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Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination

Victor Babeș National Institute of Pathology, 050096 Bucharest, Romania
Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania
Faculty of Medicine, Titu Maiorescu University, 040441 Bucharest, Romania
Laboratory of Molecular Biology and Pathology Research, Emergency University Hospital Bucharest, 050098 Bucharest, Romania
Cantacuzino NIRDMM-Research and Development Center, 050096 Bucharest, Romania
Author to whom correspondence should be addressed.
Academic Editors: Isabella Quinti, Raffaele D’Amelio and Enrico Maggi
Biomedicines 2022, 10(7), 1538;
Received: 25 May 2022 / Revised: 15 June 2022 / Accepted: 24 June 2022 / Published: 28 June 2022
(This article belongs to the Special Issue Vaccines and Antibodies for Therapy and Prophylaxis)
COVID-19 mRNA vaccines effectively reduce incidence of severe disease, hospitalisation and death. The biodistribution and pharmacokinetics of the mRNA-containing lipid nanoparticles (LNPs) in these vaccines are unknown in humans. In this study, we used qPCR to track circulating mRNA in blood at different time-points after BNT162b2 vaccination in a small cohort of healthy individuals. We found that vaccine-associated synthetic mRNA persists in systemic circulation for at least 2 weeks. Furthermore, we used transmission electron microscopy (TEM) to investigate SARS-CoV-2 spike protein expression in human leukemic cells and in primary mononuclear blood cells treated in vitro with the BNT162b2 vaccine. TEM revealed morphological changes suggestive of LNP uptake, but only a small fraction of K562 leukemic cells presented spike-like structures at the cell surface, suggesting reduced levels of expression for these specific phenotypes. View Full-Text
Keywords: COVID-19; mRNA vaccine; lipid nanoparticles; biodistribution; spike protein COVID-19; mRNA vaccine; lipid nanoparticles; biodistribution; spike protein
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MDPI and ACS Style

Fertig, T.E.; Chitoiu, L.; Marta, D.S.; Ionescu, V.-S.; Cismasiu, V.B.; Radu, E.; Angheluta, G.; Dobre, M.; Serbanescu, A.; Hinescu, M.E.; Gherghiceanu, M. Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines 2022, 10, 1538.

AMA Style

Fertig TE, Chitoiu L, Marta DS, Ionescu V-S, Cismasiu VB, Radu E, Angheluta G, Dobre M, Serbanescu A, Hinescu ME, Gherghiceanu M. Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines. 2022; 10(7):1538.

Chicago/Turabian Style

Fertig, Tudor Emanuel, Leona Chitoiu, Daciana Silvia Marta, Victor-Stefan Ionescu, Valeriu Bogdan Cismasiu, Eugen Radu, Giulia Angheluta, Maria Dobre, Ana Serbanescu, Mihail Eugen Hinescu, and Mihaela Gherghiceanu. 2022. "Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination" Biomedicines 10, no. 7: 1538.

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