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Article

Co-Registration of Bioluminescence Tomography, Computed Tomography, and Magnetic Resonance Imaging for Multimodal In Vivo Stem Cell Tracking

by 1,2,3, 1,2 and 1,2,3,4,5,*
1
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
2
Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
3
Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
4
Department of Chemical & Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
5
Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
*
Author to whom correspondence should be addressed.
Tomography 2016, 2(2), 158-165; https://doi.org/10.18383/j.tom.2016.00160
Received: 3 March 2016 / Revised: 9 April 2016 / Accepted: 11 May 2016 / Published: 1 June 2016
We present a practical approach for coregistration of bioluminescence tomography (BLT), computed tomography (CT), and magnetic resonance (MR) images. For this, we developed a customized animal shuttle composed of nonfluorescent, MR-compatible Delrin plastic that fits a commercially available MR surface coil. Mouse embryonic stem cells were transfected with the luciferase gene and labeled with superparamagnetic iron oxide nanoparticles. Cells were stereotaxically implanted in the mouse brain and imaged weekly for 4 weeks with bioluminescent imaging (IVIS Spectrum CT scanner) and magnetic resonance imaging (MRI; 11.7 T horizontal bore scanner). Without the use of software coregistration, in vitro phantom studies yielded root-mean-square errors of 7.6 × 10−3, 0.93 mm, and 0.78 mm along the medial–lateral (ML), dorsal–ventral (DV), and anterior–posterior (AP) axes, respectively. Rotation errors were negligible. Software coregistration by translation along the DV and AP axes resulted in consistent agreement between the CT and MR images, without the need for rotation or warping. In vivo coregistered BLT/MRI mouse brain data sets showed a single diffuse region of bioluminescent imaging photon signal and MRI hypointensity. Over time, the transplanted cells formed tumors as histopathologically validated. Disagreement between BLT and MRI tumor location was greatest along the DV axis (1.4 ± 0.2 mm) than along the ML (0.5 ± 0.3 mm) and the AP axes (0.6 mm) because of the uncertainty of the depth of origin of the BLT signal. Combining the high spatial anatomical information of MRI with the cell viability/proliferation data from BLT should facilitate preclinical evaluation of novel therapeutic candidate stem cells.
Keywords: multimodal imaging; stem cells; cell tracking; computed tomography; bioluminescence imaging; magnetic resonance imaging; coregistration multimodal imaging; stem cells; cell tracking; computed tomography; bioluminescence imaging; magnetic resonance imaging; coregistration
MDPI and ACS Style

Chehade, M.; Srivastava, A.K.; Bulte, J.W.M. Co-Registration of Bioluminescence Tomography, Computed Tomography, and Magnetic Resonance Imaging for Multimodal In Vivo Stem Cell Tracking. Tomography 2016, 2, 158-165. https://doi.org/10.18383/j.tom.2016.00160

AMA Style

Chehade M, Srivastava AK, Bulte JWM. Co-Registration of Bioluminescence Tomography, Computed Tomography, and Magnetic Resonance Imaging for Multimodal In Vivo Stem Cell Tracking. Tomography. 2016; 2(2):158-165. https://doi.org/10.18383/j.tom.2016.00160

Chicago/Turabian Style

Chehade, Moussa, Amit K. Srivastava, and Jeff W. M. Bulte 2016. "Co-Registration of Bioluminescence Tomography, Computed Tomography, and Magnetic Resonance Imaging for Multimodal In Vivo Stem Cell Tracking" Tomography 2, no. 2: 158-165. https://doi.org/10.18383/j.tom.2016.00160

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