The development of parasite resistance to first-line antimalarial medicines, especially the ACTs, has made the research and development of novel antimalarial medications vital. Globimetula oreophila, a plant used in traditional medicine to treat malaria, is an example of a natural product that may provide new antimalarial drugs with fewer side effects, less drug resistance, and greater efficacy than synthetic drugs. This study aims to evaluate the antiplasmodial properties of G. oreophila’s fractions. After collection and authentication, the leaves were air-dried and reduced in size using a pestle and mortar. The pulverized plant was macerated in 70% ethanol and fractionated with solvents in the increasing polarity of n-hexane, chloroform, ethyl acetate, and n-butanol to produce the various fractions. The antiplasmodial activity of the n-hexane, chloroform, ethyl acetate, and n-butanol fractions of Globimetula oreophila leaf extract was assessed using an in vivo method in Plasmodium berghei-infected mice. In the mice, the fractions’ median fatal dose (LD50) was calculated to be more than 5000 mg/kg. At doses of 125, 250, and 500 mg/kg, the fractions significantly (p < 0.001) reduced the parasitemia level. The fractions of G. oreophila showed significant in vivo antiplasmodial activity, which upholds the earlier in vivo findings for the crude extract as well as its folkloric use.
Supplementary Materials
The following are available online at https://www.mdpi.com/article/10.3390/proceedings2024105036/s1.
Author Contributions
Conceptualization, D.G., Y.M.S., M.G.M. and H.H.S.; methodology, D.G., Y.M.S., M.G.M. and H.H.S.; software, D.G., B.H.A. and B.B.; validation, D.G., A.S., M.G.M., Y.M.S. and H.H.S.; formal analysis, D.G., B.H.A., A.M.M., M.I.A., M.G.M. and Y.M.S.; investigation, D.G., Y.M.S., M.G.M., M.I.A. and H.H.S.; resources, D.G., A.S., B.B., M.G.M. and B.H.A.; writing—original draft preparation, D.G., Y.M.S., M.G.M. and H.H.S.; writing—review and editing, D.G., Y.M.S., M.G.M., H.H.S. and A.M.M.; supervision, Y.M.S., M.G.M. and H.H.S.; project administration, Y.M.S., M.G.M., H.H.S. and D.G. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review Board Statement
This study was conducted by the Declaration of Helsinki and approved by Ahmadu Bello University Zaria, Committee on Animal Use and Care (ABUCAUC) with an approval number: ABUCAUC/2023/061.
Informed Consent Statement
Not applicable.
Data Availability Statement
The data presented in this study are available on request from the corresponding author. The data are not publicly available due to ethical concerns about plagiarism.
Conflicts of Interest
The authors declare no conflicts of interest.
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