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Open AccessArticle

Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage

1
MOE Key Laboratory for Regenerative Medicine, School of Biomedical Sciences, Chinese University of Hong Kong, Shatin, Hong Kong
2
Chinese University of Hong Kong-University of Southampton Joint Laboratory for Stem Cell and Regenerative Medicine, School of Biomedical Sciences, Chinese University of Hong Kong, Shatin, Hong Kong
*
Author to whom correspondence should be addressed.
Biomedicines 2020, 8(10), 397; https://doi.org/10.3390/biomedicines8100397
Received: 7 September 2020 / Revised: 28 September 2020 / Accepted: 3 October 2020 / Published: 10 October 2020
(This article belongs to the Special Issue Stem Cell Signaling)
BRISC and BRCA1-A complex member 2 (Babam2) plays an essential role in promoting cell cycle progression and preventing cellular senescence. Babam2-deficient fibroblasts show proliferation defect and premature senescence compared with their wild-type (WT) counterpart. Pluripotent mouse embryonic stem cells (mESCs) are known to have unlimited cell proliferation and self-renewal capability without entering cellular senescence. Therefore, studying the role of Babam2 in ESCs would enable us to understand the mechanism of Babam2 in cellular aging, cell cycle regulation, and pluripotency in ESCs. For this study, we generated Babam2 knockout (Babam2−/−) mESCs to investigate the function of Babam2 in mESCs. We demonstrated that the loss of Babam2 in mESCs leads to abnormal G1 phase retention in response to DNA damage induced by gamma irradiation or doxorubicin treatments. Key cell cycle regulators, CDC25A and CDK2, were found to be degraded in Babam2−/− mESCs following gamma irradiation. In addition, Babam2−/− mESCs expressed p53 strongly and significantly longer than in control mESCs, where p53 inhibited Nanog expression and G1/S cell cycle progression. The combined effects significantly reduced developmental pluripotency in Babam2−/− mESCs. In summary, Babam2 maintains cell cycle regulation and pluripotency in mESCs in response to induced DNA damage. View Full-Text
Keywords: Babam2; embryonic stem cells; cell cycle; DNA damage; pluripotency; senescence Babam2; embryonic stem cells; cell cycle; DNA damage; pluripotency; senescence
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MDPI and ACS Style

Chung, C.Y.T.; Lo, P.H.Y.; Lee, K.K.H. Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage. Biomedicines 2020, 8, 397.

AMA Style

Chung CYT, Lo PHY, Lee KKH. Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage. Biomedicines. 2020; 8(10):397.

Chicago/Turabian Style

Chung, Cheuk Y.T.; Lo, Paulisally H.Y.; Lee, Kenneth K.H. 2020. "Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage" Biomedicines 8, no. 10: 397.

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