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Open AccessProceedings

Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa

Dipartimento di Medicina Sperimentale, Università della Campania “L. Vanvitelli”, 80138 Naples, Italy
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Presented at the 6th International Electronic Conference on Sensors and Applications, 15–30 November 2019; Available online: https://ecsa-6.sciforum.net/.
Proceedings 2020, 42(1), 19; https://doi.org/10.3390/ecsa-6-06537 (registering DOI)
Published: 22 January 2020
Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis.
Keywords: caput and cauda spermatozoa; µFT-IR microspectroscopy; protein; nucleic acid; lipid and carbohydrate components caput and cauda spermatozoa; µFT-IR microspectroscopy; protein; nucleic acid; lipid and carbohydrate components
MDPI and ACS Style

Portaccio, M.; Errico, S.; Chioccarelli, T.; Cobellis, G.; Lepore, M. Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa. Proceedings 2020, 42, 19.

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