Wet Bone Characteristics Persist in Buried Bone after 10 Weeks: Implications for Forensic Anthropology
Abstract
:1. Introduction
- How long within a 10-week period does bone maintain its wet properties and its composition after death?
- How does fracture morphology change macroscopically and microscopically in the first ten weeks?
2. Materials and Methods
2.1. Materials
2.2. Fracture Reproduction
2.3. Analytical Methods
2.3.1. Macroscopic Analysis
- The term splintering was used in this study to describe small layers of bone detaching fully or partially in a different manner than peeling or flaking (Figure 1).
2.3.2. Biomechanical Analysis
2.3.3. Microscopic Analysis
- Collagen fibre pull-outs in areas of delamination at the convex side of the bone;
- Grooving on the fractured edge/surface on a microscopic level;
- Periosteal tearing, due to grooving or string-like appearances of the periosteal surface;
- Subsurface microcracks along the surface of the fracture.
2.3.4. Fourier Transform Infrared Spectroscopy (FTIR)
2.3.5. Thermal Analysis
2.3.6. Statistical Analysis
3. Results
3.1. Deposition Environment
3.2. Buried Sample
3.2.1. Macroscopic Analysis
3.2.2. Biomechanical Analysis
3.2.3. Microscopic Analysis
3.2.4. Biophysical Characterisation
4. Discussion
4.1. Macroscopic Analysis
4.2. Biomechanical Analysis
4.3. Microscopic Analysis
4.4. FTIR
4.5. Thermal Analysis
4.6. Buried vs. Unburied
4.7. Recommendations and Limitations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Crystallinity Index | Carbonate-to-Phosphate Ratio | Mineral-to-Matrix Ratio |
---|---|---|
(565 cm−1 + 595 cm−1)/565 | ν2 Carbon/ν1ν3 Phosphate | ν1ν3 Phosphate/Amide1 |
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Maier, A.K.; Manzella, A.; Bonicelli, A.; Arnold, E.L.; Márquez-Grant, N.; Zioupos, P. Wet Bone Characteristics Persist in Buried Bone after 10 Weeks: Implications for Forensic Anthropology. Forensic Sci. 2023, 3, 491-505. https://doi.org/10.3390/forensicsci3030034
Maier AK, Manzella A, Bonicelli A, Arnold EL, Márquez-Grant N, Zioupos P. Wet Bone Characteristics Persist in Buried Bone after 10 Weeks: Implications for Forensic Anthropology. Forensic Sciences. 2023; 3(3):491-505. https://doi.org/10.3390/forensicsci3030034
Chicago/Turabian StyleMaier, Anna Katharina, Alessia Manzella, Andrea Bonicelli, Emily L. Arnold, Nicholas Márquez-Grant, and Peter Zioupos. 2023. "Wet Bone Characteristics Persist in Buried Bone after 10 Weeks: Implications for Forensic Anthropology" Forensic Sciences 3, no. 3: 491-505. https://doi.org/10.3390/forensicsci3030034