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Evaluation of Angiogenesis Assays

1
Department of Health, Division of Environmental Health Sciences, Albany, NY 12237, USA
2
The Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, NY 12144, USA
3
Department of Medicine, Albany Medical College, Albany, NY 12208, USA
*
Author to whom correspondence should be addressed.
Biomedicines 2019, 7(2), 37; https://doi.org/10.3390/biomedicines7020037
Received: 1 March 2019 / Revised: 8 May 2019 / Accepted: 10 May 2019 / Published: 16 May 2019
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PDF [217 KB, uploaded 16 May 2019]

Abstract

Angiogenesis assays allow for the evaluation of pro- or anti-angiogenic activity of endogenous or exogenous factors (stimulus or inhibitors) through investigation of their pro-or anti- proliferative, migratory, and tube formation effects on endothelial cells. To model the process of angiogenesis and the effects of biomolecules on that process, both in vitro and in vivo methods are currently used. In general, in vitro methods monitor specific stages in the angiogenesis process and are used for early evaluations, while in vivo methods more accurately simulate the living microenvironment to provide more pertinent information. We review here the current state of angiogenesis assays as well as their mechanisms, advantages, and limitations. View Full-Text
Keywords: angiogenesis; pro-angiogenesis; anti-angiogenesis; anticancer; growth factors; integrin αvβ3; vascular endothelial growth factor (VEGF); vascularization; endothelial cells angiogenesis; pro-angiogenesis; anti-angiogenesis; anticancer; growth factors; integrin αvβ3; vascular endothelial growth factor (VEGF); vascularization; endothelial cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Stryker, Z.I.; Rajabi, M.; Davis, P.J.; Mousa, S.A. Evaluation of Angiogenesis Assays. Biomedicines 2019, 7, 37.

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