Next Article in Journal
Novel Inflammasome-Based Risk Score for Predicting Survival and Efficacy to Immunotherapy in Early-Stage Non-Small Cell Lung Cancer
Next Article in Special Issue
Recent Progress in the Discovery and Development of Monoclonal Antibodies against Viral Infections
Previous Article in Journal
Role of Wnt Signaling in Mouse Fetal Skin Wound Healing
 
 
Article

Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination

1
Victor Babeș National Institute of Pathology, 050096 Bucharest, Romania
2
Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania
3
Faculty of Medicine, Titu Maiorescu University, 040441 Bucharest, Romania
4
Laboratory of Molecular Biology and Pathology Research, Emergency University Hospital Bucharest, 050098 Bucharest, Romania
5
Cantacuzino NIRDMM-Research and Development Center, 050096 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Academic Editors: Isabella Quinti, Raffaele D’Amelio and Enrico Maggi
Biomedicines 2022, 10(7), 1538; https://doi.org/10.3390/biomedicines10071538
Received: 25 May 2022 / Revised: 15 June 2022 / Accepted: 24 June 2022 / Published: 28 June 2022
(This article belongs to the Special Issue Vaccines and Antibodies for Therapy and Prophylaxis)
COVID-19 mRNA vaccines effectively reduce incidence of severe disease, hospitalisation and death. The biodistribution and pharmacokinetics of the mRNA-containing lipid nanoparticles (LNPs) in these vaccines are unknown in humans. In this study, we used qPCR to track circulating mRNA in blood at different time-points after BNT162b2 vaccination in a small cohort of healthy individuals. We found that vaccine-associated synthetic mRNA persists in systemic circulation for at least 2 weeks. Furthermore, we used transmission electron microscopy (TEM) to investigate SARS-CoV-2 spike protein expression in human leukemic cells and in primary mononuclear blood cells treated in vitro with the BNT162b2 vaccine. TEM revealed morphological changes suggestive of LNP uptake, but only a small fraction of K562 leukemic cells presented spike-like structures at the cell surface, suggesting reduced levels of expression for these specific phenotypes. View Full-Text
Keywords: COVID-19; mRNA vaccine; lipid nanoparticles; biodistribution; spike protein COVID-19; mRNA vaccine; lipid nanoparticles; biodistribution; spike protein
Show Figures

Figure 1

MDPI and ACS Style

Fertig, T.E.; Chitoiu, L.; Marta, D.S.; Ionescu, V.-S.; Cismasiu, V.B.; Radu, E.; Angheluta, G.; Dobre, M.; Serbanescu, A.; Hinescu, M.E.; Gherghiceanu, M. Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines 2022, 10, 1538. https://doi.org/10.3390/biomedicines10071538

AMA Style

Fertig TE, Chitoiu L, Marta DS, Ionescu V-S, Cismasiu VB, Radu E, Angheluta G, Dobre M, Serbanescu A, Hinescu ME, Gherghiceanu M. Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines. 2022; 10(7):1538. https://doi.org/10.3390/biomedicines10071538

Chicago/Turabian Style

Fertig, Tudor Emanuel, Leona Chitoiu, Daciana Silvia Marta, Victor-Stefan Ionescu, Valeriu Bogdan Cismasiu, Eugen Radu, Giulia Angheluta, Maria Dobre, Ana Serbanescu, Mihail Eugen Hinescu, and Mihaela Gherghiceanu. 2022. "Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination" Biomedicines 10, no. 7: 1538. https://doi.org/10.3390/biomedicines10071538

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop