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Emerging Technologies in Mass Spectrometry-Based DNA Adductomics

Masonic Cancer Center and Department of Medicinal Chemistry, University of Minnesota, 2231 6th St. SE, Minneapolis, MI 55455, USA
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High-Throughput 2019, 8(2), 13; https://doi.org/10.3390/ht8020013
Received: 29 March 2019 / Revised: 19 April 2019 / Accepted: 9 May 2019 / Published: 14 May 2019
(This article belongs to the Special Issue Adductomics: Elucidating the Environmental Causes of Disease)
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Abstract

The measurement of DNA adducts, the covalent modifications of DNA upon the exposure to the environmental and dietary genotoxicants and endogenously produced electrophiles, provides molecular evidence for DNA damage. With the recent improvements in the sensitivity and scanning speed of mass spectrometry (MS) instrumentation, particularly high-resolution MS, it is now feasible to screen for the totality of DNA damage in the human genome through DNA adductomics approaches. Several MS platforms have been used in DNA adductomic analysis, each of which has its strengths and limitations. The loss of 2′-deoxyribose from the modified nucleoside upon collision-induced dissociation is the main transition feature utilized in the screening of DNA adducts. Several advanced data-dependent and data-independent scanning techniques originated from proteomics and metabolomics have been tailored for DNA adductomics. The field of DNA adductomics is an emerging technology in human exposure assessment. As the analytical technology matures and bioinformatics tools become available for analysis of the MS data, DNA adductomics can advance our understanding about the role of chemical exposures in DNA damage and disease risk. View Full-Text
Keywords: Carcinogens; DNA adducts; adductomics; mass spectrometry Carcinogens; DNA adducts; adductomics; mass spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Guo, J.; Turesky, R.J. Emerging Technologies in Mass Spectrometry-Based DNA Adductomics. High-Throughput 2019, 8, 13.

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