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Open AccessArticle

Leucine Potentiates Glucose-mediated 18F-FDG Uptake in Brown Adipose Tissue via β-Adrenergic Activation

1
Biology, Lakehead University, Thunder Bay, ON P7B 5E1, Canada
2
Medical Sciences, Lakehead University Faculty of Medicine, Thunder Bay, ON P7B 5E1, Canada
3
Northern Ontario School of Medicine, Medical Sciences Division, Thunder Bay, ON P7B 5E1, Canada
4
Chemistry, University of Saskatchewan, Saskatoon, SK S7N 5C9, Canada
5
Thunder Bay Regional Health Research Institute, Thunder Bay, ON P7A 7T1, Canada
*
Authors to whom correspondence should be addressed.
Biomedicines 2020, 8(6), 159; https://doi.org/10.3390/biomedicines8060159
Received: 28 April 2020 / Revised: 27 May 2020 / Accepted: 10 June 2020 / Published: 13 June 2020
(This article belongs to the Section Tumor Cell Biology)
Significant depots of brown adipose tissue (BAT) have been identified in many adult humans through positron emission tomography (PET), with the amount of BAT being inversely correlated with obesity. As dietary activation of BAT has implications for whole body glucose metabolism, leucine was used in the present study to determine its ability to promote BAT activation resulting in increased glucose uptake. In order to assess this, 2-deoxy-2-(fluorine-18)fluoro-d-glucose (18F-FDG) uptake was measured in C57BL/6 mice using microPET after treatment with leucine, glucose, or both in interscapular BAT (IBAT). Pretreatment with propranolol (PRP) was used to determine the role of β-adrenergic activation in glucose and leucine-mediated 18F-FDG uptake. Analysis of maximum standardized uptake values (SUVMAX) determined that glucose administration increased 18F-FDG uptake in IBAT by 25.3%. While leucine did not promote 18F-FDG uptake alone, it did potentiate glucose-mediated 18F-FDG uptake, increasing 18F-FDG uptake in IBAT by 22.5%, compared to glucose alone. Pretreatment with PRP prevented the increase in IBAT 18F-FDG uptake following the combination of glucose and leucine administration. These data suggest that leucine is effective in promoting BAT 18F-FDG uptake through β-adrenergic activation in combination with glucose. View Full-Text
Keywords: positron emission tomography; non-shivering thermogenesis; diet-induced thermogenesis; glucose metabolism; obesity; propranolol; interscapular brown adipose tissue; UCP-1 positron emission tomography; non-shivering thermogenesis; diet-induced thermogenesis; glucose metabolism; obesity; propranolol; interscapular brown adipose tissue; UCP-1
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Huska, B.; Niccoli, S.; Phenix, C.P.; Lees, S.J. Leucine Potentiates Glucose-mediated 18F-FDG Uptake in Brown Adipose Tissue via β-Adrenergic Activation. Biomedicines 2020, 8, 159.

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