Evaluation of Polyphenol Profile from Citrus Peel Obtained by Natural Deep Eutectic Solvent/Ultrasound Extraction

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The manuscript overall is well written and has good analysis. Here are some comments for improvement:

 

1. Lines 76–81

   - : There are already numerous studies on NADES for this particular application. Please consider revising this section to reflect the current state of research and explore any gaps or novel approaches.

 

2. Lines 112–114

   - The combination of NADES and UAE does not represent a particularly innovative approach. Please reconsider this and provide a clearer justification for the method selected.

 

3. Introduction

   -: Please define the objectives of the study more clearly in the introduction to guide the reader through the research focus.

 

4. Line 146

   : Could you specify if any software was used during the analysis or experimentation? If so, please include the details.

 

5. Table 1

   - It is recommended to use "actual" instead of "real" when describing measurements or data points for better clarity and precision.

 

6. Line 240 and Other Locations

   - Please ensure that species names are consistently italicized throughout the manuscript, as per the standard conventions.

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Author Response

 "Please see the attachment."

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The objective of this study is to investigate the extraction of polyphenol compounds from orange peel waste through the utilization of a deep eutectic solvent coupled with ultrasound-assisted technology. Recent publications have included reports that demonstrate the utilization of a similar methodology. This deficiency in the manuscript results in a lack of their novelty and reduces reader engagement. Hence, the decision to reject this manuscript must be implemented.

Author Response

 "Please see the attachment."

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

Comments in attached file.

Comments for author File: Comments.pdf

Comments on the Quality of English Language

Quality of English is satisfactory.

Author Response

 "Please see the attachment."

Author Response File: Author Response.pdf

Reviewer 4 Report

Comments and Suggestions for Authors

This paper evaluates the polyphenol profile of citrus peel obtained from natural deep eutectic solvent/ultrasound extraction. Different effects such as molar ratios (MR) of ChCl/Glu, percentage of added water, and citrus peel types are discussed herein. The extract was analyzed, and the antioxidant capacity was measured. By combining NADES and ultrasound-assisted extraction method, highest concentrations of hesperidin, neohesperidin, and total phenolic content (TPC) are achieved using different MR of ChCl/Glu conditions, highlighting the potential health applications for these extracts obtained with NADES. The study is relatively comprehensive. But there are some minor revisions to make to avoid confusion,

 

1.     In the abstract line 27 and 29, instead of stating 100 g peel, the concentrations of neohesperidin and TPC should be stated as 100 g dry mass.

 

 

2.     For Table S1, the authors should explain what “ND” means, and why some data are highlighted as red color.

Author Response

"Please see the attachment." 

Author Response File: Author Response.pdf

Round 2

Reviewer 3 Report

Comments and Suggestions for Authors

The revised manuscript is now improved and the queries raised have been addressed but the sentence on L 268 could be more clearly expressed. 

Comments on the Quality of English Language

Quality of English is satisfactory.