Oral Squamous Cell Carcinoma Is Associated with a Low Thrombosis Risk Due to Storage Pool Deficiency in Platelets
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies and Reagent
2.2. Cell Culture
2.3. Mice
2.4. TF and TFPI Activity Assay
2.5. Mice Platelet Rich Plasma Preparation (PRP)
2.6. Induction of Platelet Aggregation by OSCC Cell
2.7. Platelet Aggregometry
2.8. Mouse Model of Tongue Squamous Cell Carcinoma
2.9. Intravital Microscopy and Laser-Induced Injury
2.10. Optical Microscopy
2.11. Fluorescent Microscopy
2.12. Transmission Electron Microscopy (TEM)
2.13. Statistics
3. Results
3.1. OSCC Cells Induce Platelet Aggregation In Vitro
3.2. Platelet Aggregates and Fibrin Deposits Are Locally Detected at the Site of the Primary Squamous Cell Carcinoma Tumors
3.3. Tongue SCC Is Associated with a Reduction in Systemic Thrombosis Risk in Living Mice
3.4. Tongue Tumors Are Associated with a Decrease in Platelet Reactivity
3.5. Tongue Tumors Are Associated with Granule Pool Deficiency
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tumor-Free Mice (WT) Mean (SD) | Tongue Tumor Mice (AT84) Mean (SD) | P (Wilcoxon) | |
---|---|---|---|
Platelets (nb × 103/μL) | 883.2 (399.6) | 846.5 (254) | 0.893 (NS) |
Neutrophils (nb/μL) | 1238 (486) | 1381 (479) | 0.588 (NS) |
Monocytes (nb/μL) | 159.8 (104.8) | 242.7 (185.5) | 0.168 (NS) |
Lymphocytes (nb/μL) | 3095 (1851) | 2682 (1571) | 0.685 (NS) |
Tail bleeding time (seconds) | 128.9 (28.48) | 146.2 (48.62) | 0.361 (NS) |
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Haen, P.; Crescence, L.; Mege, D.; Altié, A.; Dubois, C.; Panicot-Dubois, L. Oral Squamous Cell Carcinoma Is Associated with a Low Thrombosis Risk Due to Storage Pool Deficiency in Platelets. Biomedicines 2021, 9, 228. https://doi.org/10.3390/biomedicines9030228
Haen P, Crescence L, Mege D, Altié A, Dubois C, Panicot-Dubois L. Oral Squamous Cell Carcinoma Is Associated with a Low Thrombosis Risk Due to Storage Pool Deficiency in Platelets. Biomedicines. 2021; 9(3):228. https://doi.org/10.3390/biomedicines9030228
Chicago/Turabian StyleHaen, Pierre, Lydie Crescence, Diane Mege, Alexandre Altié, Christophe Dubois, and Laurence Panicot-Dubois. 2021. "Oral Squamous Cell Carcinoma Is Associated with a Low Thrombosis Risk Due to Storage Pool Deficiency in Platelets" Biomedicines 9, no. 3: 228. https://doi.org/10.3390/biomedicines9030228