Phospholipid Arachidonic Acid Remodeling During Phagocytosis in Mouse Peritoneal Macrophages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. Measurement of Phospholipid Arachidonate Remodeling
2.4. Gas Chromatography/Mass Spectrometry (GC/MS) Analyses
2.5. Liquid Chromatography/Mass Spectrometry (LC/MS) Analyses of Phospholipids
2.6. Liquid Chromatography/Mass Spectrometry (LC/MS) Analyses of Eicosanoids
2.7. Data Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AA | Arachidonic acid |
CoA-IT | Coenzyme A-independent transacylase |
GC/MS | Gas chromatography coupled to mass spectrometry |
LC/MS | Liquid chromatography coupled to mass spectrometry |
OpZ | Opsonized zymosan |
PC | Choline-containing glycerophospholipid |
PE | Ethanolamine-containing glycerophospholipid |
PI | Phosphatidylinositol |
PLA2 | Phospholipase A2 |
cPLA2α | Cytosolic group IVA phospholipase A2 |
iPLA2 | Calcium-independent phospholipase A2 |
sPLA2 | Secreted phospholipase A2 |
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Gil-de-Gómez, L.; Monge, P.; Rodríguez, J.P.; Astudillo, A.M.; Balboa, M.A.; Balsinde, J. Phospholipid Arachidonic Acid Remodeling During Phagocytosis in Mouse Peritoneal Macrophages. Biomedicines 2020, 8, 274. https://doi.org/10.3390/biomedicines8080274
Gil-de-Gómez L, Monge P, Rodríguez JP, Astudillo AM, Balboa MA, Balsinde J. Phospholipid Arachidonic Acid Remodeling During Phagocytosis in Mouse Peritoneal Macrophages. Biomedicines. 2020; 8(8):274. https://doi.org/10.3390/biomedicines8080274
Chicago/Turabian StyleGil-de-Gómez, Luis, Patricia Monge, Juan P. Rodríguez, Alma M. Astudillo, María A. Balboa, and Jesús Balsinde. 2020. "Phospholipid Arachidonic Acid Remodeling During Phagocytosis in Mouse Peritoneal Macrophages" Biomedicines 8, no. 8: 274. https://doi.org/10.3390/biomedicines8080274