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Concordance between Plasma and Filter Paper Sampling Techniques for the Lymphatic Filariasis Bm14 Antibody ELISA
by
Jesse Masson 1,*, Jan Douglass 1
, Maureen Roineau 1, Khin Saw Aye 2, Kyi May Htwe 2, Jeffrey Warner 1 and Patricia M. Graves 1
, Maureen Roineau 1, Khin Saw Aye 2, Kyi May Htwe 2, Jeffrey Warner 1 and Patricia M. Graves 1
1
College of Public Health, Medical and Veterinary Sciences, James Cook University, Cairns, QLD 4870, Australia
2
Department of Medical Research, Myanmar Ministry of Health and Sports, Nay Pyi Taw, Myanmar
*
Author to whom correspondence should be addressed.
Academic Editors: Peter Leggat and John Frean
Trop. Med. Infect. Dis. 2017, 2(2), 6; https://doi.org/10.3390/tropicalmed2020006
Received: 20 February 2017 / Revised: 27 March 2017 / Accepted: 4 April 2017 / Published: 7 April 2017
(This article belongs to the Special Issue Neglected and Emerging Tropical Diseases in South and Southeast Asia and Northern Australia)
Diagnostic testing for the antibody Bm14 is used to assess the prevalence of bancroftian and brugian filariasis in endemic populations. Using dried blood spots (DBS) collected on filter paper is ideal in resource-poor settings, but concerns have been raised about the performance of DBS samples compared to plasma or serum. In addition, two versions of the test have been used: the Bm14 CELISA (Cellabs Pty Ltd., Manly, Australia) or an in-house CDC version. Due to recent improvements in the CELISA, it is timely to validate the latest versions of the Bm14 ELISA for both plasma and DBS, especially in settings of residual infection with low antibody levels. We tested plasma and DBS samples taken simultaneously from 92 people in Myanmar, of whom 37 (40.2%) were positive in a rapid antigen test. Comparison of results from plasma and DBS samples demonstrated no significant difference in positive proportions using both the CELISA (46.7% and 44.6%) and CDC ELISA (50.0% and 47.8%). Quantitative antibody unit results from each sample type were also highly correlated, with coefficients >0.87. The results of this study demonstrate that DBS samples are a valid collection strategy and give equivalent results to plasma for Bm14 antibody ELISA testing by either test type.
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Keywords:
lymphatic filariasis; Bm14; filter paper; DBS; CELISA; CDC
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MDPI and ACS Style
Masson, J.; Douglass, J.; Roineau, M.; Aye, K.S.; Htwe, K.M.; Warner, J.; Graves, P.M. Concordance between Plasma and Filter Paper Sampling Techniques for the Lymphatic Filariasis Bm14 Antibody ELISA. Trop. Med. Infect. Dis. 2017, 2, 6.
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