Short-Term Periodic Fasting Reduces Ischemia-Induced Necrosis in Musculocutaneous Flap Tissue
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. PF Regimen
2.3. Anesthesia
2.4. Dorsal Skinfold Chamber Flap Model
2.5. Intravital Fluorescence Microscopy
2.6. Histology and Immunohistochemistry
2.7. Statistical Analysis
3. Results
3.1. Intravital Fluorescence Microscopy
3.2. Histological and Immunohistochemical Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Volumetric Blood Flow [pL/s] | d1 | d3 | d5 | d7 | d10 | |
---|---|---|---|---|---|---|
Arterioles | ||||||
prox. control | 348 ± 53 | 404 ± 44 | 554 ± 91 | 767 ± 130 | 1019 ± 150 | |
PF | 643 ± 126 * | 1165 ± 178 * | 1438 ± 282 * | 1637 ± 209 * | 1812 ± 178 * | |
med. control | 325 ± 98 | 389 ± 104 | 530 ± 124 | 770 ± 203 | 1087 ± 247 | |
PF | 742 ± 149 * | 1367 ± 230 * | 1523 ± 289 * | 1907 ± 301 * | 2000 ± 269 * | |
dist. control | 271 ± 45 | 341 ± 193 | 603 ± 4 | 478 ± 203 | 640 ± 219 | |
PF | 70 ± 157 | 931 ± 344 | 1496 ± 342 | 1375 ± 399 | 1610 ± 469 | |
Capillaries | ||||||
prox. control | 1 ± 0 | 2 ± 0 | 3 ± 0 | 4 ± 1 | 6 ± 1 | |
PF | 2 ± 0 * | 4 ± 1 * | 6 ± 1 * | 9 ± 1 * | 13 ± 2 * | |
med. control | 1 ± 0 | 2 ± 0 | 3 ± 0 | 4 ± 1 | 5 ± 1 | |
PF | 2 ± 0 | 4 ± 0 * | 6 ± 1 * | 10 ± 1 * | 12 ± 2 | |
dist. control | 1 ± 0 | 2 ± 1 | 2 ± 0 | 4 ± 1 | 5 ± 0 | |
PF | 1 ± 0 | 3 ± 1 | 5 ± 1 * | 9 ± 3 | 11 ± 2 * | |
Venules | ||||||
prox. control | 441 ± 92 | 679 ± 161 | 1304 ± 307 | 1872 ± 452 | 2154 ± 491 | |
PF | 881 ± 287 | 1377 ± 261 * | 1500 ± 256 | 2568 ± 508 | 3060 ± 567 | |
med. control | 424 ± 116 | 537 ± 115 | 1020 ± 166 | 1675 ± 280 | 2713 ± 409 | |
PF | 539 ± 157 | 1443 ± 446 * | 1692 ± 262 | 3125 ± 533 * | 3767 ± 558 | |
dist. control | 197 ± 107 | 577 ± 131 | 637 ± 203 | 1184 ± 155 | 1565 ± 137 | |
PF | 514 ± 153 | 1088 ± 310 | 1768 ± 460 | 2650 ± 884 | 2484 ± 514 |
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Weinzierl, A.; Coerper, M.; Harder, Y.; Menger, M.D.; Laschke, M.W. Short-Term Periodic Fasting Reduces Ischemia-Induced Necrosis in Musculocutaneous Flap Tissue. Biomedicines 2024, 12, 690. https://doi.org/10.3390/biomedicines12030690
Weinzierl A, Coerper M, Harder Y, Menger MD, Laschke MW. Short-Term Periodic Fasting Reduces Ischemia-Induced Necrosis in Musculocutaneous Flap Tissue. Biomedicines. 2024; 12(3):690. https://doi.org/10.3390/biomedicines12030690
Chicago/Turabian StyleWeinzierl, Andrea, Maximilian Coerper, Yves Harder, Michael D. Menger, and Matthias W. Laschke. 2024. "Short-Term Periodic Fasting Reduces Ischemia-Induced Necrosis in Musculocutaneous Flap Tissue" Biomedicines 12, no. 3: 690. https://doi.org/10.3390/biomedicines12030690
APA StyleWeinzierl, A., Coerper, M., Harder, Y., Menger, M. D., & Laschke, M. W. (2024). Short-Term Periodic Fasting Reduces Ischemia-Induced Necrosis in Musculocutaneous Flap Tissue. Biomedicines, 12(3), 690. https://doi.org/10.3390/biomedicines12030690