Hydrogen Sulfide Inhibits TMPRSS2 in Human Airway Epithelial Cells: Implications for SARS-CoV-2 Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines
2.2. Primary Epithelial Cells
2.3. In Vitro Cultures with H2S Donors
2.4. Analysis of Gene Expression
2.5. Analysis of Protein Expression
2.6. Immunohistochemistry Analysis
2.7. Statistical Analysis
3. Results
3.1. H2S-Donors Reduce TMPRSS2 Expression on Airway Epithelial Cells Lines
3.2. NaHS Reduces TMPRSS2 Expression on Primary Epithelial Cells of Both Upper and Lower Airways
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pozzi, G.; Masselli, E.; Gobbi, G.; Mirandola, P.; Taborda-Barata, L.; Ampollini, L.; Carbognani, P.; Micheloni, C.; Corazza, F.; Galli, D.; et al. Hydrogen Sulfide Inhibits TMPRSS2 in Human Airway Epithelial Cells: Implications for SARS-CoV-2 Infection. Biomedicines 2021, 9, 1273. https://doi.org/10.3390/biomedicines9091273
Pozzi G, Masselli E, Gobbi G, Mirandola P, Taborda-Barata L, Ampollini L, Carbognani P, Micheloni C, Corazza F, Galli D, et al. Hydrogen Sulfide Inhibits TMPRSS2 in Human Airway Epithelial Cells: Implications for SARS-CoV-2 Infection. Biomedicines. 2021; 9(9):1273. https://doi.org/10.3390/biomedicines9091273
Chicago/Turabian StylePozzi, Giulia, Elena Masselli, Giuliana Gobbi, Prisco Mirandola, Luis Taborda-Barata, Luca Ampollini, Paolo Carbognani, Cristina Micheloni, Francesco Corazza, Daniela Galli, and et al. 2021. "Hydrogen Sulfide Inhibits TMPRSS2 in Human Airway Epithelial Cells: Implications for SARS-CoV-2 Infection" Biomedicines 9, no. 9: 1273. https://doi.org/10.3390/biomedicines9091273