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Article

ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads

by 1 and 1,2,*
1
School of Mechanical engineering, Korea University, Seoul 02841, Korea
2
Engineering Research Center for Biofluid Biopsy, Korea University, Seoul 02841, Korea
*
Author to whom correspondence should be addressed.
Biomedicines 2021, 9(1), 28; https://doi.org/10.3390/biomedicines9010028
Received: 1 December 2020 / Revised: 24 December 2020 / Accepted: 28 December 2020 / Published: 2 January 2021
(This article belongs to the Special Issue Liquid Biopsies in Cancer Diagnosis, Monitoring and Prognosis)
Extracellular vesicles (EVs) are considered essential biomarkers in liquid biopsies. Despite intensive efforts aimed at employing EVs in a clinical setting, workable approaches are currently limited owing to the fact that EV-isolation technologies are still in a nascent stage. This study introduces a magnetic bead-based ion exchange platform for isolating EVs called ExoCAS-2 (exosome clustering and scattering). Owing to their negative charge, exosomes can easily adhere to magnetic beads coated with a polycationic polymer. Owing to the features of magnetic beads, exosomes can be easily processed via washing and elution steps and isolated with high purity and yield within 40 min. The present results confirmed the isolation of exosomes through analyses of size distribution, morphology, surface and internal protein markers, and exosomal RNA. Compared with the commercially available methods, the proposed method showed superior performance in terms of key aspects, including operation time, purity, and recovery rate. This highlights the potential of this magnetic bead-based ion exchange platform for isolating exosomes present in blood plasma. View Full-Text
Keywords: exosome; isolation; cationic polymer; magnetic beads; ion exchange exosome; isolation; cationic polymer; magnetic beads; ion exchange
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MDPI and ACS Style

Kim, H.; Shin, S. ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads. Biomedicines 2021, 9, 28. https://doi.org/10.3390/biomedicines9010028

AMA Style

Kim H, Shin S. ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads. Biomedicines. 2021; 9(1):28. https://doi.org/10.3390/biomedicines9010028

Chicago/Turabian Style

Kim, Hyunsung, and Sehyun Shin. 2021. "ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads" Biomedicines 9, no. 1: 28. https://doi.org/10.3390/biomedicines9010028

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