Sulfated CXCR3 Peptide Trap Use as a Promising Therapeutic Approach for Age-Related Macular Degeneration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmid Construction
2.2. Protein Purification
2.3. Wound Healing Assay
2.4. Invasion Assay
2.5. RAW 264.7 Cell Culture
2.6. Experimental Animals
2.7. Laser-Induced CNV Mouse Models
2.8. Quantitative Reverse Transcriptase-Mediated Real-Time PCR (qPCR)
2.9. Quantitation of CNV in the Mouse Model of Laser-Induced CNV
2.10. Immunofluorescence Staining
2.11. Statistical Analysis
3. Results
3.1. Cloning and Purification of mCXCR3-S2 and hCXCR3-S2
3.2. hCXCR3 Sulfation Attenuated CXCL10-Induced Cell Migration and Invasion
3.3. mCXCR3-S2 Prevented CNV and Macrophage Infiltration in the Laser-Induced CNV Mouse Model
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jo, G.; Chae, J.-B.; Jung, S.-A.; Lyu, J.; Chung, H.; Lee, J.H. Sulfated CXCR3 Peptide Trap Use as a Promising Therapeutic Approach for Age-Related Macular Degeneration. Biomedicines 2024, 12, 241. https://doi.org/10.3390/biomedicines12010241
Jo G, Chae J-B, Jung S-A, Lyu J, Chung H, Lee JH. Sulfated CXCR3 Peptide Trap Use as a Promising Therapeutic Approach for Age-Related Macular Degeneration. Biomedicines. 2024; 12(1):241. https://doi.org/10.3390/biomedicines12010241
Chicago/Turabian StyleJo, Gukheui, Jae-Byoung Chae, Sun-Ah Jung, Jungmook Lyu, Hyewon Chung, and Joon H. Lee. 2024. "Sulfated CXCR3 Peptide Trap Use as a Promising Therapeutic Approach for Age-Related Macular Degeneration" Biomedicines 12, no. 1: 241. https://doi.org/10.3390/biomedicines12010241