Salusin-β in Intermediate Dorsal Motor Nucleus of the Vagus Regulates Sympathetic-Parasympathetic Balance and Blood Pressure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. General Procedures
2.3. RSNA Recording
2.4. DMV Microinjection
2.5. Heart Rate Variability (HRV) Analysis
2.6. In-Situ Detection of Superoxide Anions in DMV
2.7. Measurement of Superoxide Anion Level and NAD(P)H Oxidase Activity
2.8. Immunohistochemistry
2.9. Vagotomy and Sinoaortic Denervation (SAD)
2.10. Chemicals and Antibodies
2.11. Statistics
3. Results
3.1. Effects of Salusin-β in Different Zones of DMV
3.2. Dose-Effects and Time-Effects of Salusin-β in iDMV
3.3. Roles of Vagi in the Effects of Salusin-β in the iDMV
3.4. Effects of Anti-Salusin-β IgG and Salusin-β Immunohistochemistry
3.5. Reactive Oxidative Species (ROS) Production Mediates the Effects of Salusin-β in iDMV
3.6. GABAA Receptors in PVN Mediates the Effects of Salusin-β in iDMV
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wu, L.-L.; Bo, J.-H.; Zheng, F.; Zhang, F.; Chen, Q.; Li, Y.-H.; Kang, Y.-M.; Zhu, G.-Q. Salusin-β in Intermediate Dorsal Motor Nucleus of the Vagus Regulates Sympathetic-Parasympathetic Balance and Blood Pressure. Biomedicines 2021, 9, 1118. https://doi.org/10.3390/biomedicines9091118
Wu L-L, Bo J-H, Zheng F, Zhang F, Chen Q, Li Y-H, Kang Y-M, Zhu G-Q. Salusin-β in Intermediate Dorsal Motor Nucleus of the Vagus Regulates Sympathetic-Parasympathetic Balance and Blood Pressure. Biomedicines. 2021; 9(9):1118. https://doi.org/10.3390/biomedicines9091118
Chicago/Turabian StyleWu, Lu-Lu, Jin-Hua Bo, Fen Zheng, Feng Zhang, Qi Chen, Yue-Hua Li, Yu-Ming Kang, and Guo-Qing Zhu. 2021. "Salusin-β in Intermediate Dorsal Motor Nucleus of the Vagus Regulates Sympathetic-Parasympathetic Balance and Blood Pressure" Biomedicines 9, no. 9: 1118. https://doi.org/10.3390/biomedicines9091118