Relationship between Vitamin D Status and Antibody Response to COVID-19 mRNA Vaccination in Healthy Adults
, Kamil Demircan 1,2, Raban Arved Heller 1,3,4, Ines Maria Hirschbil-Bremer 5, Joachim Diegmann 5, Manuel Bachmann 5, Arash Moghaddam 6 and Lutz Schomburg 1,*
Round 1
Reviewer 1 Report
This is an interesting study reporting very important observations. In fact, the authors show that in healthy adults with moderate vitamin D status, the immune response to vaccination with SARS-CoV-2 vaccines is independent on the 25‑hydroxy-cholecalciferol [25(OH)D] (vitamin D) content. These are clearly noticeable observations, since vitamin D is considered as one of the micronutrients capable to influence the immune system. These observations are counterintuitive, as vitamin D is know to show multiple beneficial properties including the importance of adequate vitamin D status for the reduction of the risk of the SARS-CoV-2 infection and severe COVID-19 course. The manuscript is well-written and concise. Since it is addressing an important issue, it definitely will have a noticeable impact.
Author Response
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Author Response File: 
Author Response.pdf
Reviewer 2 Report
In this paper, the authors have analysed the possible relationship between Vitamin D serum levels and the titer of antibodies against SARS-CoV-2 after vaccination of healthy subjects. Unfortunately results are negative.
Major comments:
1) As clearly expressed in the discussion part, hitherto, for other infectious diseases (flu, streptococcus pneumonia or neisseiria meningitidis...) no relationships were found between antibody responses to vaccination and vitamin D levels. Therefore, the authors should better explained, in the intoduction section, why they hypothesized that it could be different for SARS-CoV-2. In addition, they indicate in the discussion that vitamin D levels is not related to the severity of COVID-19...
2) All the data concerning the accuracy of the measurement of Vitamin D are not related to the topic of the paper and should be deleted. Similarly, much data are available in the kinetic of antibodies after SARS-CoV-2 vaccination in health care workers (HCW) and the data presented should be summarized,. Fig 1 is not necessary.
Minor comment:
It could be interesting to indicate, in table 1, the percentage of HCW who are positive for IgG antibodies as well as the percentage of HCW deficient in Vit D.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
