Abstract
The present study aimed to evaluate in vitro enzymatic activity of a novel biotechnological active complex based on natural origin compounds—thermophilic lipase and β-cyclodextrin—for hydrolysis of acylglycerides in plant oils and fats. β-cyclodextrin (β-CD) as an additive has attracted attention for its enhanced stability and efficiency of enzymes. In present study, the effects of β-CD on enzymatic hydrolysis of acylglycerides by thermophilic lipase were investigated by modern methods. The UV-spectroscopy, electron microscopy with TEM and kinetics of enzymatic hydrolysis were compared by the addition of β-CD. The results showed that lipase could produce the highest yield of oleic acid in presence of β-CD after 1 and 3 h. The UV spectroscopy demonstrated that the absorbance and fluorescence of lipase decreased with increasing concentration of β-CD due to surface interaction and change of enzyme configuration. Moreover, electron microscopy with TEM showed that lipase formed a special active conglomerate with β-CD for improving hydrolysis and stability. Dermatology evaluation indicated that this complex, when added to household products, did not affect sensitive skin of hands. Overall results indicate that β-CD could increase enzymatic activity of the lipase against acylglycerides and can be considered as promising composition in ecological household products for regular hand application.
Supplementary Materials
The presentation material of this work is available online at https://www.mdpi.com/article/10.3390/IECBM2022-13404/s1.
Author Contributions
Conceptualization, V.A.F. and G.G.E.; methodology, V.A.F.; software, V.A.F.; validation, V.A.F. and G.G.E.; formal analysis, V.A.F.; investigation, V.A.F.; resources, V.A.F.; data curation, V.A.F.; writing—original draft preparation, V.A.F. and G.G.E.; writing—review and editing, V.A.F.; visualization, V.A.F.; supervision, V.A.F.; project administration, V.A.F. and G.G.E.; funding acquisition, V.A.F. All authors have read and agreed to the published version of the manuscript.
Funding
This research was entirely provided by SkyLab AG (Switzerland).
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
The data presented in this study are available on request from the corresponding author. The data are not publicly available due to internal privacy policy.
Conflicts of Interest
The funder SkyLab AG has a role in the funding, design of study, in collection, analysis and interpretation of data, writing of the manuscript and decision to publish the results. V.A.F. and G.G.E. are employees of SkyLab AG. The authors declare no conflict of interest.
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