsapa, lower Bisaan River13.4153120.9575Caridina serratirostris
12Baco, small creek13.3992121.0356Caridina typus
13Baco, Pinagsabangan River13.3322121.1275Caridina brevicarpalis
Caridina endehensis
Caridina gracilipes
Caridina laoagensis
Caridina serratirostris
14Baco, Tagbungan, Lantuyan mountain river13.3044121.0642Caridina laoagensis
Caridina papuana
Caridina typus
15Naujan, Bancuro13.2700121.3075Caridina celebensis
16Victoria, Malayas, Malayas River, W Naujan tributary13.1572121.3081Caridina endehensis
17Victoria, Malayas, W coast Naujan Lake13.1497121.3133Caridina leptopoda sp. nov.
18Sablayan, large mountain river12.8339120.9361Caridina endehensis
19Sablayan, SPPF, small limestone river12.7969120.9092Caridina laoagensis
20Bongabong, Formon, Pastuhan, Tangisan Falls12.7167121.3833Caridina laoagensis
21Bongabong, Lisap, lower Siange River12.6911121.3489Caridina laoagensis
22Roxas, San Vicente, Sitio Tauga Diit, Baroc River tributary Tauga Daka12.6347121.3258Caridina laoagensis
23Roxas, San Vicente, Sitio Taugad Diit, Baroc River tributary Taugad Daka12.6325121.3367Caridina laoagensis
24Roxas, San Vicente, Sitio Taugad Diit, Baroc River tributary Taugad Diit River12.6256121.3714Caridina laoagensis
25Roxas, San Vicente, Taugad River unnamed tributary12.6272121.3772Caridina laoagensis
26Roxas, San Vicente, Baroc River tributary Hiyong Creek12.6242121.3808Caridina laoagensis
27Roxas, San Vicente, Taugad River12.6183121.3970Caridina laoagensis
28Roxas, San Vicente, Baroc River12.6169121.4031Caridina zhujiangensis
29Roxas, Baroc River12.6169121.4031Caridina bruneiana
Caridina laoagensis
30Roxas, San Vicente, Sitio Quirao, Hinundungan River tributary Tagugoy Creek12.6083121.3772Caridina laoagensis
31Roxas, San Vicente, Sitio Quirao, Baroc River tributary Hinundungan River12.6064121.3914Caridina laoagensis
32Roxas, San Vicente, Sitio Quirao, Hinundungan River tributary Quirao Buhay Creek12.6028121.3833Caridina laoagensis
33Roxas, San Vicente, Sitio Quirao, Hinundungan River tributary Quirao na Balete Creek12.5939121.3928Caridina laoagensis
34Roxas, San Vicente, Sitio Tagaskan, Hinundungan River tributary Quianao Creek12.5889121.3611Caridina laoagensis
35Roxas, San Vicente, Upper Hinundungan River12.5861121.3600Caridina laoagensis
Caridina sp.
36Roxas, Baroc River12.5975121.4697Caridina laoagensis
Caridina villadolidi
37Roxas, Bagumbayan, Magugo River12.5928121.5175Caridina serratirostris
Caridina elongapoda
38San Jose, “Central”, Purok Tunnel, Busuanga River 12.4642121.0356Atyopsis spinipes
Caridina endehensis
Caridina laoagensis
Caridina villadolidi
39San Jose, Palangeran River12.3638121.1230Caridina gracilipes
Caridina serratirostris
40Bulalacao, Bagonsikat, Paluguan River12.3561121.2842Caridina laoagensis
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de Mazancourt, V.; Freitag, H.; von Rintelen, K.; Manuel-Santos, M.; von Rintelen, T. Updated Checklist of the Freshwater Shrimps (Decapoda: Caridea: Atyidae) of Mindoro Island, the Philippines, with a Description of a New Species of Caridina. Arthropoda 2023, 1, 374-397. https://doi.org/10.3390/arthropoda1040015

AMA Style

de Mazancourt V, Freitag H, von Rintelen K, Manuel-Santos M, von Rintelen T. Updated Checklist of the Freshwater Shrimps (Decapoda: Caridea: Atyidae) of Mindoro Island, the Philippines, with a Description of a New Species of Caridina. Arthropoda. 2023; 1(4):374-397. https://doi.org/10.3390/arthropoda1040015

Chicago/Turabian Style

de Mazancourt, Valentin, Hendrik Freitag, Kristina von Rintelen, Marivene Manuel-Santos, and Thomas von Rintelen. 2023. "Updated Checklist of the Freshwater Shrimps (Decapoda: Caridea: Atyidae) of Mindoro Island, the Philippines, with a Description of a New Species of Caridina" Arthropoda 1, no. 4: 374-397. https://doi.org/10.3390/arthropoda1040015

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