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Article

Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin Hydrogels

1
Department of Materials Science and Engineering, Institute Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, Germany
2
IUF-Leibniz Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 Duesseldorf, Germany
3
Electron Microscopy Facility, Institute of Pathology, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany
4
Medical Faculty, Heinrich-Heine-University, 40225 Düsseldorf, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Elvira De Giglio and Maria A. Bonifacio
Biomedicines 2021, 9(3), 261; https://doi.org/10.3390/biomedicines9030261
Received: 9 February 2021 / Revised: 27 February 2021 / Accepted: 2 March 2021 / Published: 5 March 2021
(This article belongs to the Special Issue Hydrogels for Biomedical Application)
Biodegradable hydrogels that promote stem cell differentiation into neurons in three dimensions (3D) are highly desired in biomedical research to study drug neurotoxicity or to yield cell-containing biomaterials for neuronal tissue repair. Here, we demonstrate that oxidized alginate-gelatin-laminin (ADA-GEL-LAM) hydrogels facilitate neuronal differentiation and growth of embedded human induced pluripotent stem cell (hiPSC) derived neurospheres. ADA-GEL and ADA-GEL-LAM hydrogels exhibiting a stiffness close to ~5 kPa at initial cell culture conditions of 37 °C were prepared. Laminin supplemented ADA-GEL promoted an increase in neuronal differentiation in comparison to pristine ADA-GEL, with enhanced neuron migration from the neurospheres to the bulk 3D hydrogel matrix. The presence of laminin in ADA-GEL led to a more than two-fold increase in the number of neurospheres with migrated neurons. Our findings suggest that laminin addition to oxidized alginate—gelatin hydrogel matrices plays a crucial role to tailor oxidized alginate-gelatin hydrogels suitable for 3D neuronal cell culture applications. View Full-Text
Keywords: oxidized alginate; laminin; hydrogels; human induced pluripotent stem cells (hiPSC); neurospheres; tissue engineering; bioprinting oxidized alginate; laminin; hydrogels; human induced pluripotent stem cells (hiPSC); neurospheres; tissue engineering; bioprinting
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MDPI and ACS Style

Distler, T.; Lauria, I.; Detsch, R.; Sauter, C.M.; Bendt, F.; Kapr, J.; Rütten, S.; Boccaccini, A.R.; Fritsche, E. Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin Hydrogels. Biomedicines 2021, 9, 261. https://doi.org/10.3390/biomedicines9030261

AMA Style

Distler T, Lauria I, Detsch R, Sauter CM, Bendt F, Kapr J, Rütten S, Boccaccini AR, Fritsche E. Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin Hydrogels. Biomedicines. 2021; 9(3):261. https://doi.org/10.3390/biomedicines9030261

Chicago/Turabian Style

Distler, Thomas, Ines Lauria, Rainer Detsch, Clemens M. Sauter, Farina Bendt, Julia Kapr, Stephan Rütten, Aldo R. Boccaccini, and Ellen Fritsche. 2021. "Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin Hydrogels" Biomedicines 9, no. 3: 261. https://doi.org/10.3390/biomedicines9030261

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