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Tomography
Volume 1
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10.18383/j.tom.2015.00127
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Tomography is published by MDPI from Volume 7 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Grapho, LLC.
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Communication

NeuO for Neuronal Labeling in Zebrafish

by
Chai Lean Teoh
1,
Jun Cheng Er
2,
Parag Mukherjee
3 and
Young-Tae Chang
1,3,*
1
Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore, Singapore
2
Graduate School for Integrative Sciences and Engineering, Centre for LifeSciences, National University of Singapore, Singapore, Singapore
3
Department of Chemistry and MedChem Program of Life Sciences Institute, National University of Singapore, Singapore, Singapore
*
Author to whom correspondence should be addressed.
Tomography 2015, 1(1), 30-36; https://doi.org/10.18383/j.tom.2015.00127
Submission received: 3 June 2015 / Revised: 8 July 2015 / Accepted: 4 August 2015 / Published: 1 September 2015
Versions Notes

Abstract

We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.
Keywords: BODIPY; fluorescent probes; imaging agents; live neurons; zebrafish BODIPY; fluorescent probes; imaging agents; live neurons; zebrafish

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MDPI and ACS Style

Teoh, C.L.; Er, J.C.; Mukherjee, P.; Chang, Y.-T. NeuO for Neuronal Labeling in Zebrafish. Tomography 2015, 1, 30-36. https://doi.org/10.18383/j.tom.2015.00127

AMA Style

Teoh CL, Er JC, Mukherjee P, Chang Y-T. NeuO for Neuronal Labeling in Zebrafish. Tomography. 2015; 1(1):30-36. https://doi.org/10.18383/j.tom.2015.00127

Chicago/Turabian Style

Teoh, Chai Lean, Jun Cheng Er, Parag Mukherjee, and Young-Tae Chang. 2015. "NeuO for Neuronal Labeling in Zebrafish" Tomography 1, no. 1: 30-36. https://doi.org/10.18383/j.tom.2015.00127

APA Style

Teoh, C. L., Er, J. C., Mukherjee, P., & Chang, Y.-T. (2015). NeuO for Neuronal Labeling in Zebrafish. Tomography, 1(1), 30-36. https://doi.org/10.18383/j.tom.2015.00127

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