Exploring Circulating Long Non-Coding RNAs in Mild Cognitive Impairment Patients’ Blood
Abstract
:1. Introduction
2. Materials and Methods
2.1. Recruitment of Patients
2.2. Blood Collection
2.3. RNA Extraction
2.4. lncRNA Microarray Profiling
2.5. Validation of lncRNA Gene Expression in Blood by Real-Time Quantitative PCR (RT-qPCR)
2.6. Statistical and Bioinformatics Analyses
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Clinical Characteristics | Value | MCI Patients | Healthy Controls |
---|---|---|---|
Age at our observation (years) | Mean ± STD | 59.07 ± 18.76 | 62.25 ± 11.26 |
Range | 49–65 | 52–66 | |
Age at symptom onset (years) | Mean ± STD | 55.17 ± 10.35 | - |
Range | 54–63 | - | |
Sex | Male | 4 | 5 |
Female | 6 | 5 | |
Mini-Mental State Examination (MMSE) score | - | 22.48 (±2.06) | 28.47 (±1.93) |
Other cerebrovascular pathology | - | None | None |
Metabolic/endocrine disease | - | None | None |
Rank | Name | Score |
---|---|---|
1 | SNHG16 | 281.0 |
2 | MALAT1 | 202.0 |
3 | H19 | 174.0 |
4 | HOTAIR | 153.0 |
5 | MEG3 | 135.0 |
6 | NEAT1 | 102.0 |
7 | TUG1 | 95.0 |
8 | XIST | 84.0 |
9 | GAS5 | 78.0 |
10 | UCA1 | 63.0 |
Description | Shared Name |
---|---|
post-transcriptional regulation of gene expression | GO:0010608 |
positive regulation of cell population proliferation | GO:0008284 |
regulation of epithelial cell proliferation | GO:0050678 |
epithelial cell proliferation | GO:0050673 |
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De Felice, B.; Coppola, C.; Bonavita, S.; Signoriello, E.; Montanino, C.; Farinella, F. Exploring Circulating Long Non-Coding RNAs in Mild Cognitive Impairment Patients’ Blood. Biomedicines 2023, 11, 2963. https://doi.org/10.3390/biomedicines11112963
De Felice B, Coppola C, Bonavita S, Signoriello E, Montanino C, Farinella F. Exploring Circulating Long Non-Coding RNAs in Mild Cognitive Impairment Patients’ Blood. Biomedicines. 2023; 11(11):2963. https://doi.org/10.3390/biomedicines11112963
Chicago/Turabian StyleDe Felice, Bruna, Cinzia Coppola, Simona Bonavita, Elisabetta Signoriello, Concetta Montanino, and Federica Farinella. 2023. "Exploring Circulating Long Non-Coding RNAs in Mild Cognitive Impairment Patients’ Blood" Biomedicines 11, no. 11: 2963. https://doi.org/10.3390/biomedicines11112963