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Irisin Reduces the Metabolic Rate of Beige Adipocytes †

Maria Vliora
Elisabetta Grillo
Paraskevi Sakellariou
Stefania Mitola
2 and
Andreas D. Flouris
FAME Laboratory, Department of Exercise Science, University of Thessaly, 42100 Trikala, Greece
Department of Molecular and Translational Medicine, University of Brescia, 25121 Brescia, Italy
Author to whom correspondence should be addressed.
Presented at the 9th Greek Conference of Biochemistry and Physiology of Exercise, Thessaloniki, Greece, 18–20 October 2019.
Proceedings 2019, 25(1), 13;
Published: 30 August 2019
(This article belongs to the Proceedings of The 9th Conference of Biochemistry and Physiology of Exercise)


AIM: Irisin is released in the circulation in response to exercise and may increase energy expenditure by stimulating the browning of white adipose tissue through UCP1 upregulation. We aimed to investigate the effects of treatment with human recombinant irisin on the metabolic profile of 3T3-L1 differentiated beige adipocytes. MATERIAL & METHOD: 3T3-L1 cells were differentiated into beige adipocytes in the presence of IBMX, dexamethazone and insulin in DMEM. To assess the mitochondrial respiration activity, an extracellular mitochondrial assay was performed after 2 h and 4 h of treatment with 20 nM irisin. UCP1 protein expression levels were assessed through Western blot analysis at baseline, 2 h, and 4 h. RESULTS: We observed that the oxygen consumption rate (OCR) of the differentiated cells was significantly reduced after 2 h of treatment compared to control, untreated cells (40.8 ± 34.1 vs. 71.6 ± 51.7 pmol/min/μg, p < 0.05, d = 0.69). Interestingly, OCR was rescued after 4 h of treatment (57.4 ± 49.2 pmol/min/μg, p < 0.05, d = 0.38). Similar results were observed for the proton leak-mediated respiration (baseline, 26.5 ± 15.1; 2 h, 8.7 ± 3.3; 4 h, 17.2 ± 2.3 pmol/min/μg, p > 0.05). The highest expression of UCP1 was observed after the 2-h treatment with irisin (Δ2 h—baseline = 1.7-fold, Δ4 h—baseline = 1.2-fold, p < 0.05). CONCLUSIONS: Based on these results, we conclude that treatment of beige adipocytes with irisin generated a reverse effect on the thermogenic process. Beige adipocytes are already highly metabolic active cells that work close to their maximal capacity. Further stimulation of the thermogenic pathway can decrease the metabolic profile of 3T3-L1 cells, which can be partially rescued after 4 h of stimulation.

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MDPI and ACS Style

Vliora, M.; Grillo, E.; Sakellariou, P.; Mitola, S.; Flouris, A.D. Irisin Reduces the Metabolic Rate of Beige Adipocytes. Proceedings 2019, 25, 13.

AMA Style

Vliora M, Grillo E, Sakellariou P, Mitola S, Flouris AD. Irisin Reduces the Metabolic Rate of Beige Adipocytes. Proceedings. 2019; 25(1):13.

Chicago/Turabian Style

Vliora, Maria, Elisabetta Grillo, Paraskevi Sakellariou, Stefania Mitola, and Andreas D. Flouris. 2019. "Irisin Reduces the Metabolic Rate of Beige Adipocytes" Proceedings 25, no. 1: 13.

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