Apolipoprotein Signature of HDL and LDL from Atherosclerotic Patients in Relation with Carotid Plaque Typology: A Preliminary Report
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Lipoproteins Purification
2.3. SDS-PAGE Analysis
2.4. Shotgun Proteomics
2.5. Protein–Protein Interaction (PPI) Network and Gene Ontology (GO) Analysis
3. Results
3.1. Lipoproteins Purification
3.2. Proteomics Characterization of HDL and LDL
3.3. Differential Expression Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameters | “Hard” (n = 31) | “Soft” (n = 44) |
---|---|---|
Age (years) * | 67.1 ± 6.9 | 72.6 ± 8.0 |
Sex ratio, m/f | 22/9 | 34/10 |
BMI * | 25.3 ± 3.4 | 25.9 ± 3.5 |
Triglycerides (mg/dL) * | 116.8 ± 48.2 | 124.1 ± 44.6 |
Total Cholesterol (mg/dL) * | 166.6 ± 41.4 | 181.3 ± 50.5 |
HDL Cholesterol (mg/dL) * | 47.6 ± 14.1 | 42.3 ± 15.8 |
LDL Cholesterol (mg/dL) * | 96.8 ± 29.8 | 112.0 ± 45.0 |
Cholesterol lowering therapy (%) | 23/31 (74.2) | 41/44 (93.2) |
Glycemia (mg/dL) * | 114.4 ± 35.8 | 107.3 ± 20.9 |
HbA1C (%) * | 6.6 ± 1.0 | 6.5 ± 1.0 |
Type 2 Diabetes (%) | 9/31 (29.0) | 19/44 (43.2) |
Glucose lowering Therapy (%) | 9/9 (100) | 12/19 (63.2) |
Systolic blood pressure (mmHg) * | 138.8 ± 8.7 | 129.1 ± 12.6 |
Diastolic blood pressure (mmHg) * | 79.9 ± 8.9 | 72.4 ± 7.3 |
Anti-hypertensive Therapy (%) | 28/31 (90.3) | 35/44 (79.5) |
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Finamore, F.; Nieddu, G.; Rocchiccioli, S.; Spirito, R.; Guarino, A.; Formato, M.; Lepedda, A.J. Apolipoprotein Signature of HDL and LDL from Atherosclerotic Patients in Relation with Carotid Plaque Typology: A Preliminary Report. Biomedicines 2021, 9, 1156. https://doi.org/10.3390/biomedicines9091156
Finamore F, Nieddu G, Rocchiccioli S, Spirito R, Guarino A, Formato M, Lepedda AJ. Apolipoprotein Signature of HDL and LDL from Atherosclerotic Patients in Relation with Carotid Plaque Typology: A Preliminary Report. Biomedicines. 2021; 9(9):1156. https://doi.org/10.3390/biomedicines9091156
Chicago/Turabian StyleFinamore, Francesco, Gabriele Nieddu, Silvia Rocchiccioli, Rita Spirito, Anna Guarino, Marilena Formato, and Antonio Junior Lepedda. 2021. "Apolipoprotein Signature of HDL and LDL from Atherosclerotic Patients in Relation with Carotid Plaque Typology: A Preliminary Report" Biomedicines 9, no. 9: 1156. https://doi.org/10.3390/biomedicines9091156