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Volume 5
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10.11599/germs.2015.1066
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GERMS is published by MDPI from Volume 25 Issue 4 (2025). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with the former publisher Infection Science Forum S.R.L..
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Article

Nocardia Isolation from Clinical Samples with the Paraffin Baiting Technique

by
Mehdi Fatahi Bafghi
1,
Parvin Heidarieh
2,
Tahereh Soori
3,
Sasan Saber
4,
Alipasha Meysamie
5,
Khavar Gheitoli
4,
Shadi Habibnia
1,
Masoumeh Rasouli Nasab
1 and
Seyyed Saeed Eshraghi
1,*
1
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2
Department of Bacteriology and Virology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
3
Department of Infectious Diseases, Razi hospital, Tehran University of Medical Sciences, Tehran, Iran
4
Department of Pulmonary Infection, Doctor Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
5
Department of Community Medicine, Tehran University of Medical Sciences, Tehran, Iran
*
Author to whom correspondence should be addressed.
GERMS 2015, 5(1), 12-16; https://doi.org/10.11599/germs.2015.1066
Submission received: 2 December 2014 / Revised: 18 January 2015 / Accepted: 17 February 2015 / Published: 2 March 2015
Versions Notes

Abstract

Background: The genus Nocardia is a cause of infection in the lungs, skin, brain, cerebrospinal fluid, eyes, joints and kidneys. Nocardia isolation from polymicrobial specimens is difficult due to its slow growth. Several methods have been reported for Nocardia isolation from clinical samples. In the current study, we used three methods: paraffin baiting technique, paraffin agar, and conventional media for Nocardia isolation from various clinical specimens from Iranian patients. Methods: In this study, we examined 517 samples from various clinical specimens such as: sputum of patients with suspected tuberculosis, bronchoalveolar lavage, sputum of patients with cystic fibrosis, tracheal aspirate, cutaneous and subcutaneous abscesses, cerebrospinal fluid, dental abscess, mycetoma, wound, bone marrow biopsy, and gastric lavage. All collected specimens were cultured on carbon-free broth tubes (paraffin baiting technique), paraffin agar, Sabouraud dextrose agar, and Sabouraud dextrose agar with cycloheximide and were incubated at 35°C for one month. Results: Seven Nocardia spp. were isolated with paraffin baiting technique, compared with 5 positive results with the paraffin agar technique and 3 positive results with Sabouraud dextrose agar with and without cycloheximide. The prevalence of nocardial infections in our specimens was 5.28%. Conclusion: In the present study, the use of the paraffin baiting technique appeared to be more effective than other methods for Nocardia isolation from various clinical specimens.
Keywords: Nocardia; paraffin baiting technique; paraffin agar; Sabouraud dextrose agar Nocardia; paraffin baiting technique; paraffin agar; Sabouraud dextrose agar

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MDPI and ACS Style

Bafghi, M.F.; Heidarieh, P.; Soori, T.; Saber, S.; Meysamie, A.; Gheitoli, K.; Habibnia, S.; Nasab, M.R.; Eshraghi, S.S. Nocardia Isolation from Clinical Samples with the Paraffin Baiting Technique. GERMS 2015, 5, 12-16. https://doi.org/10.11599/germs.2015.1066

AMA Style

Bafghi MF, Heidarieh P, Soori T, Saber S, Meysamie A, Gheitoli K, Habibnia S, Nasab MR, Eshraghi SS. Nocardia Isolation from Clinical Samples with the Paraffin Baiting Technique. GERMS. 2015; 5(1):12-16. https://doi.org/10.11599/germs.2015.1066

Chicago/Turabian Style

Bafghi, Mehdi Fatahi, Parvin Heidarieh, Tahereh Soori, Sasan Saber, Alipasha Meysamie, Khavar Gheitoli, Shadi Habibnia, Masoumeh Rasouli Nasab, and Seyyed Saeed Eshraghi. 2015. "Nocardia Isolation from Clinical Samples with the Paraffin Baiting Technique" GERMS 5, no. 1: 12-16. https://doi.org/10.11599/germs.2015.1066

APA Style

Bafghi, M. F., Heidarieh, P., Soori, T., Saber, S., Meysamie, A., Gheitoli, K., Habibnia, S., Nasab, M. R., & Eshraghi, S. S. (2015). Nocardia Isolation from Clinical Samples with the Paraffin Baiting Technique. GERMS, 5(1), 12-16. https://doi.org/10.11599/germs.2015.1066

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