Changes in Morphology of Hepatocytes and Tissue-Biodistribution in Iron-Overload Gilthead Seabream (Sparus aurata) †
by
, , and
Jhon A. Serna-Duque
1
,
José Carlos Campos-Sánchez
1,‡,
Cristóbal Espinosa Ruiz
1,
Salvadora Martínez-López
2 and
Maria Ángeles Esteban
1,* 1
Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
2
Department of Agricultural Chemistry, Geology and Pedology, Faculty of Chemistry, University of Murcia, 30100 Murcia, Spain
*
Author to whom correspondence should be addressed.
†
Presented at the IX Iberian Congress of Ichthyology, Porto, Portugal, 20–23 June 2022.
‡
Presenting author (Poster presentation).
Biol. Life Sci. Forum 2022, 13(1), 82; https://doi.org/10.3390/blsf2022013082
Published: 13 June 2022
(This article belongs to the Proceedings of The IX Iberian Congress of Ichthyology)
Abstract
:Iron is an essential metallic micronutrient for life, but its bioavailability is especially scarce in marine areas. We have studied iron accumulation in gilthead seabream (Sparus aurata) to improve the knowledge of iron metabolism in fish. For this aim, acute iron overload was induced by intraperitoneally injection of iron-dextran. Nine different tissues were collected at 72 h after injection. Iron determination was performed in all samples by atomic absorption spectroscopy and liver was stained with hematoxylin–eosin for histologic study. Iron overload provoked big changes in the organization of the liver and, in particular, in the hepatocytes, which presented iron-deposits as hemosiderin and an increase of vacuolation by metabolic stress. Likewise, iron concentration in the studied samples indicated great changes in iron-distribution in tissues. In the control group, intestine, gill and blood are the predominant tissues in total proportion of iron in fish body, while iron overload produced a large increase in liver and spleen. In this way, the liver and spleen are storage organs of iron, and this is accumulated in the cytoplasm of hepatocytes. Hence, the study of iron accumulation has contributed to understand the iron-physiology and open doors to applications in aquaculture.
Author Contributions
Term, J.A.S.-D.; Conceptualization, M.Á.E.; methodology, J.A.S.-D., C.E.R. and S.M.-L.; software, J.A.S.-D. and S.M.-L.; validation, C.E.R. and M.Á.E.; formal analysis, C.E.R.; investigation, J.A.S.-D., C.E.R. and S.M.-L.; resources, M.Á.E.; data curation, C.E.R.; writing—original draft preparation, all authors; writing—review and editing, all authors; visualization, J.A.S.-D.; supervision, C.E.R. and M.Á.E.; project administration, M.Á.E.; funding acquisition, M.Á.E. All authors have read and agreed to the published version of the manuscript.
Funding
J.A.S.-D. has a grant (FPU19/02192). Project PID2020-113637RB-C21 of 346 research funded by MCIN/AEI/10.13039/501100011033.
Institutional Review Board Statement
All animal studies were carried out in accordance with the European Union regulations for animal experimentation and the Bioethical Committee of the University of Murcia.
Informed Consent Statement
Not applicable.
Conflicts of Interest
The authors declare no conflict of interest.
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MDPI and ACS Style
Serna-Duque, J.A.; Campos-Sánchez, J.C.; Espinosa Ruiz, C.; Martínez-López, S.; Esteban, M.Á. Changes in Morphology of Hepatocytes and Tissue-Biodistribution in Iron-Overload Gilthead Seabream (Sparus aurata). Biol. Life Sci. Forum 2022, 13, 82. https://doi.org/10.3390/blsf2022013082
AMA Style
Serna-Duque JA, Campos-Sánchez JC, Espinosa Ruiz C, Martínez-López S, Esteban MÁ. Changes in Morphology of Hepatocytes and Tissue-Biodistribution in Iron-Overload Gilthead Seabream (Sparus aurata). Biology and Life Sciences Forum. 2022; 13(1):82. https://doi.org/10.3390/blsf2022013082
Chicago/Turabian StyleSerna-Duque, Jhon A., José Carlos Campos-Sánchez, Cristóbal Espinosa Ruiz, Salvadora Martínez-López, and Maria Ángeles Esteban. 2022. "Changes in Morphology of Hepatocytes and Tissue-Biodistribution in Iron-Overload Gilthead Seabream (Sparus aurata)" Biology and Life Sciences Forum 13, no. 1: 82. https://doi.org/10.3390/blsf2022013082
