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Tomography
Volume 2
Issue 1
10.18383/j.tom.2016.00106
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Tomography is published by MDPI from Volume 7 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Grapho, LLC.
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Article

Non-Invasive Differentiation of Benign Renal Tumors from Clear Cell Renal Cell Carcinomas Using Clinically Translatable Hyperpolarized 13C Pyruvate Magnetic Resonance

by
Renuka Sriram
1,
Mark Van Criekinge
1,
Justin DeLos Santos
1,
Kayvan R. Keshari
2,
David M. Wilson
1,
Donna Peehl
3,
John Kurhanewicz
1 and
Zhen J. Wang
1,*
1
Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, USA
2
Radiology and Molecular Pharmacology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
3
Department of Urology, Stanford University, Stanford, CA, USA
*
Author to whom correspondence should be addressed.
Tomography 2016, 2(1), 35-42; https://doi.org/10.18383/j.tom.2016.00106
Submission received: 3 December 2015 / Revised: 7 January 2016 / Accepted: 11 February 2016 / Published: 1 March 2016
Versions Notes

Abstract

Incidental detection of localized renal tumors at imaging is increasing. Conventional imaging cannot reliably differentiate the 20% of these tumors that are benign from malignant renal cell carcinomas (RCCs), leading to unnecessary surgical resection and resulting morbidity. Here, we investigated hyperpolarized 13C pyruvate metabolism in live patient-derived renal tumor tissue slices using a novel magnetic resonance-compatible bioreactor platform. We show, for the first time, that clear cell RCCs (ccRCCs), which constitute 70%–80% of all RCCs, exhibit increased lactate production and rapid lactate efflux when compared with benign renal tumors. This difference is because of increased lactate dehydrogenase A and monocarboxylate transporter 4 expression in ccRCCs. Thus, RCCs can be differentiated from benign renal tumors by assessing this distinctive metabolic phenotype using clinically translatable hyperpolarized 13C pyruvate magnetic resonance.
Keywords: hyperpolarized 13C magnetic resonance; dynamic nuclear polarization; aerobic glycolysis; lactate efflux; renal cell carcinoma; patient-derived tissue slice cultures hyperpolarized 13C magnetic resonance; dynamic nuclear polarization; aerobic glycolysis; lactate efflux; renal cell carcinoma; patient-derived tissue slice cultures

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MDPI and ACS Style

Sriram, R.; Van Criekinge, M.; Santos, J.D.; Keshari, K.R.; Wilson, D.M.; Peehl, D.; Kurhanewicz, J.; Wang, Z.J. Non-Invasive Differentiation of Benign Renal Tumors from Clear Cell Renal Cell Carcinomas Using Clinically Translatable Hyperpolarized 13C Pyruvate Magnetic Resonance. Tomography 2016, 2, 35-42. https://doi.org/10.18383/j.tom.2016.00106

AMA Style

Sriram R, Van Criekinge M, Santos JD, Keshari KR, Wilson DM, Peehl D, Kurhanewicz J, Wang ZJ. Non-Invasive Differentiation of Benign Renal Tumors from Clear Cell Renal Cell Carcinomas Using Clinically Translatable Hyperpolarized 13C Pyruvate Magnetic Resonance. Tomography. 2016; 2(1):35-42. https://doi.org/10.18383/j.tom.2016.00106

Chicago/Turabian Style

Sriram, Renuka, Mark Van Criekinge, Justin DeLos Santos, Kayvan R. Keshari, David M. Wilson, Donna Peehl, John Kurhanewicz, and Zhen J. Wang. 2016. "Non-Invasive Differentiation of Benign Renal Tumors from Clear Cell Renal Cell Carcinomas Using Clinically Translatable Hyperpolarized 13C Pyruvate Magnetic Resonance" Tomography 2, no. 1: 35-42. https://doi.org/10.18383/j.tom.2016.00106

APA Style

Sriram, R., Van Criekinge, M., Santos, J. D., Keshari, K. R., Wilson, D. M., Peehl, D., Kurhanewicz, J., & Wang, Z. J. (2016). Non-Invasive Differentiation of Benign Renal Tumors from Clear Cell Renal Cell Carcinomas Using Clinically Translatable Hyperpolarized 13C Pyruvate Magnetic Resonance. Tomography, 2(1), 35-42. https://doi.org/10.18383/j.tom.2016.00106

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