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Article

Assessing the Need for Multiplex and Multifunctional Tick-Borne Disease Test in Routine Clinical Laboratory Samples from Lyme Disease and Febrile Patients with a History of a Tick Bite

1
Tezted Ltd., Mattilaniemi 6-8, 40100 Jyväskylä, Finland
2
United Medix Laboratories, Kivihaantie 7, 00310 Helsinki, Finland
*
Authors to whom correspondence should be addressed.
Academic Editor: Natasha Rudenko
Trop. Med. Infect. Dis. 2021, 6(1), 38; https://doi.org/10.3390/tropicalmed6010038
Received: 23 February 2021 / Revised: 11 March 2021 / Accepted: 12 March 2021 / Published: 17 March 2021
Human polymicrobial infections in tick-borne disease (TBD) patients is an emerging public health theme. However, the requirement for holistic TBD tests in routine clinical laboratories is ambiguous. TICKPLEX® PLUS is a holistic TBD test utilized herein to assess the need for multiplex and multifunctional diagnostic tools in a routine clinical laboratory. The study involved 150 specimens categorized into Lyme disease (LD)-positive (n = 48), LD-negative (n = 30), and febrile patients from whom borrelia serology was requested (n = 72, later “febrile patients”) based on reference test results from United Medix, Finland. Reference tests from DiaSorin, Immunetics, and Mikrogen Diagnostik followed the two-tier LD testing system. A comparison between the reference tests and TICKPLEX® PLUS produced 86%, 88%, and 87% positive, negative, and overall agreement, respectively. Additionally, up to 15% of LD and 11% of febrile patients responded to TBD related coinfections and opportunistic microbes. The results demonstrated that one (TICKPLEX® PLUS) test can aid in a LD diagnosis instead of four tests. Moreover, TBD is not limited to just LD, as the specimens produced immune responses to several TBD microbes. Lastly, the study indicated that the screening of febrile patients for TBDs could be a missed opportunity at reducing unreported patient cases. View Full-Text
Keywords: Lyme disease; tick-borne disease; zoonoses; spirochetes; polymicrobial; summer flu; misdiagnosis; persister; Borrelia; Lyme diagnostic Lyme disease; tick-borne disease; zoonoses; spirochetes; polymicrobial; summer flu; misdiagnosis; persister; Borrelia; Lyme diagnostic
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MDPI and ACS Style

Garg, K.; Jokiranta, T.S.; Filén, S.; Gilbert, L. Assessing the Need for Multiplex and Multifunctional Tick-Borne Disease Test in Routine Clinical Laboratory Samples from Lyme Disease and Febrile Patients with a History of a Tick Bite. Trop. Med. Infect. Dis. 2021, 6, 38. https://doi.org/10.3390/tropicalmed6010038

AMA Style

Garg K, Jokiranta TS, Filén S, Gilbert L. Assessing the Need for Multiplex and Multifunctional Tick-Borne Disease Test in Routine Clinical Laboratory Samples from Lyme Disease and Febrile Patients with a History of a Tick Bite. Tropical Medicine and Infectious Disease. 2021; 6(1):38. https://doi.org/10.3390/tropicalmed6010038

Chicago/Turabian Style

Garg, Kunal, T. S. Jokiranta, Sanna Filén, and Leona Gilbert. 2021. "Assessing the Need for Multiplex and Multifunctional Tick-Borne Disease Test in Routine Clinical Laboratory Samples from Lyme Disease and Febrile Patients with a History of a Tick Bite" Tropical Medicine and Infectious Disease 6, no. 1: 38. https://doi.org/10.3390/tropicalmed6010038

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