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Article

Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)

by 1,2,†, 1,†, 1, 1,2 and 1,3,*
1
Interdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, Denmark
2
Omiics ApS, Åbogade 15, 8200 Aarhus N, Denmark
3
Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark
*
Author to whom correspondence should be addressed.
These authors contributed equally to the paper.
Biomedicines 2020, 8(10), 387; https://doi.org/10.3390/biomedicines8100387
Received: 14 September 2020 / Accepted: 24 September 2020 / Published: 28 September 2020
Multipotent stem cells (MSCs) are used in various therapeutic applications based on their paracrine secretion activity. Here, we set out to identify and characterize the paracrine factors released during osteoblastogenesis, with a special focus on small non-coding RNAs released in extracellular vesicles (EVs). Bone marrow stem cells (BMSCs) and adipose stem cells (ASCs) from healthy human donors were used as representatives of MSCs. We isolated EVs secreted before and after induction of osteoblastic differentiation and found that the EVs contained a specific subset of microRNAs (miRNAs) and tRNA-derived small RNAs (tsRNA) compared to their parental cells. Osteoblastic differentiation had a larger effect on the small RNA profile of BMSC-EVs relative to ASC-EVs. Our data showed that EVs from different MSC origin exhibited distinct expression profiles of small RNA profiles when undergoing osteoblastogenesis, a factor that should be taken into consideration for stem cell therapy. View Full-Text
Keywords: multipotent stem cell; bone marrow stem cell; adipose stem cells; osteoblastogenesis; extracellular vesicles; microRNA; tRNA-derived small RNA multipotent stem cell; bone marrow stem cell; adipose stem cells; osteoblastogenesis; extracellular vesicles; microRNA; tRNA-derived small RNA
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MDPI and ACS Style

Yan, Y.; Chang, C.; Su, J.; Venø, M.T.; Kjems, J. Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs). Biomedicines 2020, 8, 387. https://doi.org/10.3390/biomedicines8100387

AMA Style

Yan Y, Chang C, Su J, Venø MT, Kjems J. Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs). Biomedicines. 2020; 8(10):387. https://doi.org/10.3390/biomedicines8100387

Chicago/Turabian Style

Yan, Yan; Chang, Clare; Su, Junyi; Venø, Morten T.; Kjems, Jørgen. 2020. "Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)" Biomedicines 8, no. 10: 387. https://doi.org/10.3390/biomedicines8100387

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