Next Article in Journal
Crop Wild Relatives (CWR) from Italy: Threatened Endemisms
Previous Article in Journal
Phenolic Compounds from Amaranthaceae Family as Potential Antitumor and Antibacterial Drugs
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:

The Marvellous Oregano Spices †

Mariana Oalđe Pavlović
Tanja Lunić
Marija Mandić
Ana Alimpić Aradski
Sonja Duletić-Laušević
1 and
Biljana Božić Nedeljković
Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, 11000 Belgrade, Serbia
Faculty of Biology, Institute of Physiology and Biochemistry “Ivan Đaja”, University of Belgrade, 11000 Belgrade, Serbia
Author to whom correspondence should be addressed.
Presented at the 2nd International Electronic Conference on Foods—Future Foods and Food Technologies for a Sustainable World, 15–30 October 2021; Available online:
Biol. Life Sci. Forum 2021, 6(1), 49;
Published: 14 October 2021


People are using plants with health-boosting properties, such as oregano species, in their daily diet. Nowadays, these plants, characterized as potential functional foods, are being extensively scientifically analyzed. Bearing that in mind, as well as the fact that neurodegenerative diseases such as Alzheimer’s and Parkinson’s are affecting millions of people worldwide, this study was defined to assess the antineurodegenerative, antineuroinflammatory, and neuroprotective potential of Origanum majorana (marjoram) and O. vulgare (oregano) from Serbia. The plant material was obtained from the Institute for Medicinal Plant Research “Dr. Josif Pančić”, Serbia, and the ethanolic extracts were prepared using classic maceration protocol. The acetylcholinesterase (AChE) and tyrosinase (TYR) inhibition protocols were used to examine antineurodegenerative activity. Microglial (BV2) cells stimulated with LPS were used to evaluate antineuroinflammatory activity in MTT, NBT, and Griess assays, while neuronal (SH-SY5Y) cells were used to determine the neuroprotective activity of the extracts in MTT and Griess assays. The results suggest that oregano extracts are slightly better at inhibiting AChE and TYR (68% vs. 61% at 0.1 mg/mL) than marjoram extracts (53% vs. 59% at 0.1 mg/mL), making them more active than the positive controls (galantamine 41% vs. kojic acid 25% at 0.1 mg/mL). Furthermore, these extracts normalized LPS-stimulated BV2 cells’ viability, while also reducing their production of inflammatory mediators, i.e., reactive oxygen species and nitric oxide, to the level of untreated cells. Additionally, supernatants of LPS-stimulated BV2 cells that were previously treated with these extracts normalized the viability of neurons compared to the control neurons (treated with supernatants of LPS-stimulated BV2 that were not previously treated with the extracts). Both extracts exhibited noticeable antineurodegenerative, antineuroinflammatory, and neuroprotective activities which represent powerful sources of phytochemicals with promising neuroprotective activity, which could be further examined for potential dietary supplement manufacturing.

Supplementary Materials

The following supporting information can be downloaded at:, Poster: The Marvellous Oregano Spices.

Author Contributions

Conceptualization, S.D.-L. and B.B.N.; methodology, M.O.P., A.A.A. and B.B.N.; validation, S.D.-L. and B.B.N.; formal analysis, M.O.P., T.L. and M.M.; investigation, M.O.P., T.L. and M.M.; resources, B.B.N.; data curation, M.O.P., T.L. and M.M.; writing—original draft preparation, M.O.P.; writing—review and editing, T.L., M.M., A.A.A., S.D.-L. and B.B.N.; visualization, M.O.P.; supervision, S.D.-L. and B.B.N.; project administration, B.B.N.; funding acquisition, B.B.N. All authors have read and agreed to the published version of the manuscript.


This research was funded by the Ministry of Education and Science of the Republic of Serbia, contract number 451-03-68/2020-14/200178.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Oalđe Pavlović, M.; Lunić, T.; Mandić, M.; Alimpić Aradski, A.; Duletić-Laušević, S.; Božić Nedeljković, B. The Marvellous Oregano Spices. Biol. Life Sci. Forum 2021, 6, 49.

AMA Style

Oalđe Pavlović M, Lunić T, Mandić M, Alimpić Aradski A, Duletić-Laušević S, Božić Nedeljković B. The Marvellous Oregano Spices. Biology and Life Sciences Forum. 2021; 6(1):49.

Chicago/Turabian Style

Oalđe Pavlović, Mariana, Tanja Lunić, Marija Mandić, Ana Alimpić Aradski, Sonja Duletić-Laušević, and Biljana Božić Nedeljković. 2021. "The Marvellous Oregano Spices" Biology and Life Sciences Forum 6, no. 1: 49.

Article Metrics

Back to TopTop