Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination
Abstract
:1. Introduction
2. Materials and Methods
2.1. Blood Collection and RNA Extraction
2.2. Reverse Transcription and qPCR
2.3. Cell Culture
2.4. Transmission Electron Microscopy
2.5. Western Blot
3. Results
3.1. Study Participants
3.2. Vaccine mRNA Remains in Circulation for at Least 15 Days
3.3. WBCs Are Unlikely Candidates for S-Protein Expression In Vitro
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Participant ID | Age (Years) | Sex | Vaccine | Doses Received | Time (Days) | |||
---|---|---|---|---|---|---|---|---|
Between Doses | Blood Collection after 1st Dose | Blood Collection after 2nd Dose | Blood Collection after 3rd Dose | |||||
A1.1 | 37 | M | BNT162b2 | 1 | 1 | |||
B1.1 | 49 | F | BNT162b2 | 1 | 1 | |||
C1.2 | 37 | M | BNT162b2 | 1 | 2 | |||
D1.3 | 21 | F | BNT162b2 | 1 | 3 | |||
E1.3 | 21 | M | BNT162b2 | 1 | 3 | |||
F1.4 | 21 | F | BNT162b2 | 1 | 4 | |||
G1.4 | 21 | M | BNT162b2 | 1 | 4 | |||
H1.5 | 33 | F | BNT162b2 | 1 | 5 | |||
I1.6 | 21 | F | BNT162b2 | 1 | 6 | |||
G1.6 | 21 | M | BNT162b2 | 1 | 6 | |||
H2.0 | 33 | F | BNT162b2 | 2 | 21 | 0 | ||
J2.1 | 37 | F | BNT162b2 | 2 | 21 | 1 | ||
B2.2 | 49 | F | BNT162b2 | 2 | 21 | 2 | ||
K2.2 | 50 | F | BNT162b2 | 2 | 21 | 2 | ||
J2.2 | 37 | F | BNT162b2 | 2 | 21 | 2 | ||
C2.3 | 37 | M | BNT162b2 | 2 | 21 | 3 | ||
L2.3 | 37 | M | BNT162b2 | 2 | 21 | 3 | ||
M2.5 | 25 | F | BNT162b2 | 2 | 21 | 5 | ||
N2.5 | 34 | F | BNT162b2 | 2 | 21 | 5 | ||
O2.6 | 35 | F | BNT162b2 | 2 | 21 | 6 | ||
P2.7 | 21 | F | BNT162b2 | 2 | 21 | 7 | ||
B2.15 | 49 | F | BNT162b2 | 2 | 21 | 15 | ||
M2.27 | 25 | F | BNT162b2 | 2 | 21 | 27 | ||
CTRL1 | 31 | M | ||||||
CTRL2 | 68 | F | ||||||
CTRL3 | 39 | M | ||||||
B3 | 49 | F | BNT162b2 | 3 | 21/272 | 0, 1, 2, 4, 7, 9, 11, 14 |
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Fertig, T.E.; Chitoiu, L.; Marta, D.S.; Ionescu, V.-S.; Cismasiu, V.B.; Radu, E.; Angheluta, G.; Dobre, M.; Serbanescu, A.; Hinescu, M.E.; et al. Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines 2022, 10, 1538. https://doi.org/10.3390/biomedicines10071538
Fertig TE, Chitoiu L, Marta DS, Ionescu V-S, Cismasiu VB, Radu E, Angheluta G, Dobre M, Serbanescu A, Hinescu ME, et al. Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines. 2022; 10(7):1538. https://doi.org/10.3390/biomedicines10071538
Chicago/Turabian StyleFertig, Tudor Emanuel, Leona Chitoiu, Daciana Silvia Marta, Victor-Stefan Ionescu, Valeriu Bogdan Cismasiu, Eugen Radu, Giulia Angheluta, Maria Dobre, Ana Serbanescu, Mihail Eugen Hinescu, and et al. 2022. "Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination" Biomedicines 10, no. 7: 1538. https://doi.org/10.3390/biomedicines10071538
APA StyleFertig, T. E., Chitoiu, L., Marta, D. S., Ionescu, V. -S., Cismasiu, V. B., Radu, E., Angheluta, G., Dobre, M., Serbanescu, A., Hinescu, M. E., & Gherghiceanu, M. (2022). Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines, 10(7), 1538. https://doi.org/10.3390/biomedicines10071538