Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Ethics Issues
2.2. Cell Isolation and Culture
2.2.1. DPSCs
2.2.2. BFPSCs
2.3. Success Rate of Isolation of DPSCs and BFPSCs
2.4. Cell Number and Morphology Evaluation
2.5. Proliferation Assay
2.6. Osteodifferentiation Assays
2.7. Flow Cytometry Analysis of DPSCs and BFPSCs Phenotypes
2.8. Osteocalcin Evaluation
2.9. RNA Extraction and Real-Time PCR Analysis
2.10. Statistical Analysis
3. Results
3.1. Success Rates in Isolating MSCs from BFP and DP
3.2. BFPSCs Show a MSC Immunophenotype
3.3. BFPSCs and DPSCs Differentiate into Osteoblast-Like
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Patient | Gender | Age | Tooth Site | Buccal Fat Pad (BFP) Site |
---|---|---|---|---|
1 | M | 20 | 18 | R |
2 | F | 26 | 28 | L |
3 | F | 23 | 28 | L |
4 | F | 27 | 28 | L |
5 | M | 22 | 18 | R |
6 | M | 30 | 18 | R |
7 | F | 21 | 28 | L |
8 | M | 27 | 18 | R |
9 | M | 26 | 28 | L |
10 | M | 24 | 18 | R |
11 | F | 25 | 18 | R |
12 | M | 22 | 28 | L |
CD44 | CD73 | CD90 | CD105 | |
---|---|---|---|---|
BFPSCs | 95.4 ± 2.1 | 84.5 ± 7.8 | 98.9 ± 0.8 | 76.8 ± 23 |
DPSCs | 97.9 ± 1.2 | 95.1 ± 3.4 | 98.9 ± 0.7 | 65.3 ± 30.1 |
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Genova, T.; Cavagnetto, D.; Tasinato, F.; Petrillo, S.; Ruffinatti, F.A.; Mela, L.; Carossa, M.; Munaron, L.; Roato, I.; Mussano, F. Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor. Biomedicines 2021, 9, 265. https://doi.org/10.3390/biomedicines9030265
Genova T, Cavagnetto D, Tasinato F, Petrillo S, Ruffinatti FA, Mela L, Carossa M, Munaron L, Roato I, Mussano F. Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor. Biomedicines. 2021; 9(3):265. https://doi.org/10.3390/biomedicines9030265
Chicago/Turabian StyleGenova, Tullio, Davide Cavagnetto, Fabio Tasinato, Sara Petrillo, Federico Alessandro Ruffinatti, Luca Mela, Massimo Carossa, Luca Munaron, Ilaria Roato, and Federico Mussano. 2021. "Isolation and Characterization of Buccal Fat Pad and Dental Pulp MSCs from the Same Donor" Biomedicines 9, no. 3: 265. https://doi.org/10.3390/biomedicines9030265