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Proceeding Paper

Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip

1
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
2
Department of Engineering Sciences, Izmir Katip Celebi University, Izmir 35620, Turkey
Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018.
Proceedings 2018, 2(25), 1556; https://doi.org/10.3390/proceedings2251556
Published: 20 December 2018
(This article belongs to the Proceedings of The 2nd International Cell Death Research Congress)
In this work, we focused on the development of a novel point of care (POC) paper-based analytical microfluidic chip. The system has been developed for paper-based extraction, non-enzymatic amplification and electroanalytical detection of human papillomavirus (HPV). The device comprises paper extraction support material as well as paper-based detection tool. Herein, a special DNA modification method has been utilized to allow non-enzymatic amplification by using microbeads and silver nanoparticles labeled primers. The device is capable of extracting more than 10–100 copies per mL of DNA approximately in 15 min along with a single step extraction process from patient samples. In addition, it is able to detect low concentrations taking just less than 10 min with high selectivity to HPV kinds of 16&18. It only takes the low-cost point of care device less than 40 min with a low limit of detection (LOD) to complete the whole process.
Keywords: microfluidics; POC; paper analytical chip; HPV; cervical cancer; molecular diagnosis microfluidics; POC; paper analytical chip; HPV; cervical cancer; molecular diagnosis
MDPI and ACS Style

Karakaya, M. Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip. Proceedings 2018, 2, 1556. https://doi.org/10.3390/proceedings2251556

AMA Style

Karakaya M. Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip. Proceedings. 2018; 2(25):1556. https://doi.org/10.3390/proceedings2251556

Chicago/Turabian Style

Karakaya, Melike. 2018. "Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip" Proceedings 2, no. 25: 1556. https://doi.org/10.3390/proceedings2251556

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