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Open AccessProtocol

Tracking Single Cells Motility on Different Substrates

1
Department of Biology, The Catholic University of America, Washington, DC 20064, USA
2
Department of Biomedical Engineering, The Catholic University of America, Washington, DC 20064, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Methods Protoc. 2020, 3(3), 56; https://doi.org/10.3390/mps3030056
Received: 2 July 2020 / Revised: 30 July 2020 / Accepted: 31 July 2020 / Published: 4 August 2020
(This article belongs to the Section Molecular and Cellular Biology)
Motility is a key property of a cell, required for several physiological processes, including embryonic development, axon guidance, tissue regeneration, gastrulation, immune response, and cancer metastasis. Therefore, the ability to examine cell motility, especially at a single cell level, is important for understanding various biological processes. Several different assays are currently available to examine cell motility. However, studying cell motility at a single cell level can be costly and/or challenging. Here, we describe a method of tracking random cell motility on different substrates such as glass, tissue-culture polystyrene, and type I collagen hydrogels, which can be modified to generate different collagen network microstructures. In this study we tracked MDA-MB-231 breast cancer cells using The CytoSMARTTM System (Lonza Group, Basel, Switzerland) for live cell imaging and assessed the average cell migration speed using ImageJ and wrMTrck plugin. Our cost-effective and easy-to-use method allows studying cell motility at a single cell level on different substrates with varying degrees of stiffness and varied compositions. This procedure can be successfully performed in a highly accessible manner with a simple setup. View Full-Text
Keywords: substrate stiffness; collagen extracellular matrix; single cell motility; live cell tracking; wrMTrck plugin; ImageJ substrate stiffness; collagen extracellular matrix; single cell motility; live cell tracking; wrMTrck plugin; ImageJ
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MDPI and ACS Style

Sharma, P.; Lam, V.K.; Raub, C.B.; Chung, B.M. Tracking Single Cells Motility on Different Substrates. Methods Protoc. 2020, 3, 56. https://doi.org/10.3390/mps3030056

AMA Style

Sharma P, Lam VK, Raub CB, Chung BM. Tracking Single Cells Motility on Different Substrates. Methods and Protocols. 2020; 3(3):56. https://doi.org/10.3390/mps3030056

Chicago/Turabian Style

Sharma, Pooja; Lam, Van K.; Raub, Christopher B.; Chung, Byung M. 2020. "Tracking Single Cells Motility on Different Substrates" Methods Protoc. 3, no. 3: 56. https://doi.org/10.3390/mps3030056

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