miR-154 Influences HNSCC Development and Progression through Regulation of the Epithelial-to-Mesenchymal Transition Process and Could Be Used as a Potential Biomarker
Abstract
:1. Introduction
2. Materials and Methods
2.1. Data Collection
2.2. Pathological and Clinical Analysis
2.3. Functional Enrichment Analysis and Prediction of Genes’ Function
2.4. Target Analysis
2.5. Immune Profile Analysis
2.6. Statistical Analysis
2.7. Availability of Data and Materials
3. Results
3.1. miR-154-5p and miR-154-3p Levels Differ Depending on the Clinicopathological Parameters
3.2. Patients with Low Expression of miR-154-3p Had a Significantly Extended Overall Survival
3.3. Higher Expression of miR-154-5p and miR-154-3p Was Associated with the Upregulation of Oncogenic Pathways in the HNSCC Patients
3.4. Patients with Low Levels of Both miR-154 Mature Strands Have a More Favorable Immunological Profile
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Group | miR-154-5p | miR-154-3p | ||||
---|---|---|---|---|---|---|---|
Cases | Mean ± SEM | p | Cases | Mean ± SEM | p | ||
Age | ≤ 60 | n = 250 | 1.919 ± 0.8643 | 0.2086 | n = 247 | 1.491 ± 0.7910 | 0.2127 |
> 61 | n = 269 | 2.013 ± 0.8272 | n = 264 | 1.582 ± 0.8511 | |||
Gender | Female | n = 147 | 1.950 ± 0.7729 | 0.7350 | n = 43 | 1.490 ± 0.7226 | 0.3860 |
Male | n = 373 | 1.978 ± 0.8747 | n = 369 | 1.560 ± 0.8606 | |||
Alcohol | Yes | n = 337 | 1.959 ± 0.8285 | 0.9619 | n = 331 | 1.539 ± 0.8437 | 0.8766 |
No | n = 173 | 1.962 ± 0.8560 | n = 171 | 1.527 ± 0.7874 | |||
Smoking | No/ex | n = 203 | 1.925 ± 0.8307 | 0.2880 | n = 201 | 1.529 ± 0.8028 | 0.8000 |
Yes | n = 302 | 2.006 ± 0.8551 | n = 296 | 1.549 ± 0.8442 | |||
Cancer stage | I + II | n = 125 | 2.145 ± 0.8002 | 0.0077 | n = 123 | 1.625 ± 0.7244 | 0.1674 |
III + IV | n = 382 | 1.913 ± 0.8548 | n = 376 | 1.510 ± 0.8291 | |||
T-stage | T1 + T2 | n = 189 | 2.001 ± 0.8369 | 0.5924 | n = 189 | 1.511 ± 0.7406 | 0.5106 |
T3 + T4 | n = 316 | 1.959 ± 0.8497 | n = 308 | 1.560 ± 0.8428 | |||
N-stage | N0 | n = 252 | 2.071 ± 0.8426 | 0.0066 | n = 245 | 1.623 ± 0.8086 | 0.0208 |
N1 + N2 + N3 | n = 248 | 1.866 ± 0.8409 | n = 246 | 1.455 ± 0.7938 | |||
Grade | G1 + G2 | n = 370 | 1.913 ± 0.8247 | 0.0017 | n = 361 | 1.507 ± 0.7755 | 0.0589 |
G3 + G4 | n = 126 | 2.182 ± 0.8299 | n = 127 | 1.667 ± 0.9413 | |||
Perineural invasion | Positive | n = 171 | 2.090 ± 0.7819 | 0.0777 | n = 169 | 1.682 ± 0.8359 | 0.1173 |
Negative | n = 192 | 1.936 ± 0.8610 | n = 188 | 1.538 ± 0.8889 | |||
Lymph node neck dissection | Positive | n = 416 | 1.986 ± 0.8395 | 0.4054 | n = 407 | 1.564 ± 0.8272 | 0.2810 |
Negative | n = 102 | 1.908 ± 0.8814 | n = 102 | 1.466 ± 0.8081 | |||
HPV p16 status | Positive | n = 38 | 1.492 ± 0.9784 | 0.0102 | n = 36 | 1.208 ± 0.7566 | 0.0277 |
Negative | n = 65 | 1.943 ± 0.7552 | n = 65 | 1.534 ± 0.6708 |
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Tomaszewska, W.; Kozłowska-Masłoń, J.; Baranowski, D.; Perkowska, A.; Szałkowska, S.; Kazimierczak, U.; Severino, P.; Lamperska, K.; Kolenda, T. miR-154 Influences HNSCC Development and Progression through Regulation of the Epithelial-to-Mesenchymal Transition Process and Could Be Used as a Potential Biomarker. Biomedicines 2021, 9, 1894. https://doi.org/10.3390/biomedicines9121894
Tomaszewska W, Kozłowska-Masłoń J, Baranowski D, Perkowska A, Szałkowska S, Kazimierczak U, Severino P, Lamperska K, Kolenda T. miR-154 Influences HNSCC Development and Progression through Regulation of the Epithelial-to-Mesenchymal Transition Process and Could Be Used as a Potential Biomarker. Biomedicines. 2021; 9(12):1894. https://doi.org/10.3390/biomedicines9121894
Chicago/Turabian StyleTomaszewska, Weronika, Joanna Kozłowska-Masłoń, Dawid Baranowski, Anna Perkowska, Sandra Szałkowska, Urszula Kazimierczak, Patricia Severino, Katarzyna Lamperska, and Tomasz Kolenda. 2021. "miR-154 Influences HNSCC Development and Progression through Regulation of the Epithelial-to-Mesenchymal Transition Process and Could Be Used as a Potential Biomarker" Biomedicines 9, no. 12: 1894. https://doi.org/10.3390/biomedicines9121894