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Open AccessArticle

Hyperthermia Disturbs and Delays Spontaneous Differentiation of Human Embryoid Bodies

1
Soonchunhyang Institute of Medi-bio Science (SIMS), Soon Chun Hyang University, Cheonan 31151, Korea
2
Department of Medicine, Konkuk University School of Medicine and Mirae Cell Bio Co. LTD., Seoul 05029, Korea
3
Department of Neurosurgery, College of Medicine, Soonchunhyang University, Cheonan Hospital, Cheonan 31151, Korea
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Biomedicines 2020, 8(6), 176; https://doi.org/10.3390/biomedicines8060176
Received: 11 May 2020 / Revised: 16 June 2020 / Accepted: 19 June 2020 / Published: 26 June 2020
(This article belongs to the Section Model Systems in Research and Development)
Various types of stress stimuli have been shown to threaten the normal development of embryos during embryogenesis. Prolonged heat exposure is the most common stressor that poses a threat to embryo development. Despite the extensive investigation of heat stress control mechanisms in the cytosol, the endoplasmic reticulum (ER) heat stress response remains unclear. In this study, we used human embryonic stem cells (hESCs) to examine the effect of heat stress on early embryonic development, specifically alterations in the ER stress response. In a hyperthermic (42 °C) culture, ER stress response genes involved in hESC differentiation were induced within 1 h of exposure, which resulted in disturbed and delayed differentiation. In addition, hyperthermia increased the expression levels of activating transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP) genes, which are associated with the protein kinase RNA-like endoplasmic reticulum kinase (PERK) signaling pathway. Furthermore, we demonstrated that tauroursodeoxycholic acid, a chemical chaperone, mitigated the delayed differentiation under hyperthermia. Our study identified novel gene markers in response to hyperthermia-induced ER stress on hESCs, thereby providing further insight into the mechanisms that regulate human embryogenesis. View Full-Text
Keywords: hyperthermia; embryogenesis; human embryonic stem cells; endoplasmic reticulum; stress response hyperthermia; embryogenesis; human embryonic stem cells; endoplasmic reticulum; stress response
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MDPI and ACS Style

Kwon, J.H.; Kim, H.K.; Ha, T.W.; Im, J.S.; Song, B.H.; Hong, K.S.; Oh, J.S.; Han, J.; Lee, M.R. Hyperthermia Disturbs and Delays Spontaneous Differentiation of Human Embryoid Bodies. Biomedicines 2020, 8, 176.

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