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Article

Microbiota Depletion Promotes Human Rotavirus Replication in an Adult Mouse Model

1
Department of Microbiology, School of Medicine, University of Valencia, Av. Blasco Ibáñez 17, 46010 Valencia, Spain
2
Hospital Clínico Universitario de Valencia, Instituto de Investigación INCLIVA, 46010 Valencia, Spain
3
Department of Biotechnology, IATA-CSIC, Av. Agustín Escardino 7, Paterna, 46980 Valencia, Spain
*
Authors to whom correspondence should be addressed.
These two authors contributed equally.
Academic Editors: Federica Laudisi and Carmine Stolfi
Biomedicines 2021, 9(7), 846; https://doi.org/10.3390/biomedicines9070846
Received: 23 June 2021 / Revised: 7 July 2021 / Accepted: 15 July 2021 / Published: 20 July 2021
(This article belongs to the Special Issue Gut Dysbiosis: Molecular Mechanisms and Therapies)
Intestinal microbiota-virus-host interaction has emerged as a key factor in mediating enteric virus pathogenicity. With the aim of analyzing whether human gut bacteria improve the inefficient replication of human rotavirus in mice, we performed fecal microbiota transplant (FMT) with healthy infants as donors in antibiotic-treated mice. We showed that a simple antibiotic treatment, irrespective of FMT, resulted in viral shedding for 6 days after challenge with the human rotavirus G1P[8] genotype Wa strain (RVwa). Rotavirus titers in feces were also significantly higher in antibiotic-treated animals with or without FMT but they were decreased in animals subject to self-FMT, where a partial re-establishment of specific bacterial taxons was evidenced. Microbial composition analysis revealed profound changes in the intestinal microbiota of antibiotic-treated animals, whereas some bacterial groups, including members of Lactobacillus, Bilophila, Mucispirillum, and Oscillospira, recovered after self-FMT. In antibiotic-treated and FMT animals where the virus replicated more efficiently, differences were observed in gene expression of immune mediators, such as IL1β and CXCL15, as well as in the fucosyltransferase FUT2, responsible for H-type antigen synthesis in the small intestine. Collectively, our results suggest that antibiotic-induced microbiota depletion eradicates the microbial taxa that restrict human rotavirus infectivity in mice. View Full-Text
Keywords: rotavirus; antibiotic; microbiota; mice; virus shedding rotavirus; antibiotic; microbiota; mice; virus shedding
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MDPI and ACS Style

Gozalbo-Rovira, R.; Santiso-Bellón, C.; Buesa, J.; Rubio-del-Campo, A.; Vila-Vicent, S.; Muñoz, C.; Yebra, M.J.; Monedero, V.; Rodríguez-Díaz, J. Microbiota Depletion Promotes Human Rotavirus Replication in an Adult Mouse Model. Biomedicines 2021, 9, 846. https://doi.org/10.3390/biomedicines9070846

AMA Style

Gozalbo-Rovira R, Santiso-Bellón C, Buesa J, Rubio-del-Campo A, Vila-Vicent S, Muñoz C, Yebra MJ, Monedero V, Rodríguez-Díaz J. Microbiota Depletion Promotes Human Rotavirus Replication in an Adult Mouse Model. Biomedicines. 2021; 9(7):846. https://doi.org/10.3390/biomedicines9070846

Chicago/Turabian Style

Gozalbo-Rovira, Roberto, Cristina Santiso-Bellón, Javier Buesa, Antonio Rubio-del-Campo, Susana Vila-Vicent, Carlos Muñoz, María J. Yebra, Vicente Monedero, and Jesús Rodríguez-Díaz. 2021. "Microbiota Depletion Promotes Human Rotavirus Replication in an Adult Mouse Model" Biomedicines 9, no. 7: 846. https://doi.org/10.3390/biomedicines9070846

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