Tumor-Infiltrating Lymphocytes in the Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma: Systematic Review and Meta-Analysis †
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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References | Analytic Cohort | Covariant | Sample | Stage | Treatment | Technique | Other Biomarkers |
---|---|---|---|---|---|---|---|
Vassilakopoulou et al., 2016 [33] | 260 | High stromal TIL (≥30%) | Postoperative sample | All | S, S+RT | HE in WS | PD-L1 (IHC and mRNA) |
Wang et al., 2016 [34] | 120 | High stromal TIL (≥30%) | Postoperative sample | All | S+RT, S+CRT | HE in WS | No |
Hoesli et al., 2018 [35] | 183 | High tumor-infiltrating CD8+ (dichotomized at the median) | Postoperative sample | All | S (post RT, CRT) | IHC in TMAs | PD-L1 CPS (IHC) |
Mann et al., 2019 [36] | 183 | High tumor-infiltrating CD103+ and CD4+ | Postoperative sample | All | S (post RT, CRT) | IHC in TMAs | No |
Spector et al., 2019 [9] | 74 | High tumor-infiltrating CD8+ (dichotomized at the median) and high tumoral TILws (0.35 × CD8+ 0.35 × CD4+ 0.3 × FOXP3+) | Incisional biopsies | All | S, S+(C)RT, RT, CRT | IHC in TMAs | No |
Zhou et al., 2019 [37] | 71 | High tumor- and stromal-infiltrating CD8+ and high stromal-infiltrating CD3+ (dichotomized at the median) | Postoperative sample | All | S | IHC in WS | CD163 (IHC) |
Chatzopoulos et al., 2020 [38] | 283 | Stromal TIL (10% increments) and high tumor-infiltrating CD8+ (dichotomized at the median) | Postoperative sample | All | S, S+RT | HE in WS and IHC in TMAs | No |
Sánchez-Canteli et al., 2020 [39] | 67 | High tumor-infiltrating CD8+ (dichotomized at the median) | Postoperative sample | All | S, S+RT | IHC in TMAs | PD-L1 CPS and TPS (IHC) |
Tagliabue et al., 2020 [40] | 56 | High stromal TIL (≥5%) | Postoperative sample | III/IV | S, S+(C)RT, RT, CRT | HE in WS | No |
Zhang et al., 2020 [41] | 41 | Immunoscore (2–4) vs (0–1) (CD3+/CD8+) | Postoperative sample | All | S | IHC in WS | CD66b+ (IHC) |
Franz et al., 2021 [42] | 60 | High stromal TIL (≥30%) | Postoperative sample | All | S, S+RT | HE in WS | PD-L1 CPS (IHC) NLR |
References | Key Findings |
---|---|
Vassilakopoulou et al., 2016 [33] | Increased TIL density in tumor stroma was associated with better outcome in laryngeal squamous cell carcinoma. |
Wang et al., 2016 [34] | High TIL density in tumor stroma, which represents the local inflammation, was predictive of longer OS and RFS. |
Hoesli et al., 2018 [35] | CD8+ TIL status was associated with a significant improvement in DFS and DSS in patients with recurrent/persistent laryngeal squamous cell carcinoma surgically treated after RT/CRT. |
Mann et al., 2019 [36] | An immune profile driven by CD103+ TIL content, alone and in combination with CD4+ TIL content, was a prognostic biomarker of survival in patients with recurrent/persistent LSCC. |
Spector et al., 2019 [9] | The levels of TIL were an independent prognostic factor in patients with head and neck squamous cell carcinoma, including laryngeal squamous cell carcinoma, treated with different modalities. Subsets of TILs and combined TILs scores may be clinically useful predictive and prognostic factors. |
Zhou et al., 2019 [37] | High density of peritumoral CD3+ and CD8+ immune cells in both tumor stroma and tumor nests was significantly associated with a favorable OS. |
Chatzopoulos et al., 2020 [38] | Favorable prognostic impact of higher TIL density in laryngeal squamous cell carcinoma patients. The assessment of CD8+ infiltrates does not seem to offer additional prognostic information over the morphologically assessed TIL density. |
Sánchez-Canteli et al., 2020 [39] | High tumoral infiltration by CD8+ TIL was associated with better survival outcomes. |
Tagliabue et al., 2020 [40] | Low TIL and altered expression of specific genes associated with tumor–immune systems interactions emerged as independent risk factors, associated with poor prognosis and relapse within 2 years in advanced laryngeal squamous cell carcinoma. |
Zhang et al., 2020 [41] | High density of CD3+ TIL were associated with better OS. Patients with an Immunoscore of 0–1 experienced the worst OS and DFS, compared with Immunoscore 2–4. |
Franz et al., 2021 [42] | TIL count rate ≥30% was associated with higher DFS and reduced recurrence risk. |
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Rodrigo, J.P.; Sánchez-Canteli, M.; López, F.; Wolf, G.T.; Hernández-Prera, J.C.; Williams, M.D.; Willems, S.M.; Franchi, A.; Coca-Pelaz, A.; Ferlito, A. Tumor-Infiltrating Lymphocytes in the Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma: Systematic Review and Meta-Analysis. Biomedicines 2021, 9, 486. https://doi.org/10.3390/biomedicines9050486
Rodrigo JP, Sánchez-Canteli M, López F, Wolf GT, Hernández-Prera JC, Williams MD, Willems SM, Franchi A, Coca-Pelaz A, Ferlito A. Tumor-Infiltrating Lymphocytes in the Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma: Systematic Review and Meta-Analysis. Biomedicines. 2021; 9(5):486. https://doi.org/10.3390/biomedicines9050486
Chicago/Turabian StyleRodrigo, Juan P., Mario Sánchez-Canteli, Fernando López, Gregory T. Wolf, Juan C. Hernández-Prera, Michelle D. Williams, Stefan M. Willems, Alessandro Franchi, Andrés Coca-Pelaz, and Alfio Ferlito. 2021. "Tumor-Infiltrating Lymphocytes in the Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma: Systematic Review and Meta-Analysis" Biomedicines 9, no. 5: 486. https://doi.org/10.3390/biomedicines9050486