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Bioengineering 2018, 5(2), 29; https://doi.org/10.3390/bioengineering5020029

A 3D Microfluidic Model to Recapitulate Cancer Cell Migration and Invasion

1,2
,
2,3
,
2,4,5,6,7,8,9
and
10,11,*
1
Department of Biomedical Engineering, 4 Engineering Drive, National University of Singapore, Singapore 117853, Singapore
2
Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore
3
Integrated Health Information Systems (IHiS), 6 Serangoon North Avenue 5, Singapore 554910, Singapore
4
Department of Physiology, Yong Loo Lin School of Medicine, MD9-04-11, 2 Medical Drive, Singapore 117597, Singapore
5
Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411, Singapore
6
Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #10-01 CREATE Tower, Singapore 138602, Singapore
7
NUS Graduate Programme in Bioengineering, NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117597, Singapore
8
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
9
Gastroenterology Department, Southern Medical University, Guangzhou 510515, China
10
Division of Biotechnology, IFM, Linköping University, Linköping 58183, Sweden
11
Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Korea
*
Author to whom correspondence should be addressed.
Received: 14 March 2018 / Revised: 3 April 2018 / Accepted: 4 April 2018 / Published: 8 April 2018
(This article belongs to the Special Issue Advances in Micro-Bioreactor Design for Organ Cell Studies)
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Abstract

We have developed a microfluidic-based culture chip to simulate cancer cell migration and invasion across the basement membrane. In this microfluidic chip, a 3D microenvironment is engineered to culture metastatic breast cancer cells (MX1) in a 3D tumor model. A chemo-attractant was incorporated to stimulate motility across the membrane. We validated the usefulness of the chip by tracking the motilities of the cancer cells in the system, showing them to be migrating or invading (akin to metastasis). It is shown that our system can monitor cell migration in real time, as compare to Boyden chambers, for example. Thus, the chip will be of interest to the drug-screening community as it can potentially be used to monitor the behavior of cancer cell motility, and, therefore, metastasis, in the presence of anti-cancer drugs. View Full-Text
Keywords: 3D cell culture; microfluidics; cell migration; cell invasion; metastasis 3D cell culture; microfluidics; cell migration; cell invasion; metastasis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Toh, Y.-C.; Raja, A.; Yu, H.; van Noort, D. A 3D Microfluidic Model to Recapitulate Cancer Cell Migration and Invasion. Bioengineering 2018, 5, 29.

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