Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.1.1. Chemicals
2.1.2. Cells
2.1.3. Equipment
2.2. Methods
2.2.1. Absorbance Spectra
2.2.2. Intrinsic Emission Fluorescence by Fluorescence Spectrometry
2.2.3. Dose-Response Curve Based on MTT
3. Results
3.1. Absorbance Spectra
3.2. Dose-Response Curve Based on MTT
4. Discussion and Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Luis, C.; Castaño-Guerrero, Y.; Soares, R.; Sales, G.; Fernandes, R. Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line. Methods Protoc. 2019, 2, 29. https://doi.org/10.3390/mps2020029
Luis C, Castaño-Guerrero Y, Soares R, Sales G, Fernandes R. Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line. Methods and Protocols. 2019; 2(2):29. https://doi.org/10.3390/mps2020029
Chicago/Turabian StyleLuis, Carla, Yuselis Castaño-Guerrero, Raquel Soares, Goreti Sales, and Rúben Fernandes. 2019. "Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line" Methods and Protocols 2, no. 2: 29. https://doi.org/10.3390/mps2020029