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Bioengineering 2018, 5(2), 48; https://doi.org/10.3390/bioengineering5020048

Dynamic Cultivation of Mesenchymal Stem Cell Aggregates

1
Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
2
Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Danube University Krems, Dr.-Karl-Dorrek-Straße 30, 3500 Krems, Austria
3
Division of Oncology, Department of Medical and Surgical Sciences for Children & Adults, University-Hospital of Modena and Reggio Emilia, Via Università 4, 41121 Modena, Italy
4
Technopole of Mirandola TPM, 41037 Mirandola, Modena, Italy
*
Author to whom correspondence should be addressed.
Received: 2 May 2018 / Revised: 24 May 2018 / Accepted: 15 June 2018 / Published: 19 June 2018
(This article belongs to the Special Issue Advanced Dynamic Cell and Tissue Culture)
Full-Text   |   PDF [997 KB, uploaded 19 June 2018]   |  

Abstract

Mesenchymal stem cells (MSCs) are considered as primary candidates for cell-based therapies due to their multiple effects in regenerative medicine. Pre-conditioning of MSCs under physiological conditions—such as hypoxia, three-dimensional environments, and dynamic cultivation—prior to transplantation proved to optimize their therapeutic efficiency. When cultivated as three-dimensional aggregates or spheroids, MSCs display increased angiogenic, anti-inflammatory, and immunomodulatory effects as well as improved stemness and survival rates after transplantation, and cultivation under dynamic conditions can increase their viability, proliferation, and paracrine effects, alike. Only few studies reported to date, however, have utilized dynamic conditions for three-dimensional aggregate cultivation of MSCs. Still, the integration of dynamic bioreactor systems, such as spinner flasks or stirred tank reactors might pave the way for a robust, scalable bulk expansion of MSC aggregates or MSC-derived extracellular vesicles. This review summarizes recent insights into the therapeutic potential of MSC aggregate cultivation and focuses on dynamic generation and cultivation techniques of MSC aggregates. View Full-Text
Keywords: mesenchymal stem cells; aggregates; spheroids; dynamic cultivation; bioreactor cultivation; extracellular vesicles; therapeutic potential; scaffold-free mesenchymal stem cells; aggregates; spheroids; dynamic cultivation; bioreactor cultivation; extracellular vesicles; therapeutic potential; scaffold-free
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Egger, D.; Tripisciano, C.; Weber, V.; Dominici, M.; Kasper, C. Dynamic Cultivation of Mesenchymal Stem Cell Aggregates. Bioengineering 2018, 5, 48.

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