Next Article in Journal
Metastasis Model of Cancer Stem Cell-Derived Tumors
Previous Article in Journal
Comparison of Opioid-Free Anesthesia Versus Opioid-Containing Anesthesia for Elective Laparoscopic Surgery (COFA: LAP): A Protocol Measuring Recovery Outcomes
Open AccessArticle

SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab

1
Namur Research Institute for Life Sciences (NARILIS), Integrated Veterinary Research Unit (URVI), University of Namur, 61 rue de Bruxelles, 5000 Namur, Belgium
2
KU Leuven, Laboratory of Clinical Bacteriology and Mycology, campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium
3
Department of Laboratory Medicine, National Reference Center for Respiratory Pathogens, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium
4
CHU UCL NAMUR, Laboratory of microbiology and molecular biology, Site Godinne, 5530 Yvoir, Belgium
*
Authors to whom correspondence should be addressed.
Equally contributing authors: Lize Cuypers: [email protected]
Methods Protoc. 2020, 3(3), 59; https://doi.org/10.3390/mps3030059
Received: 2 July 2020 / Revised: 12 August 2020 / Accepted: 13 August 2020 / Published: 18 August 2020
(This article belongs to the Section Biomedical Sciences and Physiology)
The emergence of the SARS-CoV-2 virus and the exponential growth of COVID-19 cases have created a major crisis for public health systems. The critical identification of contagious asymptomatic carriers requires the isolation of viral nucleic acids, reverse transcription, and amplification by PCR. However, the shortage of specific proprietary reagents or the lack of automated platforms have seriously hampered diagnostic throughput in many countries. Here, we provide a procedure for SARS-CoV-2 detection for diagnostic purposes from clinical samples in the setting of a basic research molecular biology lab. The procedure details the necessary steps for daily analysis of up to 500 clinical samples with a team composed of 12 experienced researchers. The protocol has been designed to rely on widely available reagents and devices, to cope with heterogeneous clinical specimens, to guarantee nucleic acid extraction from very scarce biological material, and to minimize the rate of false-negative results. View Full-Text
Keywords: SARS-CoV-2; diagnosis; COVID-19; RTqPCR SARS-CoV-2; diagnosis; COVID-19; RTqPCR
Show Figures

Figure 1

MDPI and ACS Style

Coupeau, D.; Burton, N.; Lejeune, N.; Loret, S.; Petit, A.; Pejakovic, S.; Poulain, F.; Bonil, L.; Trozzi, G.; Wiggers, L.; Willemart, K.; André, E.; Laenen, L.; Cuypers, L.; Van Ranst, M.; Bogaerts, P.; Muylkens, B.; Gillet, N.A. SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab. Methods Protoc. 2020, 3, 59. https://doi.org/10.3390/mps3030059

AMA Style

Coupeau D, Burton N, Lejeune N, Loret S, Petit A, Pejakovic S, Poulain F, Bonil L, Trozzi G, Wiggers L, Willemart K, André E, Laenen L, Cuypers L, Van Ranst M, Bogaerts P, Muylkens B, Gillet NA. SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab. Methods and Protocols. 2020; 3(3):59. https://doi.org/10.3390/mps3030059

Chicago/Turabian Style

Coupeau, Damien; Burton, Nicolas; Lejeune, Noémie; Loret, Suzanne; Petit, Astrid; Pejakovic, Srdan; Poulain, Florian; Bonil, Laura; Trozzi, Gabrielle; Wiggers, Laetitia; Willemart, Kévin; André, Emmanuel; Laenen, Lies; Cuypers, Lize; Van Ranst, Marc; Bogaerts, Pierre; Muylkens, Benoît; Gillet, Nicolas A. 2020. "SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research Lab" Methods Protoc. 3, no. 3: 59. https://doi.org/10.3390/mps3030059

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop