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Article

Antibiotic Resistance Pattern of Pseudomonas spp. from Patients in a Tertiary Hospital in South-West Nigeria

by
Adesola Adejobi
1,
Olabisi Ojo
2,
Olubunmi Alaka
3,
Babatunde Odetoyin
1 and
Anthony Onipede
1,3,*
1
Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, PMB 13, Ile-Ife, Nigeria
2
Department of Natural Sciences, Albany State University, Albany, GA 31705, USA
3
Department of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospitals Complex, PMB 5538, Ile-Ife, Nigeria
*
Author to whom correspondence should be addressed.
GERMS 2021, 11(2), 238-245; https://doi.org/10.18683/germs.2021.1260
Submission received: 24 September 2020 / Revised: 26 May 2021 / Accepted: 31 May 2021 / Published: 2 June 2021

Abstract

Introduction: Pseudomonads constitute critical agents of opportunistic infections in hospital settings particularly in immunocompromised patients and Pseudomonas aeruginosa is a major flagship member of these infectious agents. This study assessed the distribution of Pseudomonas spp. associated with infections in patients and their antibiotic resistance patterns as part of an antibiotic stewardship intervention program and resistance surveillance. Methods: One hundred and fifty Pseudomonas spp. from different clinical specimens were obtained from the Obafemi Awolowo University Teaching Hospitals Complex Ile-Ife. Culture was carried out on MacConkey and blood agar while phenotypic characterization was done by Gram staining, oxidase, and catalase test. Species identification was done using MICROBACTTM 24E bacterial identification kit and confirmed by 16S rDNA polymerase chain reaction (PCR) assay. Antibiotic susceptibility testing to eight antibiotics in four classes was done. Results: Pseudomonas aeruginosa was the most frequently occurring species (96.0%); P. putida (2.67%) and P. fluorescens (0.67%) were also identified as well as an isolate of Burkholderia pseudomallei (0.67%). The highest resistance rate among isolates was observed towards gentamicin (35.4%); piperacillin/tazobactam was the most active antibiotic. Multidrug-resistant (MDR) strains constituted 12.8% of the isolates and most MDR strains also displayed a high multiple antibiotic resistance index (MAR). Conclusions: Pseudomonas aeruginosa is emerging as a highly MDR pathogen in our hospital setting. This calls for the establishment of a surveillance system and antimicrobial stewardship programme in place. Furthermore, we propose a review of the current antibiotics prescription policy, and infection control programmes (ICPs) if we must control the spread of MDR-P. aeruginosa in this environment.
Keywords: Pseudomonas spp.; multiple antibiotic resistance index (MAR); multidrug resistance (MDR); antibiotics stewardship Pseudomonas spp.; multiple antibiotic resistance index (MAR); multidrug resistance (MDR); antibiotics stewardship

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MDPI and ACS Style

Adejobi, A.; Ojo, O.; Alaka, O.; Odetoyin, B.; Onipede, A. Antibiotic Resistance Pattern of Pseudomonas spp. from Patients in a Tertiary Hospital in South-West Nigeria. GERMS 2021, 11, 238-245. https://doi.org/10.18683/germs.2021.1260

AMA Style

Adejobi A, Ojo O, Alaka O, Odetoyin B, Onipede A. Antibiotic Resistance Pattern of Pseudomonas spp. from Patients in a Tertiary Hospital in South-West Nigeria. GERMS. 2021; 11(2):238-245. https://doi.org/10.18683/germs.2021.1260

Chicago/Turabian Style

Adejobi, Adesola, Olabisi Ojo, Olubunmi Alaka, Babatunde Odetoyin, and Anthony Onipede. 2021. "Antibiotic Resistance Pattern of Pseudomonas spp. from Patients in a Tertiary Hospital in South-West Nigeria" GERMS 11, no. 2: 238-245. https://doi.org/10.18683/germs.2021.1260

APA Style

Adejobi, A., Ojo, O., Alaka, O., Odetoyin, B., & Onipede, A. (2021). Antibiotic Resistance Pattern of Pseudomonas spp. from Patients in a Tertiary Hospital in South-West Nigeria. GERMS, 11(2), 238-245. https://doi.org/10.18683/germs.2021.1260

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