1. Introduction
In the real world, there are data values that are uncertainly specified in many applications, for instance sensor information. The fuzzy set theory [
1] has been introduced to handle this vagueness by extending the concept of belonging to a set. In a fuzzy set, each element is essentially associated with a point value selected in the range of units [0, 1], indicated as the quality of belonging membership grade of the set. Klement and Mesiar [
2] reviewed some generalizations of fuzzy sets with two- or three-dimensional lattices of truth values and study their relationship. A vague set introduced by Gau and Buehrer [
3] is a further extension of a fuzzy set. Instead of using point-based membership as in fuzzy set, interval-based membership is used in a vague set and to describe the boundaries of the membership degree, a true-membership function and a false membership function are used. Belonging to intervals in vague sets is more expressive to capture data inaccuracy. In literature, the notions of intuitionistic fuzzy set and vague set are considered isomorphic. Deng et al. [
4] presented the distance between vague sets with applications in decision making. Wang [
5] developed vague parameterized vague soft sets and provide its applications in decision-making.
A graph is a useful tool for describing decision problems in a diagram format. Using this tool, decision objects and their relationship are represented by vertices and edges, respectively. Rosenfeld [
6] introduced the fuzzy graphs by providing a fuzzy analogue of several basic graph-theoretic concepts. Ramakrishna [
7] originally developed the concept of vague graphs and discussed their prominent characteristics. The vague models are more and more flexible, and practical as compared to the crisp and fuzzy models. The notion of vague hypergraphs was defined by Akram et al. [
8]. Later, Akram et al. introduced many new concepts under vague environment, such as Cayley vague graphs [
9], types of vague cycles and vague trees [
10], regularity in vague intersection graphs and vague line graphs [
11], and certain types of vague graphs [
12] and so forth. Borzooei and Rashmanlou [
13,
14] investigated domination in vague graphs and degree of vertices in vague graphs. Nowadays, extended fuzzy graph theory is a hot research area. Recently, many researchers discussed the graph theoretical concepts in generalized fuzzy environment, such as Pythagorean fuzzy graphs (PFG) [
15], complex Pythagorean fuzzy graphs [
16], rough fuzzy graphs [
17] and q-rung orthopair fuzzy graphs [
18] along with its decision making applications.
Product operations on graphs play a very important role in graph theory. Many scholars discussed product operations on various generalized fuzzy graphs. Mordeson and Peng [
19] defined some of these product operations on fuzzy graphs. Subsequently, utilizing these operations, the degree of the vertices is obtained from two fuzzy graphs in Reference [
20]. Gong and Wang [
21] have defined some operations on the product fuzzy hypergraphs. Sahoo and Pal [
22] presented some operations on intuitionistic fuzzy graph (IFG) products and calculated the vertex degree in the IFGs. Rashmanlou et al. [
23] proposed product operations over a range of values fuzzy graphs, that is, interval-valued fuzzy graphs and discussed the degree of a vertex in fuzzy graphs with interval values. Recently, Naz et al. [
15] studied some of the PFG operations, the vertex degree and the vertex total degree in the PFG. However, the maximal operations on products with vague graphs were not sought. Therefore, in this paper, we will focus on this topic. We present new product of graphs, namely, maximal product of two vague graphs. Then we describe certain concepts, including strongly, completely, regularity and connectedness on maximal product of vague graphs. Fuhrer, we consider some results of edge regular and totally edge regular in maximal product of vague graphs. Finally, we present an application for optimization of the biomass based on maximal product of vague graphs.
  2. Maximal Products of Vague Graphs
In this section we introduce the concept of maximal product of two vague graphs. Then we get some results on strong and complete maximal product of two vague graphs.
Definition 1 ([
3])
. A vague set A is a pair , where  and  are fuzzy sets on finite non-empty set X and we called them by true and false membership functions, respectively, such that for all , . Definition 2 ([
7])
. Let X and Y be ordinary finite non-empty sets. We call a vague relation to be a vague subset of , that is, an expression R defined by:where , , which satisfies the condition , for all . Definition 3 ([
7])
. A vague graph on a non-empty set V is a pair , where  is a vague set on V and  is a vague relation on V such thatfor all x,  Note that B is called vague relation on A. Definition 4 ([
7])
. Let  be a simple graph and  be a vague graph on . Then a vague graph G is called:- Strong if for every edge, 
- Complete vague graph, if for every, 
 Definition 5. Letandbe two vague graphs on simple graphsand, respectively. Define, whereandand we define vague graphon, for allbyand for allby Thenis called the maximal product of two vague graphsandand denoted by,and.
 Example 1. Consider the vague graphsandas in Figure 1. In the right side of following figures, we can see that the maximal product of two vague graphsand, that is.  Proposition 1. Maximal product of two vague graphsand, is a vague graph.
 Proof.  Let  and  be two vague graphs on simple graphs  and , respectively. Then by Definition 5, we have two cases:
Case (i): If 
 and 
, then we have
        
        and
        
        Hence in this case we have
        
        Case (ii): The proof is similar to the proof of case (i), by the some modification.
Therefore,  is a vague graph. □
 Theorem 1. The maximal product of two strong vague graphs is a strong vague graphs.
 Proof.  Let  and  be two strong vague graphs on simple graphs  and , respectively, and  be the maximal product of  and . Then by Proposition 1, G is a vague graph. Now we have two cases:
Case (i): If 
 and 
, then we have
        
        by the similar way 
.
Case (ii): The proof is similar to the proof of case (i), by some modifications.
Thus for all edge in the maximal product 
, we have
        
        and
        
        Hence 
G is a strong vague graph. □
 Remark 1. If maximal product of two vague graphsandis a strong vague graph, thenandneed not to be strong vague graphs, in general.
 Example 2. Consider the vague graphs,and, by the Figure 2. Thenand G are strong vague graphs, butis not a strong vague graph. Since,, but. Hence.
 Theorem 2. The maximal product of two connected vague graphs is a connected vague graph.
 Proof.  Let  and  be two connected vague graphs on simple graphs  and , respectively, where  and . Then , for all  and , for all  (or , for all  and , for all ). The maximal product of  and  can be taken as . Now consider the  subgraphs of G with the vertex sets , for . Each of these subgraphs of G is connected, since the ’s are the same and since  is connected, each  is adjacent to at least one of the vertices in . Also since  is connected, each  is adjacent to at least one of the vertices in . Hence there exists at least one edge between any pair of the above  subgraphs. Thus we have  (or ), for all . Hence, G is a connected vague graph. □
 Remark 2. Maximal product of two complete vague graphs is not a complete vague graphs, in general. Because we do not include the caseandin the definition of the maximal product of vague graphs. Since every complete vague graph is strong, we have the maximal product of two complete vague graph is a strong vague graph.
 Definition 6. Letandbe two vague graphs on simple graphsand, respectively, whereand,. The degree of any vertex in the maximal product of the vague graphsandis defined by,, where  Example 3. Consider the vague graphs in Example 2. Then  Definition 7. Letandbe two vague graphs on simple graphsand, respectively. Then, the maximal product ofand, that is, is called-regular if, for all. In this case, G is called regular vague graph (of degree).
 Example 4. Consider two vague graphsandand maximal product vague graphas in Figure 3. Henceis a regular vague graph of degree.
 Note. If  and  are two regular vague graphs, then the maximal product of  and  is not a regular vague graph, in general.
Example 5. Consider regular vague graphsandand maximal product vague graphas in Figure 4. Since, but. Thenis not regular.
 Definition 8. Letbe a vague graph on simple graph. Ifis a regular graph, then G is said to be partially regular vague graph. If G is both regular and partially regular vague graph, then G is said to be a full regular vague graph.
 Example 6. Consider the vague graphs,andas Example 4. Sinceis a regular graph, so G is a partially regular vague graph. Hence G is a full regular vague graph.
 Definition 9. Let G be the maximal product of two vague graphsand. Then the degree of an edgeis defined aswhereMoreover, the total degree of an edgeis defined aswhere  Example 7. Consider the vague graphs,and the maximal productin Example 5. Then  Theorem 3. Letandbe two vague graphs andbe the finite underlying crisp graph of, which is a cycle. Then the summation of degree of all vertices inis equal to the summation of degree of all edges in, that is:where.  Proof.  Let 
 be a vague graph and 
 be the simple graph of 
G which is a cycle, that is 
 for all 
, where 
. Then
        
        Now we have
        
      
        
      
      
      
      
     
        Similarly,
        
        Hence,
        
		 □
 Remark 3. Letandbe two vague graphs andwith simple graph. Thenwherefor all.  Definition 10. Letandbe a vague graphs and. If each edge in vague graphhas the same degree, thenis said to be an-edge regular vague graph. Moreover if each edge in vague graphhas the same total degree, thenis said to be a-totally edge regular vague graph.
 Example 8. Consider the vague graph,and G as in Example 4. Then G is an-edge regular vague graph and G is a-totally edge regular.
 Example 9. Consider the vague graphas in Figure 5. ThenSois an-edge regular vague graph, but is not an totally edge regular vague graph.  Theorem 4. Letandbe two vague graphs,andbe the simple graph of G. Ifis k-regular, then the summation of degree of all edges in G, is as follows:  Proof.  By Remark 3, we have
        
      
        
      
      
      
      
     
        Since 
 is a regular crisp graph, 
, for all 
 and so we have
        
        Hence
        
		So
        
		 □
 Theorem 5. Letandbe two vague graphs,andbe the simple graph of G. Then the summation of total degree of all edges in G is as follows:  Proof.  By definition of total edge degree of 
G, we have
        
      
        
      
      
      
      
     
        By Remark 3, we get
        
		 □
 Theorem 6. Letandbe two vague graphs on simple graphsand, respectively./ Thenof
is a constant function if and only if the following are equivalent:
- (i)
- G is an edge regular vague graph. 
- (ii)
- G is a totally edge regular vague graph. 
 Proof.  Assume that 
 of 
 is a constant function. Then
          
            for every 
, where 
 and 
 are constants. Let 
G be an 
-edge regular vague graph. Then, for all 
,
 and
          
           for all 
. Then 
G is a totally edge regular. Now, let 
G be a 
-totally edge regular vague graph. Then 
, for all 
. So we have
          
		  Hence
          
		  Then 
G is a 
 edge regular vague graph.
Conversely, assume that (i) and (ii) are equivalent. We have to prove that 
 is a constant function. Suppose that 
 is not a constant function. Then 
 and 
, for at least one pair of 
. Let 
G be an 
 edge regular vague graph. Then
          
		  Hence for all 
 and for all 
,
          
		  and
          
		  Since
          
		  and
          
		  we have
          
		  Hence 
G is not a totally edge regular, that is a contradiction to our assumption. Therefore 
 is a constant function. Similarly, we can show that 
 is a constant function, when 
G is a totally edge regular vague graph. □
   3. Application
In this section, we express application of maximal product of two vague graphs. To this end we want to optimize the production of bacterial biomass. One of the most commonly used bacteria in genetic engineering is 
Escherichia coli. Many high-efficiency proteins are expressed in Escherichia coli using genetic engineering technologies. The recombinant protein is a protein that has been added to the genetic engineering by virtue of its genetic engineering. For this purpose, in order to produce recombinant proteins in the research laboratory, bacteria are grown in liquid medium containing essential nutrients. 
E. Coli is able to produce a large amount of biomass and consequently, increase the production of the desired protein in a nutrient-rich medium. It should be noted that biomass is, in fact, a degradable source composed of living organisms. Cultivation conditions are divided into two biological and physical categories. Biological sources for bacterial growth medium include carbon source, nitrogen source and Percentage of inoculum pre-culture. Physical conditions set by the device include Potential of hydrogen, temperature, dissolved oxygens, revolutions per minute and finally, the optimization of bacterial culture conditions leads to maximizing the production efficiency of large quantities of the product (recombinant proteins), which ultimately results in increased economic efficiency. According to studies by Hosseini et al. [
24], the optimal conditions for bacterial cultivation were reported as follows: due to the differences in these parameters, the optical absorption of bacterial culture medium at a wavelength of 600 nm is considered to be consistent with all the parameters. Our goal is to model this problem using vague graphs. Now in 
Figure 6, we assume 
 is the vague graph of the physical conditions and 
 is the vague graph of the biological conditions where 
potential of hidrogen, temperature, dissolved oxygene, revolutions per minute} as shown in 
Table 1, and 
carbon source, nitrogen source, percentage of inoculum pre-culture}. Also 
 is the intensity of optical absorption of the optimum conditions, 
 the intensity of optical absorption under common conditions, 
 is the term of the mass in grams in optimal conditions and 
 in terms of mass gram in the optimal. By direct calculations, degree of vertices in vague graphs 
 and 
 are given in 
Table 2 and 
Table 3, and degree of edges in vague graphs 
 and 
 are given in 
Table 4 and 
Table 5.
Now we consider the vague graph 
 as follows (see 
Table 6 and 
Table 7):
The results of the 
Table 6 are matched with the results of experiments. So that, due to the increasing growth of bacteria, in the presence of optimized sources of 
, 
 and 
 intensity of absorption measured by the spectrophotometer is the highest number in optimal condition and lowest number in optical ordinary condition. Based on the results of 
Table 6, the high values of obtained biomass from simultaneous use of optimal nitrogen source, optimal carbon source, Percentage of inoculum pre-culture and optimal dissolved oxygen in erlenmeyer in laboratory condition match with the obtained biomass using of maximal product 
. Although, in this case, low values of biomass in common condition have the same terms of mass gram in both laboratory condition and 
Table 6. So that by 
Table 7, the biomass obtained from 
, 
 and 
 in optimal conditions, compared to all other investigated optimal mode, have the highest terms of mass gram. Likewise biomass obtained from 
, 
 and 
 in common conditions, compared to all other investigated optimal mode, have the lowest terms of mass gram. Hence the results of 
Table 7 are matched with the results of experiments. Vague graph 
 is shown in 
Figure 7.
Therefore, we conclude that the mathematical modeling of this laboratory research using a maximized multiplication graph is a good tool for predicting laboratory samples for optimal biomass production and a suitable link between biotechnology and fuzzy mathematics can be established.